Western Blot (WB)
(Western Blot: Sample: Lane1: Human Lung lysate; Lane2: Hela cell lysate; Lane3: A549 cell lysatePrimary Ab: 2ug/ml Rabbit Anti-Human TITF1 AntibodySecond Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody(#MBS2086047))
Rabbit anti-Human Thyroid Transcription Factor 1 (TITF1) Polyclonal Antibody | anti-TITF1 antibody
Thyroid Transcription Factor 1 (TITF1) Polyclonal Antibody
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
ELISA: 0.05-2ug/ml
Western Blot (WB)
(Western Blot: Sample: Lane1: Human Lung lysate; Lane2: Hela cell lysate; Lane3: A549 cell lysatePrimary Ab: 2ug/ml Rabbit Anti-Human TITF1 AntibodySecond Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody(#MBS2086047))
Western Blot (WB)
(Western Blot: Sample: Lane1: Human Lung lysate; Lane2: Hela cell lysate; Lane3: A549 cell lysatePrimary Ab: 2ug/ml Rabbit Anti-Human TITF1 AntibodySecond Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody(#MBS2086047))
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a protein initially identified as a thyroid-specific transcription factor. The encoded protein binds to the thyroglobulin promoter and regulates the expression of thyroid-specific genes but has also been shown to regulate the expression of genes involved in morphogenesis. Mutations and deletions in this gene are associated with benign hereditary chorea, choreoathetosis, congenital hypothyroidism, and neonatal respiratory distress, and may be associated with thyroid cancer. Multiple transcript variants encoding different isoforms have been found for this gene. This gene shares the symbol/alias 'TTF1' with another gene, transcription termination factor 1, which plays a role in ribosomal gene transcription. [provided by RefSeq, Feb 2014]
Uniprot Description
NKX2-1: a homeobox transcription factor expressed in thyroid, lung, and parts of the brain. Essential for the branching morphogenesis of the lung. Binds and activates the promoters of CEACAM6, thyroid-specific genes, ROR1, LAMP3, VEGF, etc. Its transcription is responsive to SMAD2 signaling after endodermal differentiation is induced in embryonic stem cell. Defects in NKX2-1 are associated with neurological, thyroid, and respiratory problems. Frequent amplified in human lung cancer cell lines and tumors. Amplified in 15% of non-small cell lung cancers (NSCLC), and appears to drive cancer cell proliferation and survival. EGFR may be a downstream effector of NKX2-1 when amplified in NSCLC. May operate as a lineage-specific oncogene in lung adenocarcinoma. Belongs to the NK-2 homeobox family. Forms a regulatory loop with GRHL2 that coordinates lung epithelial cell morphogenesis and differentiation. Activates the transcription of GNRHR and plays a role in enhancing the circadian oscillation of its gene expression. Represses the transcription of the circadian transcriptional repressor NR1D1. Belongs to the NK-2 homeobox family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: DNA-binding; Transcription factor
Chromosomal Location of Human Ortholog: 14q13.3
Cellular Component: nucleoplasm; nucleus; transcription factor complex
Molecular Function: DNA binding; DNA binding transcription factor activity; enzyme binding; intronic transcription regulatory region DNA binding; protein binding; transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding; transcription regulatory region DNA binding
Biological Process: anatomical structure formation involved in morphogenesis; axon guidance; brain development; cerebral cortex cell migration; cerebral cortex GABAergic interneuron differentiation; developmental induction; endoderm development; forebrain development; forebrain dorsal/ventral pattern formation; forebrain neuron fate commitment; globus pallidus development; hippocampus development; Leydig cell differentiation; locomotory behavior; lung development; negative regulation of cell migration; negative regulation of epithelial to mesenchymal transition; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; negative regulation of transforming growth factor beta receptor signaling pathway; neuron migration; oligodendrocyte differentiation; phospholipid metabolic process; pituitary gland development; positive regulation of circadian rhythm; positive regulation of gene expression; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-templated; response to hormone; rhythmic process; thyroid gland development; transcription, DNA-dependent
Disease: Chorea, Benign Hereditary; Choreoathetosis And Congenital Hypothyroidism With Or Without Pulmonary Dysfunction; Thyroid Carcinoma, Papillary
Research Articles on TITF1
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Product Notes
The TITF1 nkx2-1 (Catalog #AAA2090432) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Thyroid Transcription Factor 1 (TITF1) Polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Thyroid Transcription Factor 1 (TITF1) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), IHC, Immunocytochemistry (ICC), Immunoprecipitation (IP). Immunocytochemistry in formalin fixed cells: 5-20ug/ml Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml Immunohistochemistry in paraffin section: 5-20ug/ml ELISA: 0.05-2ug/ml. Researchers should empirically determine the suitability of the TITF1 nkx2-1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Thyroid Transcription Factor 1 (TITF1), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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