Bcl-W Recombinant Protein | BCL2L2 recombinant protein

Bcl-W Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8119975

• Gene Names: BCL2L2; BCLW; BCL-W; PPP1R51; BCL2-L-2
• Purity: >95% as determined by SDS-PAGE
• Synonyms: Bcl-W; Bcl-W Protein; Human; Recombinant (His Tag); Human BCL-W/BCL2L2 Protein (His Tag); BCL-W Protein; BCL2-L-2 Protein; BCLW Protein; PPP1R51 Protein; BCL2-like 2; BCL2L2 recombinant protein

• Host: E Coli
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 25mM Hepes 0.1MKCl 10% glycerolpH7.5. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Thr172

• Species: Human
• Predicted N Terminal: Met
• Tag: C-His
• Protein Construction: A DNA sequence encoding the human BCL-W (Q92843-1)(Met 1-Thr 172) was fused with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for BCL2L2 recombinant protein

Background: Beta1,4-Galactosyltransferase-I (B4GALT1), one of seven beta1,4-galactosyltransferases, is an enzyme commonly found in the trans-Golgi complex that adds galactose to oligosaccharides. They have an N-terminal hydrophobic signal sequence that directs the protein to the Golgi apparatus and which then remains uncleaved to function as a transmembrane anchor. By sequence similarity, the beta4GalTs form four groups: beta4GalT1 and beta4GalT2, beta4GalT3 and beta4GalT4, beta4GalT5 and beta4GalT6, and beta4GalT7. B4GALT1 gene directs production of B4GALT1 protein using either of two transcription start sites. The product of the smaller transcript serves the traditional biosynthetic role in the Golgi. This form also complexes with alpha-lactalbumin, a mammary-specific protein, to form lactose synthase. In addition to a biosynthetic role, the protein translated from the longer transcript appears on the plasma membranes of some cells where it serves as a signalling receptor in cell-matrix interactions such as sperm-egg binding.

References

Hennet T. (2002) The galactosyltransferase family. Cellular and Molecular Life Sciences. 59(7): 1081-95.Landers EA, et al. (2009) Porcine 1, 4-Galactosyltransferase-I Sequence and Expression. Reproduction in Domestic Animals. 44(2): 228-34.Amado M, et al. (2000) Identification and characterization of large galactosyltransferase gene families: galactosyltransferases for all functions. Biochim Biophys Acta. 1473 (1): 35-53.

NCBI and Uniprot Product Information

• NCBI GI #: 315360668
• NCBI GeneID: 599
• NCBI Accession #: NP_001186768.1
• NCBI GenBank Nucleotide #: NM_001199839.1
• UniProt Accession #: Q92843; Q2M3U0; Q5U0H4; A8K0F4
• Molecular Weight: 20,746 Da
• NCBI Official Full Name: bcl-2-like protein 2
• NCBI Official Synonym Full Names: BCL2-like 2
• NCBI Official Symbol: BCL2L2
• NCBI Official Synonym Symbols: BCLW; BCL-W; PPP1R51; BCL2-L-2
• NCBI Protein Information: bcl-2-like protein 2; apoptosis regulator BCL-W; protein phosphatase 1, regulatory subunit 51
• UniProt Protein Name: Bcl-2-like protein 2
• Protein Family: Bcl-2-like protein
• UniProt Gene Name: BCL2L2
• UniProt Synonym Gene Names: BCLW; KIAA0271; Bcl2-L-2
• UniProt Entry Name: B2CL2_HUMAN

NCBI Description

This gene encodes a member of the BCL-2 protein family. The proteins of this family form hetero- or homodimers and act as anti- and pro-apoptotic regulators. Expression of this gene in cells has been shown to contribute to reduced cell apoptosis under cytotoxic conditions. Studies of the related gene in mice indicated a role in the survival of NGF- and BDNF-dependent neurons. Mutation and knockout studies of the mouse gene demonstrated an essential role in adult spermatogenesis. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the neighboring downstream PABPN1 (poly(A) binding protein, nuclear 1) gene. [provided by RefSeq, Dec 2010]

Uniprot Description

BCL2L2: Promotes cell survival. Blocks dexamethasone-induced apoptosis. Mediates survival of postmitotic Sertoli cells by suppressing death-promoting activity of BAX. Expressed in a wide range of tissues with highest levels in brain, spinal cord, testis, pancreas, heart, spleen and mammary glands. Moderate levels found in thymus, ovary and small intestine. Not detected in salivary gland, muscle or liver. Also expressed in cell lines of myeloid, fibroblast and epithelial origin. Not detected in most lymphoid cell lines. Belongs to the Bcl-2 family. 2 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 14q11.2-q12

Cellular Component: mitochondrial outer membrane; cytosol

Molecular Function: BH domain binding; protein binding; protein homodimerization activity; protein heterodimerization activity

Biological Process: DNA damage response, signal transduction resulting in induction of apoptosis; Sertoli cell proliferation; spermatogenesis; negative regulation of apoptosis