Rabbit Bad Polyclonal Antibody | anti-Bad antibody
Phospho-Bad antibody
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Phospho-Bad antibody was purified by affinity chromatography
Synonyms
Bad; Polyclonal Antibody; Phospho-Bad antibody; Polyclonal Phospho-Bad; anti-Phospho-Bad; phospho BBC2; BCL2L8; BAD; phospho BBC6; Phospho-Ser112; anti-Bad antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Phospho-Bad antibody was purified by affinity chromatography
Form/Format
Supplied in PBS buffer, pH 7.2, containing 50% glycerol, 1% BSA, 0.02% thimerosal
Concentration
200 ug/ml (varies by lot)
Applicable Applications for anti-Bad antibody
Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB)
Application Notes
IP: 5-20 ug/ml
WB: 1-2 ug/ml
WB: 1-2 ug/ml
Biological Significance
Bad is a member of the Bcl-2 family protein that selectively dimerize with Bcl-xL and Bcl-2 resulting in cell death. Survival factors such as IL-3 can inhibit the apoptotic activity of Bad by activating intracellular signaling pathways that result in the phosphorylation of Bad at Ser112 and Ser 136. Phosphorylation at these sites results in the binding of Bad to 14-3-3 proteins and the inhibition of Bad binding to Bcl-xL and Bcl-2. Recently, Akt has also been shown to promote cell survival via its ability to phosphorylate Bad at Ser136.
Cross-Reactivity
Human, Mouse, Rat
Immunogen
Phospho-Bad antibody was raised in Rabbit using synthetic phospho-peptide corresponding to residues surrounding Ser112 of mouse Bad as the immunogen.
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
Related Product Information for anti-Bad antibody
Rabbit polyclonal Phospho-Bad antibody
Product Categories/Family for anti-Bad antibody
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Product Notes
The Bad (Catalog #AAA837381) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Bad can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB). IP: 5-20 ug/ml WB: 1-2 ug/ml. Researchers should empirically determine the suitability of the Bad for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Bad, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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