Rabbit anti-Human B3GAT1 Polyclonal Antibody | anti-B3GAT1 antibody

B3GAT1, NT (B3GAT1, GLCATP, Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1, Beta-1,3-glucuronyltransferase 1, Glucuronosyltransferase P, UDP-GlcUA:glycoprotein beta-1,3-glucuronyltransferase) (PE)

Cat. Num. AAA6277106

• Gene Names: B3GAT1; NK1; CD57; HNK1; LEU7; NK-1; GLCATP; GLCUATP
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: B3GAT1; Polyclonal Antibody; NT (B3GAT1; GLCATP; Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1; Beta-1; 3-glucuronyltransferase 1; Glucuronosyltransferase P; UDP-GlcUA:glycoprotein beta-1; 3-glucuronyltransferase) (PE); 3-glucuronyltransferase; anti-B3GAT1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-B3GAT1 antibody: Flow Cytometry (FC/FACS), FLISA, Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: B3GAT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 27~57 amino acids from the N-terminal region of human B3GAT1.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of B3GAT1 Antibody (N-term) in K562 cell line lysates (35ug/lane). B3GAT1 (arrow) was detected using the purified Pab.)

Flow Cytometry (FC/FACS)

(B3GAT1 Antibody (N-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-B3GAT1 antibody

The protein encoded by this gene is a member of the glucuronyltransferase gene family. These enzymes exhibit strict acceptor specificity, recognizing nonreducing terminal sugars and their anomeric linkages. This gene product functions as the key enzyme in a glucuronyl transfer reaction during the biosynthesis of the carbohydrate epitope HNK-1 (human natural killer-1, also known as CD57 and LEU7).

Product Categories/Family for anti-B3GAT1 antibody

Antibodies; Enzymes; Glucuronosyltransferase

References

Petrovas, C., et al. J. Immunol. 183(2):1120-1132(2009). Saito, A., et al. J. Hum. Genet. 54(6):317-323(2009). Chong, L.K., et al. Eur. J. Immunol. 38(4):995-1000(2008). Casado, J.G., et al. Tumour Biol. 29(5):304-310(2008).

NCBI and Uniprot Product Information

• NCBI GI #: 77695914
• NCBI GeneID: 27087
• NCBI Accession #: NP_061114.2
• NCBI GenBank Nucleotide #: NP_061114.2
• UniProt Accession #: Q9P2W7
• NCBI Official Full Name: galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1 isoform 1
• NCBI Official Synonym Full Names: beta-1,3-glucuronyltransferase 1
• NCBI Official Symbol: B3GAT1
• NCBI Official Synonym Symbols: NK1; CD57; HNK1; LEU7; NK-1; GLCATP; GLCUATP
• NCBI Protein Information: galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1
• UniProt Protein Name: Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1
• Protein Family: Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase
• UniProt Gene Name: B3GAT1
• UniProt Synonym Gene Names: GlcUAT-P

NCBI Description

The protein encoded by this gene is a member of the glucuronyltransferase gene family. These enzymes exhibit strict acceptor specificity, recognizing nonreducing terminal sugars and their anomeric linkages. This gene product functions as the key enzyme in a glucuronyl transfer reaction during the biosynthesis of the carbohydrate epitope HNK-1 (human natural killer-1, also known as CD57 and LEU7). Alternate transcriptional splice variants have been characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

Involved in the biosynthesis of L2/HNK-1 carbohydrate epitope on glycoproteins. Can also play a role in glycosaminoglycan biosynthesis. Substrates include asialo-orosomucoid (ASOR), asialo-fetuin, and asialo-neural cell adhesion molecule. Requires sphingomyelin for activity: stearoyl-sphingomyelin was the most effective, followed by palmitoyl-sphingomyelin and lignoceroyl-sphingomyelin. Activity was demonstrated only for sphingomyelin with a saturated fatty acid and not for that with an unsaturated fatty acid, regardless of the length of the acyl group.