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Western Blot (WB) (Western blotAll lanes: AP1M2 antibody at 2ug/mlLane 1:mouse small intestine tissueLane 2:mouse kidney tisueSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 48kDaObserved band size: 48kDa)

Rabbit anti-Human, Mouse AP1M2 Polyclonal Antibody | anti-AP1M2 antibody

AP1M2 Antibody

Gene Names
AP1M2; mu2; MU1B; MU-1B; HSMU1B; AP1-mu2
Reactivity
Human, Mouse
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Antigen Affinity Purified
Synonyms
AP1M2; Polyclonal Antibody; AP1M2 Antibody; AP-mu chain family member mu1B; Adaptor protein complex AP-1 subunit mu-2; Adaptor-related protein complex 1 subunit mu-2; Clathrin assembly protein complex 1 mu-2 medium chain 2; Golgi adaptor HA1/AP1 adaptin mu-2 subunit; Mu-adaptin 2; Mu1B-adaptin; anti-AP1M2 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Antigen Affinity Purified
Sequence Length
425
Applicable Applications for anti-AP1M2 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Recommended dilution: WB:1:500-2000
IHC:1:20-1:200
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Conjugate
Non-conjugated
Immunogen
Recombinant human AP-1 complex subunit mu-2 protein (164-423aa)
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-69446
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(Western blotAll lanes: AP1M2 antibody at 2ug/mlLane 1:mouse small intestine tissueLane 2:mouse kidney tisueSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 48kDaObserved band size: 48kDa)

Western Blot (WB) (Western blotAll lanes: AP1M2 antibody at 2ug/mlLane 1:mouse small intestine tissueLane 2:mouse kidney tisueSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 48kDaObserved band size: 48kDa)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human breast cancer using MBS1494956 at dilution 1:100)

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human breast cancer using MBS1494956 at dilution 1:100)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human kidney using MBS1494956 at dilution 1:100)

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human kidney using MBS1494956 at dilution 1:100)
Related Product Information for anti-AP1M2 antibody
Subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the trans-Golgi network (TGN) and endosomes. The AP complexes mediate the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
48,210 Da
NCBI Official Full Name
AP-1 complex subunit mu-2 isoform 1
NCBI Official Synonym Full Names
adaptor related protein complex 1 mu 2 subunit
NCBI Official Symbol
AP1M2
NCBI Official Synonym Symbols
mu2; MU1B; MU-1B; HSMU1B; AP1-mu2
NCBI Protein Information
AP-1 complex subunit mu-2
UniProt Protein Name
AP-1 complex subunit mu-2
Protein Family
UniProt Gene Name
AP1M2
UniProt Entry Name
AP1M2_HUMAN

NCBI Description

This gene encodes a subunit of the heterotetrameric adaptor-related protein comlex 1 (AP-1), which belongs to the adaptor complexes medium subunits family. This protein is capable of interacting with tyrosine-based sorting signals. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]

Uniprot Description

AP1M2: Subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the trans-Golgi network (TGN) and endosomes. The AP complexes mediate the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules. Belongs to the adaptor complexes medium subunit family. 2 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: clathrin adaptor complex; cytoplasmic vesicle membrane; cytosol; Golgi membrane; intracellular membrane-bound organelle; lysosomal membrane; trans-Golgi network membrane

Molecular Function: protein binding

Biological Process: antigen processing and presentation of exogenous peptide antigen via MHC class II; post-Golgi vesicle-mediated transport; protein targeting; regulation of defense response to virus by virus; vesicle targeting; viral reproduction

Research Articles on AP1M2

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Product Notes

The AP1M2 ap1m2 (Catalog #AAA1494956) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The AP1M2 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's AP1M2 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). Recommended dilution: WB:1:500-2000 IHC:1:20-1:200. Researchers should empirically determine the suitability of the AP1M2 ap1m2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "AP1M2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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