BLMH / BLM hydrolase Recombinant Protein | BLMH recombinant protein

Recombinant Human BLMH / BLM hydrolase Protein (His tag)

Cat. Num. AAA2545805

• Gene Names: BLMH; BH; BMH
• Purity: > 88 % as determined by SDS-PAGE
• Synonyms: BLMH / BLM hydrolase; Recombinant Human BLMH / BLM hydrolase Protein (His tag); NME1; AWD; GAAD; NB; NBS; NDPK-A; NDPKA; NM23; NM23-H1; BLMH recombinant protein

• Host: E Coli
• Purity/Purification: > 88 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 455

• Application Notes: The recombinant human BLMH consisting of 461 amino acids and has a calculated molecular mass of 53.4 kDa as estimated in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Met
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for BLMH recombinant protein

Background: The papain superfamily member bleomycin hydrolase (BLMH) is a cytoplasmic cysteine peptidase that is highly conserved through evolution. The only known activity of the enzyme is metabolic inactivation of the glycopeptide bleomycin (BLM), an essential component of combination chemotherapy regimens for cancer. The papain superfamily member bleomycin hydrolase (BLMH) is a neutral cysteine protease with structural similarity to a 20S proteasome. Bleomycin (BLM), a clinically used glycopeptide anticancer agent. BLMH is an essential protectant against BLM-induced death and has an important role in neonatal survival and in maintaining epidermal integrity. Sequencing revealed several putative sites phosphorylated by different type s of protein kinases, but no signal sequence, transmembrane domain, N-linked glycosylation site or DNA-binding motif.

Description: A DNA sequence encoding the human BLMH (NP_000377.1) (Ser 2-Glu 455) was expressed, with a polyhistide tag at the N-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 4557367
• NCBI GeneID: 642
• NCBI Accession #: NP_000377.1
• NCBI GenBank Nucleotide #: NM_000386.3
• UniProt Accession #: Q13867; Q53F86; Q9UER9; B2R796
• Molecular Weight: 52,562 Da
• NCBI Official Full Name: bleomycin hydrolase
• NCBI Official Synonym Full Names: bleomycin hydrolase
• NCBI Official Symbol: BLMH
• NCBI Official Synonym Symbols: BH; BMH
• NCBI Protein Information: bleomycin hydrolase
• UniProt Protein Name: Bleomycin hydrolase
• Protein Family: Bleomycin hydrolase
• UniProt Gene Name: BLMH
• UniProt Synonym Gene Names: BH; BLM hydrolase; BMH

NCBI Description

Bleomycin hydrolase (BMH) is a cytoplasmic cysteine peptidase that is highly conserved through evolution; however, the only known activity of the enzyme is metabolic inactivation of the glycopeptide bleomycin (BLM), an essential component of combination chemotherapy regimens for cancer. The protein contains the signature active site residues of the cysteine protease papain superfamily. [provided by RefSeq, Jul 2008]

Uniprot Description

BLMH: The normal physiological role of BLM hydrolase is unknown, but it catalyzes the inactivation of the antitumor drug BLM (a glycopeptide) by hydrolyzing the carboxamide bond of its B- aminoalaninamide moiety thus protecting normal and malignant cells from BLM toxicity. Belongs to the peptidase C1 family.

Protein type: EC 3.4.22.40; Protease

Chromosomal Location of Human Ortholog: 17q11.2

Cellular Component: cytoplasm; cytosol; nucleus

Molecular Function: aminopeptidase activity; carboxypeptidase activity; cysteine-type peptidase activity; identical protein binding; protein binding

Biological Process: homocysteine catabolic process; protein polyubiquitination; response to toxin

Disease: Alzheimer Disease