Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Heterogeneous nuclear ribonucleoprotein I (hnRNP I/PTB) Standard Curve.)
Human Heterogeneous Nuclear Ribonucleoprotein I (hnRNP I/PTB) ELISA Kit | hnRNP I/PTB elisa kit
Human Heterogeneous Nuclear Ribonucleoprotein I (hnRNP I/PTB) ELISA Kit
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Heterogeneous nuclear ribonucleoprotein I (hnRNP I/PTB) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Heterogeneous nuclear ribonucleoprotein I (hnRNP I/PTB) Standard Curve.)
Background: hnRNP I/PTB belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA-binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. Heterogeneous nuclear ribonucleoprotein I encoded by hnRNP I/PTB has four repeats of quasi-RNA recognition motif (RRM) domains that bind RNAs. This protein binds to the intronic polypyrimidine tracts that requires pre-mRNA splicing and acts via the protein degradation ubiquitin-proteasome pathway. It may also promote the binding of U2 snRNP to pre-mRNAs. Heterogeneous nuclear ribonucleoprotein I is localized in the nucleoplasm and it is also detected in the perinucleolar structure. Alternatively spliced transcript variants encoding different isoforms have been described.
NCBI and Uniprot Product Information
NCBI Description
This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA-binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has four repeats of quasi-RNA recognition motif (RRM) domains that bind RNAs. This protein binds to the intronic polypyrimidine tracts that requires pre-mRNA splicing and acts via the protein degradation ubiquitin-proteasome pathway. It may also promote the binding of U2 snRNP to pre-mRNAs. This protein is localized in the nucleoplasm and it is also detected in the perinucleolar structure. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]
Uniprot Description
hnRNP I: a ubiquitously expressed heterogeneous nuclear ribonucleoprotein (hnRNP). hnRNPs are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. Binds to the polypyrimidine tract of introns. May promote the binding of U2 snRNP to pre-mRNA. Two alternatively spliced isoforms have been described.
Protein type: RNA-binding; RNA splicing
Chromosomal Location of Human Ortholog: 19p13.3
Cellular Component: nucleoplasm; membrane; nucleolus
Molecular Function: protein binding; poly-pyrimidine tract binding; RNA binding; nucleotide binding
Biological Process: nuclear mRNA splicing, via spliceosome; negative regulation of RNA splicing; negative regulation of nuclear mRNA splicing, via spliceosome; RNA splicing; negative regulation of muscle cell differentiation; gene expression; mRNA processing; regulation of alternative nuclear mRNA splicing, via spliceosome
Research Articles on hnRNP I/PTB
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Product Notes
The Human hnRNP I/PTB ptbp1 (Catalog #AAA9714741) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9714741 ELISA Kit recognizes Human hnRNP I/PTB. It is sometimes possible for the material contained within the vial of "Heterogeneous Nuclear Ribonucleoprotein I (hnRNP I/PTB), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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