Western Blot (WB)
(Western blot analysis of phosphorylated Akt in NIH3T3 cells without (left Lane) or with (right Lane) treatment of rapamycin..)
Rabbit Akt Polyclonal Antibody | anti-Akt antibody
Phospho-Akt antibody
Applications
Immunoprecipitation, Western Blot
Purity
Phospho-Akt antibody was purified by affinity chromatography
Synonyms
Akt; Polyclonal Antibody; Phospho-Akt antibody; Polyclonal Phospho-Akt; anti-Phospho-Akt; RAC serine/threonine-protein kinase; Protein kinase B; PKB; DRAC-PK; Dakt1; DAkt; anti-Akt antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Phospho-Akt antibody was purified by affinity chromatography
Form/Format
Supplied in PBS buffer, containing 50% glycerol, 1% BSA, 0.02% thimerosal
Concentration
500 ug/ml (varies by lot)
Sequence Length
286
Applicable Applications for anti-Akt antibody
Immunoprecipitation (IP), Western Blot (WB)
Application Notes
WB: 0.5-4 ug/ml
Biological Significance
Akt, also referred to as PKB and RAC, is the cellular homologue of the viral oncogene v-Akt. This protein kinase is activated by insulin and various growth factors and functions in a pathway involving PI3 kinase as activation is sensitive to wortmanin. Recent evidence suggests that Akt functions to promote cell survival by actively inhibiting apoptosis, although the pathways involved have not been identified. Akt is activated by phospholipid binding and phosphorylation within the activation loop at Thr308 and within the C-terminus at Ser473.
Cross-Reactivity
Human, Mouse, Rat
Immunogen
Phospho-Akt antibody was raised in Rabbit using synthetic phospho-peptide corresponding to residues surrounding Ser473 of mouse Akt as the immunogen.
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
Western Blot (WB)
(Western blot analysis of phosphorylated Akt in NIH3T3 cells without (left Lane) or with (right Lane) treatment of rapamycin..)
Western Blot (WB)
(Western blot analysis of phosphorylated Akt in NIH3T3 cells without (left Lane) or with (right Lane) treatment of rapamycin..)
Related Product Information for anti-Akt antibody
Rabbit polyclonal Phospho-Akt antibody
Product Categories/Family for anti-Akt antibody
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
32,995 Da
NCBI Official Full Name
Akt, partial
UniProt Protein Name
Akt
Protein Family
UniProt Gene Name
Akt
UniProt Entry Name
Q66XT6_ANOST
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Product Notes
The Akt akt (Catalog #AAA837579) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Akt can be used in a range of immunoassay formats including, but not limited to, Immunoprecipitation (IP), Western Blot (WB). WB: 0.5-4 ug/ml. Researchers should empirically determine the suitability of the Akt akt for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Akt, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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