Western Blot (WB)
(Western blot analysis of extracts from various samples, using TRIM11 Antibody. Lane 1: 3T3, treated with blocking peptide; Lane 2: 3T3; Lane 3: Hybridoma cells.Observed bands: 45 kDa.)
Rabbit TRIM11 Polyclonal Antibody | anti-TRIM11 antibody
TRIM11 Antibody
Gene Names
TRIM11; BIA1; RNF92
Reactivity
Human, MousePredicted: Pig, Bovine, Sheep, Dog
Applications
Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Synonyms
TRIM11; Polyclonal Antibody; TRIM11 Antibody; BIA 1; BIA1; E3 ubiquitin protein ligase TRIM11; E3 ubiquitin-protein ligase TRIM11; Protein BIA1; RING finger protein 92; RING type E3 ubiquitin transferase TRIM11; RNF 92; RNF92; TRI11_HUMAN; Trim 11; Tripartite motif containing 11; tripartite motif protein 11; Tripartite motif-containing protein 11; anti-TRIM11 antibody
Host
Rabbit
Reactivity
Human, Mouse
Predicted: Pig, Bovine, Sheep, Dog
Predicted: Pig, Bovine, Sheep, Dog
Clonality
Polyclonal
Isotype
IgG
Specificity
TRIM11 Antibody detects endogenous levels of TRIM11.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Form/Format
Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
468
Applicable Applications for anti-TRIM11 antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:2000
Immunogen
A synthesized peptide derived from human TRIM11.
Tissue Specificity
Ubiquitous.
Fragment
Fab Fragment
Conjugate
Unconjugated
Subcellular Location
Nucleus
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of extracts from various samples, using TRIM11 Antibody. Lane 1: 3T3, treated with blocking peptide; Lane 2: 3T3; Lane 3: Hybridoma cells.Observed bands: 45 kDa.)
Western Blot (WB)
(Western blot analysis of extracts from various samples, using TRIM11 Antibody. Lane 1: 3T3, treated with blocking peptide; Lane 2: 3T3; Lane 3: Hybridoma cells.Observed bands: 45 kDa.)
Western Blot (WB)
(Western blot analysis of extracts from K-562 cells, using TRIM11 Antibody. The lane on the left was treated with blocking peptide.)
Western Blot (WB)
(Western blot analysis of extracts from K-562 cells, using TRIM11 Antibody. The lane on the left was treated with blocking peptide.)
Related Product Information for anti-TRIM11 antibody
Description: E3 ubiquitin-protein ligase that promotes the degradation of insoluble ubiquitinated proteins, including insoluble PAX6, poly-Gln repeat expanded HTT and poly-Ala repeat expanded ARX. Mediates PAX6 ubiquitination leading to proteasomal degradation, thereby modulating cortical neurogenesis. May also inhibit PAX6 transcriptional activity, possibly in part by preventing the binding of PAX6 to its consensus sequences. May contribute to the regulation of the intracellular level of HN (humanin) or HN-containing proteins through the proteasomal degradation pathway. Mediates MED15 ubiquitination leading to proteasomal degradation. May contribute to the innate restriction of retroviruses. Upon overexpression, reduces HIV-1 and murine leukemia virus infectivity, by suppressing viral gene expression. Antiviral activity depends on a functional E3 ubiquitin-protein ligase domain. May regulate TRIM5 turnover via the proteasome pathway, thus counteracting the TRIM5-mediated cross-species restriction of retroviral infection at early stages of the retroviral life cycle.
Subunit Structure: Binds cytoplasmic tail of integrin alpha-1. Interacts with the HN peptide (By similarity). Interacts with PHOX2B (By similarity). Interacts with PAX6 (By similarity). Interacts with MED15/ARC105; this interaction leads to MED15 ubiquitination and proteasomal degradation.
Similarity: The coiled-coil domain and the B30.2 domain are both necessary for interaction with HN and PAX6 (By similarity). They are also involved in MED15-binding. The B30.2 domain may be involved cellular protein quality control by promoting the degradation of insoluble ubiquitinated proteins. Belongs to the TRIM/RBCC family.
Subunit Structure: Binds cytoplasmic tail of integrin alpha-1. Interacts with the HN peptide (By similarity). Interacts with PHOX2B (By similarity). Interacts with PAX6 (By similarity). Interacts with MED15/ARC105; this interaction leads to MED15 ubiquitination and proteasomal degradation.
Similarity: The coiled-coil domain and the B30.2 domain are both necessary for interaction with HN and PAX6 (By similarity). They are also involved in MED15-binding. The B30.2 domain may be involved cellular protein quality control by promoting the degradation of insoluble ubiquitinated proteins. Belongs to the TRIM/RBCC family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed: 53 kDa
Predicted: 53 kDa
Predicted: 53 kDa
NCBI Official Full Name
E3 ubiquitin-protein ligase TRIM11
NCBI Official Synonym Full Names
tripartite motif containing 11
NCBI Official Symbol
TRIM11
NCBI Official Synonym Symbols
BIA1; RNF92
NCBI Protein Information
E3 ubiquitin-protein ligase TRIM11
UniProt Protein Name
E3 ubiquitin-protein ligase TRIM11
Protein Family
UniProt Gene Name
TRIM11
UniProt Synonym Gene Names
RNF92
UniProt Entry Name
TRI11_HUMAN
NCBI Description
The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein localizes to the nucleus and the cytoplasm. Its function has not been identified. [provided by RefSeq, Jul 2008]
Research Articles on TRIM11
Similar Products
Product Notes
The TRIM11 trim11 (Catalog #AAA9611531) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TRIM11 Antibody reacts with Human, Mouse Predicted: Pig, Bovine, Sheep, Dog and may cross-react with other species as described in the data sheet. AAA Biotech's TRIM11 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:2000. Researchers should empirically determine the suitability of the TRIM11 trim11 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TRIM11, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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