Western Blot (WB)
(Western blot analysis of FoxO4 in lysates of HuvEc , using FoxO4 Antibody.)
Rabbit FoxO4 Polyclonal Antibody | anti-FoxO4 antibody
FoxO4 Antibody
Predicted: Pig, Bovine, Horse, Sheep, Dog, Chicken, Xenopus
Predicted: Pig, Bovine, Horse, Sheep, Dog, Chicken, Xenopus
ELISA (Peptide): 1:20000-1:40000
Western Blot (WB)
(Western blot analysis of FoxO4 in lysates of HuvEc , using FoxO4 Antibody.)
Western Blot (WB)
(Western blot analysis of FoxO4 in lysates of HuvEc , using FoxO4 Antibody.)
Post Translational Modifications: Acetylation by CREBBP/CBP, which is induced by peroxidase stress, inhibits transcriptional activity. Deacetylation by SIRT1 is NAD-dependent and stimulates transcriptional activity. Phosphorylation by PKB/AKT1 inhibits transcriptional activity and is responsible for cytoplasmic localization. May be phosphorylated at multiple sites by NLK. Monoubiquitinated; monoubiquitination is induced by oxidative stress and reduced by deacetylase inhibitors; results in its relocalization to the nucleus and its increased transcriptional activity. Deubiquitinated by USP7; deubiquitination is induced by oxidative stress; enhances its interaction with USP7 and consequently, deubiquitination; increases its translocation to the cytoplasm and inhibits its transcriptional activity. Hydrogene-peroxide-induced ubiquitination and USP7-mediated deubiquitination have no major effect on its protein stability.
Subunit Structure: Interacts with CREBBP/CBP, CTNNB1, MYOCD, SIRT1, SRF and YWHAZ. Acetylated by CREBBP/CBP and deacetylated by SIRT1. Binding of YWHAZ inhibits DNA-binding. Interacts with USP7; the interaction is enhanced in presence of hydrogen peroxide and occurs independently of TP53. Interacts with NLK, and this inhibits monoubiquitination and transcriptional activity. Interacts with FOXK1; the interaction inhibits MEF2C transactivation activity (By similarity).
NCBI and Uniprot Product Information
Predicted: 54 kDa
NCBI Description
This gene encodes a member of the O class of winged helix/forkhead transcription factor family. Proteins encoded by this class are regulated by factors involved in growth and differentiation indicating they play a role in these processes. A translocation involving this gene on chromosome X and the homolog of the Drosophila trithorax gene, encoding a DNA binding protein, located on chromosome 11 is associated with leukemia. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2010]
Uniprot Description
FOXO4: transcription factor AFX1 containing 1 fork-head domain. May play a role in the insulin signaling pathway. Involved in acute leukemias by a chromosomal translocation t(X;11)(q13;q23) that involves MLLT7 and MLL/HRX. The result is a rogue activator protein. Two splice-variant isoforms have been described.
Protein type: DNA-binding; Oncoprotein; Transcription factor
Chromosomal Location of Human Ortholog: Xq13.1
Cellular Component: nucleoplasm; cytoplasm; cytosol; nucleus
Molecular Function: protein binding; enzyme binding; DNA binding; sequence-specific DNA binding; transcription factor activity; transcription factor binding
Biological Process: epidermal growth factor receptor signaling pathway; transcription from RNA polymerase II promoter; muscle development; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; nerve growth factor receptor signaling pathway; positive regulation of transcription, DNA-dependent; stem cell differentiation; negative regulation of smooth muscle cell differentiation; regulation of transcription from RNA polymerase II promoter; negative regulation of cell proliferation; negative regulation of angiogenesis; regulation of transcription, DNA-dependent; mitotic cell cycle G2/M transition DNA damage checkpoint; insulin receptor signaling pathway; innate immune response; cell cycle arrest
Research Articles on FoxO4
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Product Notes
The FoxO4 foxo4 (Catalog #AAA9611129) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The FoxO4 Antibody reacts with Human, Mouse Predicted: Pig, Bovine, Horse, Sheep, Dog, Chicken, Xenopus and may cross-react with other species as described in the data sheet. AAA Biotech's FoxO4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Peptide ELISA (EIA). WB: 1:500-1:2000 ELISA (Peptide): 1:20000-1:40000. Researchers should empirically determine the suitability of the FoxO4 foxo4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FoxO4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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