Rabbit Bcl-XL, XS Polyclonal Antibody | anti-BCL2L1 antibody

Bcl-XL, XS

Cat. Num. AAA649331

• Gene Names: BCL2L1; BCLX; BCL2L; BCLXL; BCLXS; Bcl-X; bcl-xL; bcl-xS; PPP1R52; BCL-XL/S
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunoprecipitation, Immunohistochemistry
• Purity: Serum; Serum
• Synonyms: Bcl-XL; XS; Polyclonal Antibody; Anti -Bcl-XL; anti-BCL2L1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human Bcl-XL and Bcl-XS. Species Crossreactivity: gerbil, mouse, rat.
• Purity/Purification: Serum; Serum
• Form/Format: Supplied as a liquid, 0.05% sodium azide.

• Applicable Applications for anti-BCL2L1 antibody: Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Western Blot, Immunohistochemistry and Immunoprecipitation.; Dilution: Western Blot: 1:1000-1:2000; Immunohistochemistry (formalin fixed paraffin embedded): 1:1000-1:5000; Immunoprecipitation: 1:50-1:200; Immunohistochemistry: Frozen
• Immunogen: Synthetic peptide corresponding to aa61-79 (DSPAVNGATAHSSSLDARE) of human Bcl-XL; GenBank no. CAA80661.1.
• Positive Control: CNS lymphatic tissues, many cancer cell lines
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(Formalin-fixed paraffin-embedded section of dog ischemic brain cortex stained for Bcl-X expression at 1:2000. At 2hr post ischemia, Bcl-X staining was seen in the dying neurons that had morphological features of apoptosis (arrows). In contrast, mophologically normal appearing neurons lacked Bcl-X staining (arrowheads). Hematoxylin-eosin counterstain.)

Immunohistochemistry (IHC)

(Formalin-fixed paraffin- embedded human tissue sections stained for Bcl-X expression using MBS649331 at 1:2000. A. Cortex from the brain of an Alzheimer's disease patient. The degenerating neurons are positive for BclX expression, the intact neurons are not. B. Normal bone marrow. The cells positive for Bcl-X expression appear to be of erythropoetic lineage. Hematoxylin-eosin counterstain.)

Related Product Information for anti-BCL2L1 antibody

The Bcl-2 family of apoptosis-related genes plays central roles in regulating apoptotic pathways (reviewed in Thomadaki and Scorilas, 2006). Regulation of cell death through apoptosis is critical for the maintenance of homeostasis, defense against infectious agents, and normal development. Bcl-2 family proteins regulate apoptosis primarily through the regulation of mitochondrial outer membrane permeability. In mammals, the family consists of both prosurvival (antiapoptotic) and proapoptotic (prodeath) members. Cellular homeostasis is thought to be dependent on a balance between the actions of prosurvival and proapoptotic proteins. Bcl-2 family proteins can be divided into 3 main subfamilies on the basis of their function and the content of their Bcl-2 homology (BH) domains, for example: 1) Prosurvival: Bcl-2, Bcl-XL, Bcl-W, A1, and Mcl-1 2) Proapoptotic (multidomain): Bax, Bak, and Bok. 3) BH3-only (proapoptotic): Bad, Bcl-XS, Bid, Bik, Bim, Blk, Bmf, Bnip, Noxa, and Puma. Prosurvival members inhibit cells from undergoing apoptosis, whereas proapoptotic and BH3-only subfamily members promote apoptosis. There are 4 BH domains (1-4) conserved among Bcl-2 family proteins. The BH domains are important for function as well as for heterodimerization between family members. Typical prosurvival family members have all four BH domains (1-4), whereas proapoptotic (multidomain) members have BH1, 2 and 3 domains and BH3-only members have only the BH3 domain. Overall, the relative ratio of prosurvival and proapoptotic proteins determines the suseptibility of a cell to various apoptotic stimuli. Many Bcl-2 family proteins are differentially expressed in various malignancies and some are useful prognostic biomarkers. Prosurvival proteins are often elevated in diverse cancers and have the potential to confer resistance to both endogenous cell death stimuli and cancer treatments. Alterations in the ratio or levels of Bcl-2 family proteins have been also associated with nonmalignant diseases including neurodegenerative diseases, autoimmune diseases, AIDs, Down's syndrome, cardiovascular diseases, diabetes, glomerulonephritis, and muscular dystrophy. Recognizes Bcl-XL and Bcl-XS. Human Bcl-XL is a 233aa protein; GenBank no. CAA80661.1. Human Bcl-XS is a 170aa protein; GenBank no. CAA80662.1.

Product Categories/Family for anti-BCL2L1 antibody

Antibodies; Abs to Apoptosis

NCBI and Uniprot Product Information

• NCBI GI #: 4502381
• NCBI GeneID: 598
• NCBI Accession #: NP_001182.1
• NCBI GenBank Nucleotide #: NM_001191.2
• UniProt Accession #: Q07817; Q5CZ89; Q5TE65; Q92976; E1P5L6
• Molecular Weight: 26,049 Da
• NCBI Official Full Name: bcl-2-like protein 1 isoform 2
• NCBI Official Synonym Full Names: BCL2-like 1
• NCBI Official Symbol: BCL2L1
• NCBI Official Synonym Symbols: BCLX; BCL2L; BCLXL; BCLXS; Bcl-X; bcl-xL; bcl-xS; PPP1R52; BCL-XL/S
• NCBI Protein Information: bcl-2-like protein 1; apoptosis regulator Bcl-X; protein phosphatase 1, regulatory subunit 52
• UniProt Protein Name: Bcl-2-like protein 1
• Protein Family: Bcl-2-like protein
• UniProt Gene Name: BCL2L1
• UniProt Synonym Gene Names: BCL2L; BCLX; Bcl2-L-1
• UniProt Entry Name: B2CL1_HUMAN

NCBI Description

The protein encoded by this gene belongs to the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The proteins encoded by this gene are located at the outer mitochondrial membrane, and have been shown to regulate outer mitochondrial membrane channel (VDAC) opening. VDAC regulates mitochondrial membrane potential, and thus controls the production of reactive oxygen species and release of cytochrome C by mitochondria, both of which are the potent inducers of cell apoptosis. Two alternatively spliced transcript variants, which encode distinct isoforms, have been reported. The longer isoform acts as an apoptotic inhibitor and the shorter form acts as an apoptotic activator. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Potent inhibitor of cell death. Inhibits activation of caspases

By similarity. Appears to regulate cell death by blocking the voltage-dependent anion channel (VDAC) by binding to it and preventing the release of the caspase activator, CYC1, from the mitochondrial membrane. Also acts as a regulator of G2 checkpoint and progression to cytokinesis during mitosis. Ref.17 Ref.19Isoform Bcl-X(S) promotes apoptosis. Ref.17 Ref.19

Subunit structure: Homodimer. Isoform Bcl-X(L) forms heterodimers with BAX, BAK or BCL2. Heterodimerization with BAX does not seem to be required for anti-apoptotic activity. Interacts with BCL2L11. Interacts with DMN1L; the interaction stimulates the GTPase activity of DMN1L in synapses and increases the number of axonal mitochondria and the size and number of synaptic vesicle clusters

By similarity. Interacts with BAD. Interacts (isoform Bcl-X(L)) with SIVA1 (isoform 1); the interaction inhibits the anti-apoptotic activity. Interacts with BECN1 and PGAM5. Interacts (isoform Bcl-X(L)) with BAX (isoform Sigma). Isoform Bcl-X(L) interacts with IKZF3. Interacts with HEBP2. Isoform Bcl-X(L) interacts with BOP/C22orf29. Interacts with P53/TP53 and BBC3; interaction with BBC3 disrupts the interaction with P53/TP53. Ref.2 Ref.9 Ref.12 Ref.13 Ref.14 Ref.15 Ref.16 Ref.25 Ref.28 Ref.29

Subcellular location: Mitochondrion membrane; Single-pass membrane protein. Nucleus membrane; Single-pass membrane protein; Cytoplasmic side

By similarity. Cytoplasm › cytoskeleton › microtubule organizing center › centrosome. Note: Mitochondrial membranes and perinuclear envelope

By similarity. Localizes to the centrosome when phosphorylated at Ser-49. Ref.17 Ref.19

Tissue specificity: Bcl-X(S) is expressed at high levels in cells that undergo a high rate of turnover, such as developing lymphocytes. In contrast, Bcl-X(L) is found in tissues containing long-lived postmitotic cells, such as adult brain.

Domain: The BH4 motif is required for anti-apoptotic activity. The BH1 and BH2 motifs are required for both heterodimerization with other Bcl-2 family members and for repression of cell death.

Post-translational modification: Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity. Ref.11Phosphorylated on Ser-62 by CDK1. This phosphorylation is partial in normal mitotic cells, but complete in G2-arrested cells upon DNA-damage, thus promoting subsequent apoptosis probably by triggering caspases-mediated proteolysis. Phosphorylated by PLK3, leading to regulate the G2 checkpoint and progression to cytokinesis during mitosis. Phosphorylation at Ser-49 appears during the S phase and G2, disappears rapidly in early mitosis during prometaphase, metaphase and early anaphase, and re-appears during telophase and cytokinesis. Ref.17 Ref.19

Sequence similarities: Belongs to the Bcl-2 family.