Rabbit Bcl-XL, XS Polyclonal Antibody | anti-BCL2L1 antibody

Bcl-XL, XS

Cat. Num. AAA6010506

• Gene Names: BCL2L1; BCLX; BCL2L; BCLXL; BCLXS; Bcl-X; bcl-xL; bcl-xS; PPP1R52; BCL-XL/S
• Reactivity: Canine, Human, Mouse, Rat
• Applications: Western Blot, Immunoprecipitation, Immunohistochemistry
• Purity: Serum; Serum
• Synonyms: Bcl-XL; XS; Polyclonal Antibody; Anti -Bcl-XL; anti-BCL2L1 antibody

• Host: Rabbit
• Reactivity: Canine, Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human Bcl-XL and Bcl-XS. Species Crossreactivity: canine, gerbil, mouse, rat.
• Purity/Purification: Serum; Serum
• Form/Format: Supplied as a liquid, 0.05% sodium azide.

• Applicable Applications for anti-BCL2L1 antibody: Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Western Blot, Immunohistochemistry and Immunoprecipitation.; Dilution: Western Blot: 1:1000-1:2000; Immunohistochemistry (formalin fixed paraffin embedded): 1:1000-1:5000; Immunoprecipitation: 1:50-1:200; Immunohistochemistry: Frozen
• Immunogen: Recombinant corresponding to human Bcl-XL protein without the N-terminal transmembrane portion.
• Positive Control: CNS lymphatic tissues, many cancer cell lines
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of Bcl-X. Lane 1. lysates from isolated rat liver mitochondria. Lanes 2-4. rat brain tissue lysates: cortex (lane 2), basal ganglia (lane 3), and cerebellum (lane 4). The antibody detected Bcl-XL at ~30kD.)

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded human colon carcinoma tissue section stained for Bcl-X expression using MBS6010506 at 1:2000. Hematoxylin-eosin counterstain.)

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded normal human colon epithelium tissue section stained for Bcl-X expression using MBS6010506 at 1:2000. Hematoxylin-eosin counterstain. A course granular Bcl-X staining pattern associated with the mitochondria is observed.)

Related Product Information for anti-BCL2L1 antibody

The Bcl-2 family of apoptosis-related genes plays central roles in regulating apoptotic pathways (reviewed in Thomadaki and Scorilas, 2006). Regulation of cell death through apoptosis is critical for the maintenance of homeostasis, defense against infectious agents, and normal development. Bcl-2 family proteins regulate apoptosis primarily through the regulation of mitochondrial outer membrane permeability. In mammals, the family consists of both prosurvival (antiapoptotic) and proapoptotic (prodeath) members. Cellular homeostasis is thought to be dependent on a balance between the actions of prosurvival and proapoptotic proteins. Bcl-2 family proteins can be divided into 3 main subfamilies on the basis of their function and the content of their Bcl-2 homology (BH) domains, for example: 1) Prosurvival: Bcl-2, Bcl-XL, Bcl-W, A1, and Mcl-1 2) Proapoptotic (multidomain): Bax, Bak, and Bok. 3) BH3-only (proapoptotic): Bad, Bcl-XS, Bid, Bik, Bim, Blk, Bmf, Bnip, Noxa, and Puma. Prosurvival members inhibit cells from undergoing apoptosis, whereas proapoptotic and BH3-only subfamily members promote apoptosis. There are 4 BH domains (1-4) conserved among Bcl-2 family proteins. The BH domains are important for function as well as for heterodimerization between family members. Typical prosurvival family members have all four BH domains (1-4), whereas proapoptotic (multidomain) members have BH1, 2 and 3 domains and BH3-only members have only the BH3 domain. Overall, the relative ratio of prosurvival and proapoptotic proteins determines the suseptibility of a cell to various apoptotic stimuli. Many Bcl-2 family proteins are differentially expressed in various malignancies and some are useful prognostic biomarkers. Prosurvival proteins are often elevated in diverse cancers and have the potential to confer resistance to both endogenous cell death stimuli and cancer treatments. Alterations in the ratio or levels of Bcl-2 family proteins have been also associated with nonmalignant diseases including neurodegenerative diseases, autoimmune diseases, AIDs, Down's syndrome, cardiovascular diseases, diabetes, glomerulonephritis, and muscular dystrophy. Human Bcl-XL is a 233aa protein; GenBank no. CAA80661.1. Human Bcl-XS is a 170aa protein; GenBank no. CAA80662.1.

Product Categories/Family for anti-BCL2L1 antibody

Antibodies; Abs to Apoptosis

NCBI and Uniprot Product Information

• NCBI GI #: 4502381
• NCBI GeneID: 598
• NCBI Accession #: NP_001182.1
• NCBI GenBank Nucleotide #: NM_001191.2
• UniProt Accession #: Q07817; Q5CZ89; Q5TE65; Q92976; E1P5L6
• Molecular Weight: 26,049 Da
• NCBI Official Full Name: bcl-2-like protein 1 isoform 2
• NCBI Official Synonym Full Names: BCL2-like 1
• NCBI Official Symbol: BCL2L1
• NCBI Official Synonym Symbols: BCLX; BCL2L; BCLXL; BCLXS; Bcl-X; bcl-xL; bcl-xS; PPP1R52; BCL-XL/S
• NCBI Protein Information: bcl-2-like protein 1; apoptosis regulator Bcl-X; protein phosphatase 1, regulatory subunit 52
• UniProt Protein Name: Bcl-2-like protein 1
• Protein Family: Bcl-2-like protein
• UniProt Gene Name: BCL2L1
• UniProt Synonym Gene Names: BCL2L; BCLX; Bcl2-L-1
• UniProt Entry Name: B2CL1_HUMAN

NCBI Description

The protein encoded by this gene belongs to the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The proteins encoded by this gene are located at the outer mitochondrial membrane, and have been shown to regulate outer mitochondrial membrane channel (VDAC) opening. VDAC regulates mitochondrial membrane potential, and thus controls the production of reactive oxygen species and release of cytochrome C by mitochondria, both of which are the potent inducers of cell apoptosis. Two alternatively spliced transcript variants, which encode distinct isoforms, have been reported. The longer isoform acts as an apoptotic inhibitor and the shorter form acts as an apoptotic activator. [provided by RefSeq, Jul 2008]

Uniprot Description

Bcl-xL: an antiapoptotic member of the Bcl-2 family. Located at the outer mitochondrial membrane and regulates outer mitochondrial membrane channel (VDAC) opening. VDAC regulates mitochondrial membrane potential, and thus controls the production of reactive oxygen species and release of cytochrome C by mitochondria, both of which are potent inducers of cell apoptosis. Two alternatively spliced isoforms have been reported.

Protein type: Membrane protein, integral; Mitochondrial; Apoptosis; Autophagy

Chromosomal Location of Human Ortholog: 20q11.21

Cellular Component: centrosome; nuclear membrane; synaptic vesicle membrane; mitochondrion; integral to membrane; cytosol; mitochondrial outer membrane; mitochondrial matrix; cytoplasm; mitochondrial inner membrane; nucleolus; cell junction; nucleus

Molecular Function: identical protein binding; protein binding; protein homodimerization activity; protein heterodimerization activity; BH3 domain binding; protein kinase binding

Biological Process: negative regulation of autophagy; release of cytochrome c from mitochondria; apoptosis; in utero embryonic development; mitotic cell cycle checkpoint; male gonad development; suppression by virus of host apoptosis; cytokinesis; endocytosis; cellular process regulating host cell cycle in response to virus; germ cell development; cell proliferation; neuron apoptosis; apoptotic mitochondrial changes; fertilization; regulation of mitochondrial membrane potential; DNA damage response, signal transduction resulting in induction of apoptosis; ovarian follicle development; response to cytokine stimulus; positive regulation of cell proliferation; innate immune response; response to cycloheximide; spermatogenesis; negative regulation of neuron apoptosis; regulation of mitochondrial membrane permeability; growth; negative regulation of apoptosis