Goat beta2-Glycoprotein-1 (APO-H) Polyclonal Antibody | anti-APOH antibody

Goat anti-human Apolipoprotein-H (beta2GPI), Whole IgG from antiserum

Cat. Num. AAA512023

• Gene Names: APOH; BG; B2G1; B2GP1
• Purity: Purified IgG.; Vial containing ml of whole IgG representing approximately 1 ml of antiserum. Total protein is 5 mg.
• Synonyms: beta2-Glycoprotein-1 (APO-H); Polyclonal Antibody; Goat anti-human Apolipoprotein-H (beta2GPI); Whole IgG from antiserum; human; anti-APOH antibody

• Host: Goat
• Clonality: Polyclonal
• Specificity: This antibody is specific for beta2GPI as demonstrated by immunoelectrophoresis and ELISA.
• Purity/Purification: Purified IgG.; Vial containing ml of whole IgG representing approximately 1 ml of antiserum. Total protein is 5 mg.
• Form/Format: Whole IgG, clear liquid.
• Concentration: IgG concentration is mg/ml, determined by absorbance using an extinction coefficient (E1%280) of 13.4. (varies by lot)

• Application Notes: Suitable as a source of antibodies to human beta2GPI.
• Immunogen: Human beta2GPI purified from plasma.
• Buffer: 10 mM HEPES, pH 7.4, 150 mM NaCl, 50% (v/v) glycerol.
• Neutralizing Activity: Not determined
• Preparation and Storage: Store between -10 and -20 degree C. Product will become viscous but will not freeze. Avoid storage in frost-free freezers. Keep vial tightly capped. Allow product to warm to room temperature and gently mix before use.

Related Product Information for anti-APOH antibody

Apolipoprotein-H, also known as beta2-Glycoprotein I (beta2GPI), is a plasma glycoprotein that circulates at a concentration of 200 ug/ml (4 uM). Synthesized in the liver, beta2GPI is a single chain molecule of 48 kDa, consisting of five repeating internally disulphide-bonded structures referred to as sushi domains. Relative to other glycoproteins, beta2GPI has an unusually high content of cysteine (6.2%), proline (8.3%) and carbohydrate (19%). Almost half the circulating beta2GPI in plasma is associated with lipoproteins of all major fractions. beta2GPI has been demonstrated to bind negatively charged phospholipids, heparin and platelets. Although the precise function(s) are as yet unknown, beta2GPI has been demonstrated to interfere with blood coagulation by competitively binding to negatively charged phospholipid surfaces exposed during cell activation or damage. Recent evidence also implicates beta2GPI as a cofactor recognized by anti-phospholipid antibodies present in some autoimmune disorders such as systemic lupus erythematosus (SLE)1-3.

References

1. Lee, NS, Brewer HB Jr., Osborne JC Jr.; beta2Glycoprotein I: Molecular Properties of an Unusual Apolipoprotein, Apolipoprotein H; JBC 258, pp 4765-4770, 1983.
2. Schousboe I; beta2Glycoprotein I: A Plasma Inhibitor of the Contact Activation of the Intrinsic Blood Coagulation Pathway. Blood 66, pp 1 086-1091, 1985.
3. Nimpf J, Bevers EM, Bomans PHH, Till U, Wurm H, Kostner GM, Zwaal RFA; Prothrombinase activity of human platelets is inhibited by beta2Glycoprotein I; Biochimica et Biophysica Acta 884, pp 142-149, 1986.

NCBI and Uniprot Product Information

• NCBI GI #: 153266841
• NCBI GeneID: 350
• NCBI Accession #: NP_000033.2
• NCBI GenBank Nucleotide #: NM_000042.2
• UniProt Accession #: P02749; Q9UCN7; B2R9M3
• Molecular Weight: 38,298 Da
• NCBI Official Full Name: beta-2-glycoprotein 1
• NCBI Official Synonym Full Names: apolipoprotein H (beta-2-glycoprotein I)
• NCBI Official Symbol: APOH
• NCBI Official Synonym Symbols: BG; B2G1; B2GP1
• NCBI Protein Information: beta-2-glycoprotein 1; B2GPI; apo-H; beta(2)GPI; APC inhibitor; anticardiolipin cofactor; activated protein C-binding protein
• UniProt Protein Name: Beta-2-glycoprotein 1
• Protein Family: Beta-2-glycoprotein
• UniProt Gene Name: APOH
• UniProt Synonym Gene Names: B2G1
• UniProt Entry Name: APOH_HUMAN

NCBI Description

Apolipoprotein H has been implicated in a variety of physiologic pathways including lipoprotein metabolism, coagulation, and the production of antiphospholipid autoantibodies. APOH may be a required cofactor for anionic phospholipid binding by the antiphospholipid autoantibodies found in sera of many patients with lupus and primary antiphospholipid syndrome, but it does not seem to be required for the reactivity of antiphospholipid autoantibodies associated with infections. [provided by RefSeq]

Uniprot Description

Function: Binds to various kinds of negatively charged substances such as heparin, phospholipids, and dextran sulfate. May prevent activation of the intrinsic blood coagulation cascade by binding to phospholipids on the surface of damaged cells.

Subcellular location: Secreted.

Tissue specificity: Expressed by the liver and secreted in plasma.

Post-translational modification: N- and O-glycosylated. Ref.10 also reports glycosylation on 'Asn-188' for their allele. Ref.10 Ref.15 Ref.16 Ref.17 Ref.18 Ref.19 Ref.20 Ref.21

Sequence similarities: Contains 4 Sushi (CCP/SCR) domains.