Bcl-xL Polyclonal Antibody | anti-Bcl-xL antibody

Bcl-xL (Phospho-Ser62)

Cat. Num. AAA191077

• Gene Names: BCL2L1; BCLX; BCL2L; Bcl-X; PPP1R52; BCL-XL/S
• Applications: Immunoblot, Western Blot, Immunohistochemistry, Immunofluorescence
• Synonyms: Bcl-xL; Polyclonal Antibody; Bcl-xL (Phospho-Ser62); anti-Bcl-xL antibody

• Clonality: Polyclonal
• Specificity: This antibody detects endogenous human, mouse, and rat Bcl-xL only when phosphorylated at serine 62.
• Form/Format: 100ul at 1.0mg/ml in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Affinity-purified on phosphopeptide; non-phosphopeptide- reactive antibodies were removed by chromatography on non-phosphorylated peptide.
• Sequence Length: 233

• Applicable Applications for anti-Bcl-xL antibody: Immunoblotting (IB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: Immunoblotting: use at dilution of 1:500-1:1,000. A band of ~30kDa is detected.; Immunohistochemistry: use at dilution of 1:50-1:100.; Immunofluorescence: use at dilution of 1:100-1:200.; Dilute in PBS or medium that is identical to that used in the assay system.
• Antigen: Peptide sequence that includes phosphorylation site of serine 62 derived from human Bcl-xL and conjugated to KLH.
• Preparation and Storage: This antibody is stable for at least one (1) year at -20 degree C. Can be stored at 4 degree C for short-term.

Immunofluorescence (IF)

Immunohistochemistry (IHC)

Testing Data

Related Product Information for anti-Bcl-xL antibody

B-cell lymphoma-extra large (Bcl-xl, or BCL2-like 1 isoform 1) is a transmembrane protein in the mitochondria. It is a member of the Bcl-2 family of proteins and acts as a pro-survival protein by preventing the release of mitochondrial cytochrome c, which would lead to caspase activation. Relative amounts of pro- and anti-survival Bcl-2 family proteins define whether a cell will undergo cell death: if more Bcl-xL is present, then pores are non-permeable to pro-apoptotic molecules and the cell survives.

NCBI and Uniprot Product Information

• NCBI GI #: 20336335
• NCBI GeneID: 598
• NCBI Accession #: NP_612815.1
• NCBI GenBank Nucleotide #: NM_138578.2
• UniProt Accession #: Q07817; Q5CZ89; Q5TE65; Q92976; E1P5L6
• Molecular Weight: 25,290 Da
• NCBI Official Full Name: bcl-2-like protein 1 isoform Bcl-X(L)
• NCBI Official Synonym Full Names: BCL2 like 1
• NCBI Official Symbol: BCL2L1
• NCBI Official Synonym Symbols: BCLX; BCL2L; Bcl-X; PPP1R52; BCL-XL/S
• NCBI Protein Information: bcl-2-like protein 1
• UniProt Protein Name: Bcl-2-like protein 1
• UniProt Gene Name: BCL2L1
• UniProt Synonym Gene Names: BCL2L; BCLX; Bcl2-L-1

NCBI Description

The protein encoded by this gene belongs to the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The proteins encoded by this gene are located at the outer mitochondrial membrane, and have been shown to regulate outer mitochondrial membrane channel (VDAC) opening. VDAC regulates mitochondrial membrane potential, and thus controls the production of reactive oxygen species and release of cytochrome C by mitochondria, both of which are the potent inducers of cell apoptosis. Alternative splicing results in multiple transcript variants encoding two different isoforms. The longer isoform acts as an apoptotic inhibitor and the shorter isoform acts as an apoptotic activator. [provided by RefSeq, Dec 2015]

Uniprot Description

Bcl-xL: an antiapoptotic member of the Bcl-2 family. Located at the outer mitochondrial membrane and regulates outer mitochondrial membrane channel (VDAC) opening. VDAC regulates mitochondrial membrane potential, and thus controls the production of reactive oxygen species and release of cytochrome C by mitochondria, both of which are potent inducers of cell apoptosis. Two alternatively spliced isoforms have been reported.

Protein type: Apoptosis; Autophagy; Membrane protein, integral; Mitochondrial

Chromosomal Location of Human Ortholog: 20q11.21

Cellular Component: centrosome; cytoplasm; mitochondrial outer membrane; mitochondrion

Molecular Function: BH3 domain binding; identical protein binding; protein binding; protein heterodimerization activity; protein homodimerization activity; protein kinase binding

Biological Process: apoptotic mitochondrial changes; cytokinesis; mitotic cell cycle checkpoint; negative regulation of apoptosis; regulation of mitochondrial membrane permeability; regulation of mitochondrial membrane potential; release of cytochrome c from mitochondria; response to cytokine stimulus; suppression by virus of host apoptosis