Rabbit anti-Human, Mouse PTPa Polyclonal Antibody | anti-PTPa antibody

PTPa, NT (Receptor-type Tyrosine-protein Phosphatase alpha, Protein-tyrosine Phosphatase alpha, R-PTP-alpha, PTPRA, PTPRL2) (Biotin)

Cat. Num. AAA6495699

• Gene Names: PTPRA; LRP; HLPR; PTPA; HEPTP; HPTPA; RPTPA; PTPRL2; HPTPalpha; R-PTP-alpha
• Reactivity: Human, Mouse
• Applications: Western Blot, ELISA
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: PTPa; Polyclonal Antibody; NT (Receptor-type Tyrosine-protein Phosphatase alpha; Protein-tyrosine Phosphatase alpha; R-PTP-alpha; PTPRA; PTPRL2) (Biotin); anti-PTPa antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PTPalpha. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-PTPa antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-1:500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-terminal region of human PTPalpha (KLH).
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(The anti-PTPalpha N-term Pab is used in Western blot to detect PTPalpha in mouse brain tissue lysate.)

Product Categories/Family for anti-PTPa antibody

Antibodies; Enzymes; Phosphatase

References

1. Deloukas, P., et al., Nature 414(6866):865-871 (2001). 2. Kaplan, R., et al., Proc. Natl. Acad. Sci. USA 87(18):7000-7004 (1990). 3. Krueger, N.X., et al., EMBO J. 9(10):3241-3252 (1990). 4. Sap, J., et al., Proc. Natl. Acad. Sci. USA 87(16):6112-6116 (1990). 5. Jirik, F.R., et al., FEBS Lett. 273(1-2), 239-242 (1990).

NCBI and Uniprot Product Information

• NCBI GI #: 4506303
• NCBI GeneID: 5786
• NCBI Accession #: NP_002827.1
• NCBI GenBank Nucleotide #: NM_002836.3
• UniProt Accession #: P18433; Q14513; Q7Z2I2; Q96TD9; A8K2G8; D3DVX5
• Molecular Weight: 90,600 Da
• NCBI Official Full Name: receptor-type tyrosine-protein phosphatase alpha isoform 1
• NCBI Official Synonym Full Names: protein tyrosine phosphatase, receptor type, A
• NCBI Official Symbol: PTPRA
• NCBI Official Synonym Symbols: LRP; HLPR; PTPA; HEPTP; HPTPA; RPTPA; PTPRL2; HPTPalpha; R-PTP-alpha
• NCBI Protein Information: receptor-type tyrosine-protein phosphatase alpha; PTPase-alpha; PTPLCA-related phosphatase; tyrosine phosphatase alpha; protein-tyrosine phosphatase alpha; protein tyrosine phosphatase, receptor type, alpha polypeptide; Leukocyte common antigen-related pe
• UniProt Protein Name: Receptor-type tyrosine-protein phosphatase alpha
• Protein Family: Prolyl tripeptidyl peptidase
• UniProt Gene Name: PTPRA
• UniProt Synonym Gene Names: PTPA; PTPRL2; Protein-tyrosine phosphatase alpha; R-PTP-alpha
• UniProt Entry Name: PTPRA_HUMAN

NCBI Description

The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus represents a receptor-type PTP. This PTP has been shown to dephosphorylate and activate Src family tyrosine kinases, and is implicated in the regulation of integrin signaling, cell adhesion and proliferation. Three alternatively spliced variants of this gene, which encode two distinct isoforms, have been reported. [provided by RefSeq, Jul 2008]

Uniprot Description

PTPRA: a receptor protein tyrosine phosphatase (PTP). Contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains. Dephosphorylates and activates Src family tyrosine kinases, and is implicated in the regulation of integrin signaling, cell adhesion and proliferation. Four alternatively spliced isoforms have been reported.

Protein type: Membrane protein, integral; EC 3.1.3.48; Receptor protein phosphatase, tyrosine; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 20p13

Cellular Component: integral to plasma membrane; plasma membrane; receptor complex

Molecular Function: protein binding; transmembrane receptor protein tyrosine phosphatase activity; protein tyrosine phosphatase activity

Biological Process: axon guidance; insulin receptor signaling pathway; protein amino acid phosphorylation