Rabbit Bid Polyclonal Antibody | anti-BID antibody

Anti-Bid Antibody

Cat. Num. AAA176831

• Gene Names: BID; FP497
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Immunogen affinity purified.
• Synonyms: Bid; Polyclonal Antibody; Anti-Bid Antibody; BH3-interacting domain death agonist; BH3 interacting domain death agonist; Apoptic death agonist antibody; Apoptotic death agonist antibody; Apoptotic death agonist BID antibody; BH3 interacting domain death agonist antibody; BH3 interacting domain death agonist p11 antibody; BH3 interacting domain death agonist p13 antibody; BH3 interacting domain death agonist p15 antibody; BH3-interacting domain death agonist p11 antibody; BID antibody; BID isoform ES(1b) antibody; BID isoform L(2) antibody; BID isoform Si6 antibody; BID_HUMAN antibody; Desmocollin type 4 antibody; FP497 antibody; Human BID coding sequence antibody; MGC15319 antibody; MGC42355 antibody; p11 BID antibody; p13 BID antibody; p15 BID antibody; p22 BID antibody; anti-BID antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Lyophilized
• Sequence Length: 195

• Applicable Applications for anti-BID antibody: Western Blot (WB), Immunohistochemistry (IHC) Paraffin
• Immunogen: E Coli-derived human Bid recombinant protein (Position: M1-D195). Human Bid shares 64% and 61% amino acid (aa) sequences identity with mouse and rat Bid, respectively.
• Contents: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Immunohistochemistry (IHC)

(Anti-Bid Picoband antibody, MBS176831-1.JPGIHC(P): Human Mammary Cancer Tissue )

Immunohistochemistry (IHC)

(Anti-Bid Picoband antibody, MBS176831-2.JPGIHC(P): Mouse Spleen Tissue )

Immunohistochemistry (IHC)

(Anti-Bid Picoband antibody, MBS176831-3.JPGIHC(P): Rat Intestine Tissue )

Immunocytochemistry (ICC)

(Anti-Bid Picoband antibody, MBS176831-4.JPGICC: A549 Cell )

Testing Data

(Anti-Bid Picoband antibody, MBS176831-5.jpgAll lanes: Anti-BID(MBS176831) at 0.5ug/mlLane 1: HELA Whole Cell Lysate at 40ugLane 2: COLO320 Whole Cell Lysate at 40ugLane 3: JURKAT Whole Cell Lysate at 40ugLane 4: SKOV Whole Cell Lysate at 40ugPredicted bind size: 22KDObserved bind size: 22KD)

Related Product Information for anti-BID antibody

Description: Rabbit IgG polyclonal antibody for BH3-interacting domain death agonist(BID) detection. Tested with WB, IHC-P in Human, Mouse, Rat.
Background: BID (BH3-Interacting Domain Death Agonist), is a pro-apoptotic member of the Bcl-2 protein family. The BCL2 family of proteins consists of both antagonists and agonists that regulate apoptosis and compete through dimerization. By fluorescence in situ hybridization, Wang et al. (1998) mapped the human BID gene to 22q11. Luo et al. (1998) reported the purification of a cytosolic protein that induces cytochrome c release from mitochondria in response to caspase-8, the apical caspase activated by cell surface death receptors such as FAS and TNF.

NCBI and Uniprot Product Information

• NCBI GI #: 2493285
• NCBI GeneID: 637
• UniProt Accession #: P55957; Q549M7; Q71T04; Q7Z4M9; Q8IY86
• Molecular Weight: 11,263 Da
• NCBI Official Full Name: BH3-interacting domain death agonist
• NCBI Official Synonym Full Names: BH3 interacting domain death agonist
• NCBI Official Symbol: BID
• NCBI Official Synonym Symbols: FP497
• NCBI Protein Information: BH3-interacting domain death agonist; p22 BID; BID isoform Si6; BID isoform L(2); BID isoform ES(1b); desmocollin type 4; apoptic death agonist; Human BID coding sequence
• UniProt Protein Name: BH3-interacting domain death agonist
• Protein Family: Bidirectional sugar transporter
• UniProt Gene Name: BID
• UniProt Synonym Gene Names: BID
• UniProt Entry Name: BID_HUMAN

NCBI Description

This gene encodes a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2. The encoded protein is a member of the BCL-2 family of cell death regulators. It is a mediator of mitochondrial damage induced by caspase-8 (CASP8); CASP8 cleaves this encoded protein, and the COOH-terminal part translocates to mitochondria where it triggers cytochrome c release. Multiple alternatively spliced transcript variants have been found, but the full-length nature of some variants has not been defined. [provided by RefSeq, Jul 2008]

Uniprot Description

BID: a pro-apoptotic member of the Bcl-2 superfamily. Targets intracellular membranes and contains a BH3 death domain. Heterodimerizes with either the pro-apoptotic protein BAX or the anti-apoptotic protein BCL2, antagonizing its protective effect. The activity of BID is regulated by Caspase 8-mediated cleavage, exposing the BH3 domain and significantly changing the surface charge and hydrophobicity, causing translocation to mitochondria where it triggers cytochrome c release. Multiple alternatively spliced variants have been found.

Protein type: Mitochondrial; Apoptosis

Chromosomal Location of Human Ortholog: 22q11.1

Cellular Component: mitochondrial outer membrane; membrane; mitochondrion; cytosol

Molecular Function: protein binding; ubiquitin protein ligase binding; death receptor binding

Biological Process: caspase activation; release of cytochrome c from mitochondria; positive regulation of apoptosis; positive regulation of protein homooligomerization; apoptosis; DNA damage response, signal transduction; response to estradiol stimulus; regulation of cell proliferation; positive regulation of protein oligomerization; neuron apoptosis; apoptotic mitochondrial changes; induction of apoptosis via death domain receptors; brain development; protein targeting to mitochondrion; protein homooligomerization