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Typical Testing Data/Standard Curve (for reference only)

Human serpin peptidase inhibitor, clade B (ovalbumin) , member 5 ELISA Kit | SERPINB5 elisa kit

Human Serpin B5, SERPINB5 ELISA Kit

Gene Names
SERPINB5; PI5; maspin
Reactivity
Human
Synonyms
serpin peptidase inhibitor; clade B (ovalbumin); member 5; Human Serpin B5; SERPINB5 ELISA Kit; Human Serpin B5 (SERPINB5) ELISA kit; PI5; maspin; protease inhibitor 5 (maspin) ; serine (or cysteine) proteinase inhibitor; SERPINB5 elisa kit
Ordering
For Research Use Only!
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of human SERPINB5. No significant cross-reactivity or interference between human SERPINB5 and analogues was observed.
Sequence Length
375
Samples
Serum, plasma, tissue homogenates, cell lysates
Assay Type
Quantitative Sandwich
Detection Range
0.156 ng/ml-10 ng/ml
Sensitivity
< 0.039 ng/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)
Related Product Information for SERPINB5 elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SERPINB5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SERPINB5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SERPINB5 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SERPINB5 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
42,100 Da
NCBI Official Full Name
serpin B5
NCBI Official Synonym Full Names
serpin peptidase inhibitor, clade B (ovalbumin), member 5
NCBI Official Symbol
SERPINB5
NCBI Official Synonym Symbols
PI5; maspin
NCBI Protein Information
serpin B5; PI-5; peptidase inhibitor 5; protease inhibitor 5 (maspin); serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 5
UniProt Protein Name
Serpin B5
Protein Family
UniProt Gene Name
SERPINB5
UniProt Synonym Gene Names
PI5; PI-5
UniProt Entry Name
SPB5_HUMAN

Uniprot Description

maspin: Tumor suppressor. It blocks the growth, invasion, and metastatic properties of mammary tumors. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity. Interacts with IRF6. Normal mammary epithelial cells. Belongs to the serpin family. Ov-serpin subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 18q21.33

Cellular Component: extracellular space; cytoplasm

Molecular Function: serine-type endopeptidase inhibitor activity; protein binding

Biological Process: extracellular matrix organization and biogenesis; morphogenesis of an epithelium; cell motility; regulation of epithelial cell proliferation

Research Articles on SERPINB5

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Product Notes

The Human SERPINB5 serpinb5 (Catalog #AAA911955) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA911955 ELISA Kit recognizes Human SERPINB5. It is sometimes possible for the material contained within the vial of "serpin peptidase inhibitor, clade B (ovalbumin) , member 5, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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