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Western Blot (WB) (Western BlotPositive WB detected in: K562 whole cell lysate, Hela whole cell lysate, SH-SY5Y whole cell lysate, PC3 whole cell lysateAll lanes: LYAR antibody at 2.5ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 44 KDaObserved band size: 44 KDa)

Rabbit anti-Human LYAR Polyclonal Antibody | anti-LYAR antibody

LYAR Antibody

Gene Names
LYAR; ZLYAR; ZC2HC2
Reactivity
Human
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
>95%,Protein G purified
Synonyms
LYAR; Polyclonal Antibody; LYAR Antibody; Cell growth-regulating nucleolar protein; PNAS-5; anti-LYAR antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
>95%,Protein G purified
Form/Format
Liquid
Sequence Length
379
Applicable Applications for anti-LYAR antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Immunogen
Recombinant human Cell growth-regulating nucleolar protein
Conjugation
Non-conjugated
Storage Buffer
Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-160503
Preparation and Storage
Shipped at 4 degree C. Upon delivery, aliquot and store at -20 degree C or -80 degree C.

Western Blot (WB)

(Western BlotPositive WB detected in: K562 whole cell lysate, Hela whole cell lysate, SH-SY5Y whole cell lysate, PC3 whole cell lysateAll lanes: LYAR antibody at 2.5ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 44 KDaObserved band size: 44 KDa)

Western Blot (WB) (Western BlotPositive WB detected in: K562 whole cell lysate, Hela whole cell lysate, SH-SY5Y whole cell lysate, PC3 whole cell lysateAll lanes: LYAR antibody at 2.5ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 44 KDaObserved band size: 44 KDa)

Immunohistochemistry (IHC)

(IHC image of MBS7002835 diluted at 1:500 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)

Immunohistochemistry (IHC) (IHC image of MBS7002835 diluted at 1:500 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)

Immunohistochemistry (IHC)

(Immunofluorescence staining of Hela cells with MBS7002835 at 1:166,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).)

Immunohistochemistry (IHC) (Immunofluorescence staining of Hela cells with MBS7002835 at 1:166,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).)
Related Product Information for anti-LYAR antibody
nucleolus, nucleus, poly(A) RNA binding

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
43,615 Da
NCBI Official Full Name
cell growth-regulating nucleolar protein
NCBI Official Synonym Full Names
Ly1 antibody reactive
NCBI Official Symbol
LYAR
NCBI Official Synonym Symbols
ZLYAR; ZC2HC2
NCBI Protein Information
cell growth-regulating nucleolar protein
UniProt Protein Name
Cell growth-regulating nucleolar protein
UniProt Gene Name
LYAR
UniProt Entry Name
LYAR_HUMAN

Uniprot Description

LYAR:

Protein type: Unknown function; Nucleolus

Chromosomal Location of Human Ortholog: 4p16.3

Cellular Component: nucleolus; nucleus

Molecular Function: metal ion binding; protein binding

Research Articles on LYAR

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Product Notes

The LYAR lyar (Catalog #AAA7002835) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The LYAR Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's LYAR can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). Researchers should empirically determine the suitability of the LYAR lyar for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LYAR, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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