Rabbit AUP1 Polyclonal Antibody | anti-AUP1 antibody

AUP1 Antibody

Cat. Num. AAA9611318

• Reactivity: Human, Mouse, Rat; Predicted: Pig, Bovine, Horse, Sheep, Rabbit, Dog
• Applications: Western Blot
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: AUP1; Polyclonal Antibody; AUP1 Antibody; AA589454; Ancient ubiquitous protein 1; AUP 1; aup1; AUP1_HUMAN; anti-AUP1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat; Predicted: Pig, Bovine, Horse, Sheep, Rabbit, Dog
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: AUP1 Antibody detects endogenous levels of AUP1.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 410

• Applicable Applications for anti-AUP1 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:2000
• Immunogen: A synthesized peptide derived from human AUP1.
• Tissue Specificity: Detected in blood platelets and leukocytes (at protein level). Ubiquitous. Highly expressed in placenta, liver, kidney, skeletal muscle, heart and brain.
• Fragment: Fab Fragment
• Conjugate: Unconjugated
• Subcellular Location: Endoplasmic Reticulum
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts from Jurkat cells, using AUP1 Antibody. The lane on the left was treated with blocking peptide.)

Related Product Information for anti-AUP1 antibody

Description: May play a role in the translocation of terminally misfolded proteins from the endoplasmic reticulum lumen to the cytoplasm and their degradation by the proteasome.

Subunit Structure: Identified in a complex that contains SEL1L, OS9, FAF2/UBXD8, UBE2J1/UBC6E and AUP1. Interacts with the cytoplasmic tail of ITGA2B, ITGA1, ITGA2, ITGA5, ITGAV and ITGAM.

Similarity: Belongs to the AUP1 family.

NCBI and Uniprot Product Information

• NCBI GI #: 31712030
• NCBI GeneID: 550
• NCBI Accession #: NP_853553.1
• NCBI GenBank Nucleotide #: NP_853553.1
• UniProt Accession #: Q9Y679
• Molecular Weight: Observed: 40 kDa; Predicted: 54 kDa
• NCBI Official Full Name: ancient ubiquitous protein 1
• NCBI Official Synonym Full Names: AUP1 lipid droplet regulating VLDL assembly factor
• NCBI Official Symbol: AUP1
• NCBI Protein Information: ancient ubiquitous protein 1
• UniProt Protein Name: Ancient ubiquitous protein 1
• Protein Family: Ancient ubiquitous protein
• UniProt Gene Name: AUP1
• UniProt Entry Name: AUP1_HUMAN

NCBI Description

The protein encoded this gene is involved in several pathways including quality control of misfolded proteins in the endoplasmic reticulum and lipid droplet accumulation. Lipid droplets are organelles in the cytoplasm that store neutral lipids such as cholesterol esters and trigylycerides to prevent the overabundance of free cholesterol and fatty acids in cells, but also to act as storage for other metabolic processes, such as membrane biogenesis. Reduced expression of this gene results in reduced lipid droplet clustering, a function that is dependent on ubiquitination of the protein. This protein contains multiple domains including a hydrophobic N-terminal domain, an acetyltranferase domain, a ubiquitin-binding CUE domain, and a UBE2B2-binding domain (G2BR). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2014]