Rat E3 SUMO-protein ligase PIAS3 ELISA Kit | Pias3 elisa kit

Rat E3 SUMO-protein ligase PIAS3 ELISA Kit

Cat. Num. AAA2884565

• Reactivity: Rat
• Synonyms: E3 SUMO-protein ligase PIAS3; Rat E3 SUMO-protein ligase PIAS3 ELISA Kit; KChAP; Potassium channel-associated protein; Protein inhibitor of activated STAT protein 3; Pias3; Pias3 elisa kit

• Reactivity: Rat
• Sequence Length: 628

• Assay Type: Sandwich
• Detection Range: 78-5000 pg/mL
• Sensitivity: 41.3 pg/mL
• Intra-Assay CV: <=5.3%
• Inter-Assay CV: <=8.6%
• Recovery: 97%
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

NCBI and Uniprot Product Information

• NCBI GI #: 17298668
• NCBI GeneID: 83614
• NCBI Accession #: NP_113972.2
• NCBI GenBank Nucleotide #: NM_031784.2
• UniProt Accession #: O70260
• Molecular Weight: 68,364 Da
• NCBI Official Full Name: E3 SUMO-protein ligase PIAS3
• NCBI Official Synonym Full Names: protein inhibitor of activated STAT, 3
• NCBI Official Symbol: Pias3
• NCBI Protein Information: E3 SUMO-protein ligase PIAS3
• UniProt Protein Name: E3 SUMO-protein ligase PIAS3
• Protein Family: E3 SUMO-protein ligase
• UniProt Gene Name: Pias3
• UniProt Entry Name: PIAS3_RAT

NCBI Description

voltage-gated K+ channel binding protein; involved in modulating the expression of Kv2 channels [RGD, Feb 2006]

Uniprot Description

PIAS3: Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway and the steroid hormone signaling pathway. Involved in regulating STAT3 signaling via inhibiting STAT3 DNA-binding and suppressing cell growth. Monomer. Binds SUMO1 and UBE2I. Interacts with BCL11A, HMGA2, IRF1, MITF and NCOA2. Interacts with STAT5; the interaction occurs on stimulation by PRL. Interacts with GFI1; the interaction relieves the inhibitory effect of PIAS3 on STAT3- mediated transcriptional activity. Interacts with AR, PLAG1 and ZFHX3. Interacts with STAT3; the interaction occurs on stimulation by IL6, CNTF or OSM and inhibits the DNA binding activity of STAT3. By dihydrotestosterone (DHT) in prostate cancer cells. Isoform 1 is expressed in most tissues except thymus and small intestine. Isoform 3 is expressed only in brain, heart, thymus, muscle, lung, testis, lactating breast and embryonic stem cells. Belongs to the PIAS family.

Protein type: EC 6.3.2.-; SUMO conjugating system; Nuclear receptor co-regulator

Cellular Component: cytoplasm; dendrite; nuclear speck; nucleus; synapse

Molecular Function: enzyme binding; ligase activity; potassium channel regulator activity; protein binding; protein C-terminus binding; protein N-terminus binding; SUMO ligase activity; zinc ion binding

Biological Process: negative regulation of protein sumoylation; positive regulation of membrane potential; positive regulation of protein sumoylation; protein sumoylation; regulation of transcription, DNA-dependent; response to hormone stimulus; transcription, DNA-dependent