BAD Antibody | anti-BAD antibody

BAD (Phospho-Ser136) Antibody

Cat. Num. AAA856979

• Gene Names: BAD; BBC2; BCL2L8
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry
• Purity: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
• Synonyms: BAD; BAD (Phospho-Ser136) Antibody; anti-BAD antibody

• Reactivity: Human, Mouse, Rat
• Specificity: BAD (phospho-Ser136) antibody detects endogenous levels of BAD only when phosphorylated at Serine 136.
• Purity/Purification: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
• Form/Format: In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Sequence Length: 168

• Applicable Applications for anti-BAD antibody: Immunohistochemistry (IHC)
• Application Notes: IHC: 1:50-1:100
• Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human BAD around the phosphorylation site of serine136 (S-R-SP-A-P).
• Preparation and Storage: Store at-20 degree C for 1 year

Immunohistochemistry (IHC)

(P-Peptide-+ Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using BAD (phospho-Ser136) antibody.)

Product Categories/Family for anti-BAD antibody

Autophagy antibody; Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells

References

Wang XQ, et al. (2001). J Biol Chem.276 (48): 44504-44511. Lee YI, et al. (2001). J Biol Chem.276 (20): 16969-16977. Maiti D, et al. (2001). J Biol Chem.276 (1): 329-333.

NCBI and Uniprot Product Information

• NCBI GI #: 10835069
• NCBI GeneID: 572
• NCBI Accession #: NP_004313.1
• NCBI GenBank Nucleotide #: NM_004322.3
• UniProt Accession #: Q92934; O14803; Q6FH21
• Molecular Weight: 18,392 Da
• NCBI Official Full Name: bcl2-associated agonist of cell death
• NCBI Official Synonym Full Names: BCL2 associated agonist of cell death
• NCBI Official Symbol: BAD
• NCBI Official Synonym Symbols: BBC2; BCL2L8
• NCBI Protein Information: bcl2-associated agonist of cell death
• UniProt Protein Name: Bcl2-associated agonist of cell death
• Protein Family: Bcl2-associated agonist of cell death
• UniProt Gene Name: BAD
• UniProt Synonym Gene Names: BBC6; BCL2L8; BAD; Bcl2-L-8

NCBI Description

The protein encoded by this gene is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin were found to be involved in the regulation of this protein. Alternative splicing of this gene results in two transcript variants which encode the same isoform. [provided by RefSeq, Jul 2008]

Uniprot Description

Promotes cell death. Successfully competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. Can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2 (). Appears to act as a link between growth factor receptor signaling and the apoptotic pathways.