CREB Peptide | CREB1 peptide

CREB, phosphorylated (Ser133) (cAMP Response Element Binding Protein) Blocking Peptide

Cat. Num. AAA659747

• Gene Names: CREB1; CREB
• Applications: ELISA, Western Blot
• Purity: Purified; Purified
• Synonyms: CREB; phosphorylated (Ser133) (cAMP Response Element Binding Protein) Blocking Peptide; CREB1 peptide

• Purity/Purification: Purified; Purified
• Form/Format: Supplied as a lyophilized powder.

• Applicable Applications for CREB1 peptide: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA, Western Blot or for antigen applications in immunological protocols.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degree C or colder. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis of extracts from forskolin and IBMX treated SK-N-MC cells, using C7915-10Awith and without preincubation with phospho-peptide.)

Immunohistochemistry (IHC)

(Immunohistochemical staining of paraffin-embedded human breast carcinoma using Phospho-CREB (Ser133) Antibody preincubated with irrelevant control peptide (left) and C7915-10A(right).)

Related Product Information for CREB1 peptide

Transcription factor CREB binds the cyclic AMP response element (CRE) and activates transcription in response to a variety of extracellular signals including neurotransmitters, hormones, membrane depolarization, and growth or neurotrophic factors. Protein kinase A and the calmodulin-dependent protein kinase CaMKII stimulate CREB phosphorylation at Ser133, a key regulatory site controlling transcriptional activity (1,2). Phosphorylation at Ser133 is also mediated by p44/42 MAP kinase, p90RSK, p38 MAP kinase and MSK1 (3-5). CREB appears to play an important role in learning and memory in both flies and mice.

Produced by immunizing rabbits with a synthetic phosphopeptide (KLH coupled) corresponding to residues around Ser133 of human CREB.

Product Categories/Family for CREB1 peptide

Molecular Biology; MB-Transcription Factors

NCBI and Uniprot Product Information

• NCBI GI #: 33694240
• NCBI GeneID: 1385
• UniProt Accession #: P16220; P21934; Q6V963; Q9UMA7
• Molecular Weight: 36,688 Da
• NCBI Official Full Name: CREB
• NCBI Official Synonym Full Names: cAMP responsive element binding protein 1
• NCBI Official Symbol: CREB1
• NCBI Official Synonym Symbols: CREB
• NCBI Protein Information: cyclic AMP-responsive element-binding protein 1; CREB-1; transactivator protein; active transcription factor CREB; cAMP-response element-binding protein-1; cAMP-responsive element-binding protein 1
• UniProt Protein Name: Cyclic AMP-responsive element-binding protein 1
• Protein Family: CREB-binding protein
• UniProt Gene Name: CREB1
• UniProt Synonym Gene Names: CREB-1
• UniProt Entry Name: CREB1_HUMAN

NCBI Description

This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Phosphorylation-dependent transcription factor that stimulates transcription upon binding to the DNA cAMP response element (CRE), a sequence present in many viral and cellular promoters. Transcription activation is enhanced by the TORC coactivators which act independently of Ser-133 phosphorylation. Involved in different cellular processes including the synchronization of circadian rhythmicity and the differentiation of adipose cells.

Subunit structure: Interacts with PPRC1. Binds DNA as a dimer. This dimer is stabilized by magnesium ions. Interacts, through the bZIP domain, with the coactivators TORC1/CRTC1, TORC2/CRTC2 and TORC3/CRTC3. When phosphorylated on Ser-133, binds CREBBP

By similarity. Interacts with CREBL2; regulates CREB1 phosphorylation, stability and transcriptional activity

By similarity. Interacts (phosphorylated form) with TOX3. Interacts with ARRB1. Binds to HIPK2. Interacts with SGK1. Ref.9 Ref.10 Ref.15 Ref.17 Ref.18 Ref.19 Ref.20 Ref.21 Ref.26 Ref.27

Subcellular location: Nucleus Ref.16.

Post-translational modification: Stimulated by phosphorylation. Phosphorylation of both Ser-133 and Ser-142 in the SCN regulates the activity of CREB and participates in circadian rhythm generation. Phosphorylation of Ser-133 allows CREBBP binding

By similarity. CREBL2 positively regulates phosphorylation at Ser-133 thereby stimulating CREB1 transcriptional activity

By similarity. Phosphorylated upon calcium influx by CaMK4 and CaMK2 on Ser-133. CaMK4 is much more potent than CaMK2 in activating CREB. Phosphorylated by CaMK2 on Ser-142. Phosphorylation of Ser-142 blocks CREB-mediated transcription even when Ser-133 is phosphorylated. Phosphorylated by CaMK1

By similarity. Phosphorylation of Ser-271 by HIPK2 in response to genotoxic stress promotes CREB1 activity, facilitating the recruitment of the coactivator CBP. Phosphorylated at Ser-133 by RPS6KA3, RPS6KA4 and RPS6KA5 in response to mitogenic or stress stimuli. Ref.11 Ref.12 Ref.13 Ref.14 Ref.20 Ref.26Sumoylated with SUMO1. Sumoylation on Lys-304, but not on Lys-285, is required for nuclear localization of this protein. Sumoylation is enhanced under hypoxia, promoting nuclear localization and stabilization. Ref.16

Involvement in disease: Angiomatoid fibrous histiocytoma (AFH) [MIM:612160]: A distinct variant of malignant fibrous histiocytoma that typically occurs in children and adolescents and is manifest by nodular subcutaneous growth. Characteristic microscopic features include lobulated sheets of histiocyte-like cells intimately associated with areas of hemorrhage and cystic pseudovascular spaces, as well as a striking cuffing of inflammatory cells, mimicking a lymph node metastasis.Note: The gene represented in this entry may be involved in disease pathogenesis. A chromosomal aberration involving CREB1 is found in a patient with angiomatoid fibrous histiocytoma. Translocation t(2;22)(q33;q12) with CREB1 generates a EWSR1/CREB1 fusion gene that is most common genetic abnormality in this tumor type.A CREB1 mutation has been found in a patient with multiple congenital anomalies consisting of agenesis of the corpus callosum, cerebellar hypoplasia, severe neonatal respiratory distress refractory to surfactant, thymus hypoplasia, and thyroid follicular hypoplasia (Ref.29).

Sequence similarities: Belongs to the bZIP family.Contains 1 bZIP (basic-leucine zipper) domain.Contains 1 KID (kinase-inducible) domain.