Rabbit Bcl-w Polyclonal Antibody | anti-Bcl-w antibody

Bcl-w (G154) Polyclonal Antibody

Cat. Num. AAA3002738

• Gene Names: BCL2L2; BCLW; BCL-W; PPP1R51; BCL2-L-2
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: Bcl-w; Polyclonal Antibody; Bcl-w (G154) Polyclonal Antibody; Bcl-2-like protein 2; Bcl2-L-2; Apoptosis regulator Bcl-W; BCL2L2; BCLW; KIAA0271; anti-Bcl-w antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Bcl-w (G154) polyclonal antibody detects endogenous levels of Bcl-w protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 193

• Applicable Applications for anti-Bcl-w antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200; IF: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 120-165 of Human Bcl-w.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of Bcl-w (G154) pAb at 1:500 dilutionLane1:C6 whole cell lysate (40ug) Lane2:SP2/0 whole cell lysate (40ug) Lane3:U-87MG whole cell lysate (40ug) Lane4:HEK293T whole cell lysate (40ug))

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of Bcl-w (G154) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.showing cytoplasmic staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.)

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of Bcl-w (G154) pAb in paraffin-embedded human liver carcinoma tissue at 1:50.showing cytoplasmic staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.)

Related Product Information for anti-Bcl-w antibody

Using a PCR-based strategy, an additional protein with life-promoting activity, designated Bcl-w, has been identified. The protein is highly conserved between mouse and human and is encoded by a gene located near the TCRalpha gene on chromosome 14. Bcl-w is expressed in myeloid cell lines but not in T and B lymphocytes, and can be found in a wide range of tissues. An alternative splicing event in exon 4 results in two transcripts. The first, Bcl-w, encodes a protein of 193 amino acids, and the second, Bcl-w/rox, encodes a protein 333 amino acids in length. The "rox" portion of Bcl-w/rox shows a striking 66% amino acid sequence identity with the Drosophila rox2 protein; however, the Bcl-w/rox transcript may be expressed at very low levels.

NCBI and Uniprot Product Information

• NCBI GI #: 315360668
• NCBI GeneID: 599
• NCBI Accession #: NP_001186768.1
• NCBI GenBank Nucleotide #: NP_001186768.1
• UniProt Accession #: Q92843
• Molecular Weight: ~ 21kDa
• NCBI Official Full Name: bcl-2-like protein 2
• NCBI Official Synonym Full Names: BCL2 like 2
• NCBI Official Symbol: BCL2L2
• NCBI Official Synonym Symbols: BCLW; BCL-W; PPP1R51; BCL2-L-2
• NCBI Protein Information: bcl-2-like protein 2
• UniProt Protein Name: Bcl-2-like protein 2
• UniProt Gene Name: BCL2L2
• UniProt Synonym Gene Names: BCLW; KIAA0271; Bcl2-L-2
• UniProt Entry Name: B2CL2_HUMAN

NCBI Description

This gene encodes a member of the BCL-2 protein family. The proteins of this family form hetero- or homodimers and act as anti- and pro-apoptotic regulators. Expression of this gene in cells has been shown to contribute to reduced cell apoptosis under cytotoxic conditions. Studies of the related gene in mice indicated a role in the survival of NGF- and BDNF-dependent neurons. Mutation and knockout studies of the mouse gene demonstrated an essential role in adult spermatogenesis. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the neighboring downstream PABPN1 (poly(A) binding protein, nuclear 1) gene. [provided by RefSeq, Dec 2010]

Uniprot Description

BCL2L2: Promotes cell survival. Blocks dexamethasone-induced apoptosis. Mediates survival of postmitotic Sertoli cells by suppressing death-promoting activity of BAX. Expressed in a wide range of tissues with highest levels in brain, spinal cord, testis, pancreas, heart, spleen and mammary glands. Moderate levels found in thymus, ovary and small intestine. Not detected in salivary gland, muscle or liver. Also expressed in cell lines of myeloid, fibroblast and epithelial origin. Not detected in most lymphoid cell lines. Belongs to the Bcl-2 family. 2 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 14q11.2-q12

Cellular Component: mitochondrial outer membrane; cytosol

Molecular Function: BH domain binding; protein binding; protein homodimerization activity; protein heterodimerization activity

Biological Process: DNA damage response, signal transduction resulting in induction of apoptosis; Sertoli cell proliferation; spermatogenesis; negative regulation of apoptosis