Western Blot (WB)
(Western blot analysis of extracts from HeLa cells untreated or treated with EGF using Rabbit anti Bcl-xL (pSer62) antibody. Cells were cultured in serum free media for 16-20 hours before treatment with EGF (100ng/ml) for 30 minutes)
Rabbit Bcl-xL Polyclonal Antibody | anti-BCL2L1 antibody
Rabbit anti Bcl-xL (pSer62)
Storage in frost-free freezers is not recommended. This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 12 months from date of despatch
Western Blot (WB)
(Western blot analysis of extracts from HeLa cells untreated or treated with EGF using Rabbit anti Bcl-xL (pSer62) antibody. Cells were cultured in serum free media for 16-20 hours before treatment with EGF (100ng/ml) for 30 minutes)
Western Blot (WB)
(Western blot analysis of extracts from HeLa cells untreated or treated with EGF using Rabbit anti Bcl-xL (pSer62) antibody. Cells were cultured in serum free media for 16-20 hours before treatment with EGF (100ng/ml) for 30 minutes)
Bcl-xL inhibits apoptosis by preventing the release of mitochondrial cytochrome c and the subsequent activation of caspases 3 and 9.
Mitotic arrest induces complete phosphorylation of Bcl-xL at serine 62 by cyclin dependent kinase 1 (CDK1), inactivating the anti-apoptotic function of Bcl-xL (Terrano et al. 2010).
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene belongs to the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The proteins encoded by this gene are located at the outer mitochondrial membrane, and have been shown to regulate outer mitochondrial membrane channel (VDAC) opening. VDAC regulates mitochondrial membrane potential, and thus controls the production of reactive oxygen species and release of cytochrome C by mitochondria, both of which are the potent inducers of cell apoptosis. Alternative splicing results in multiple transcript variants encoding two different isoforms. The longer isoform acts as an apoptotic inhibitor and the shorter isoform acts as an apoptotic activator. [provided by RefSeq, Dec 2015]
Uniprot Description
Potent inhibitor of cell death. Inhibits activation of caspases. Appears to regulate cell death by blocking the voltage-dependent anion channel (VDAC) by binding to it and preventing the release of the caspase activator, CYC1, from the mitochondrial membrane. Also acts as a regulator of G2 checkpoint and progression to cytokinesis during mitosis.Isoform Bcl-X(L) also regulates presynaptic plasticity, including neurotransmitter release and recovery, number of axonal mitochondria as well as size and number of synaptic vesicle clusters. During synaptic stimulation, increases ATP availability from mitochondria through regulation of mitochondrial membrane ATP synthase F1F0 activity and regulates endocytic vesicle retrieval in hippocampal neurons through association with DMN1L and stimulation of its GTPase activity in synaptic vesicles. May attenuate inflammation impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release (PubMed:17418785).
Research Articles on BCL2L1
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Product Notes
The BCL2L1 bcl2l1 (Catalog #AAA225197) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti Bcl-xL (pSer62) reacts with Human, Mouse (Expected from Sequence), Rat (Expected from Sequence) and may cross-react with other species as described in the data sheet. AAA Biotech's Bcl-xL can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:2000. Researchers should empirically determine the suitability of the BCL2L1 bcl2l1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Bcl-xL, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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