Western Blot (WB)
(Western Blot analysis of MARS2 expression in transfected 293T cell line by MARS2 polyclonal antibody. Lane 1: MARS2 transfected lysate (66.6kD). Lane 2: Non-transfected lysate.)
Rabbit anti-Human MARS2 Polyclonal Antibody | anti-MARS2 antibody
MARS2 (Methionine--tRNA Ligase, Mitochondrial, Methionyl-tRNA Synthetase 2, Mitochondrial Methionyl-tRNA Synthetase, MtMetRS)
Gene Names
MARS2; MetRS; mtMetRS
Reactivity
Human
Applications
Western Blot
Purity
Affinity PurifiedPurified by Protein A affinity chromatography.
Synonyms
MARS2; Polyclonal Antibody; MARS2 (Methionine--tRNA Ligase; Mitochondrial; Methionyl-tRNA Synthetase 2; Mitochondrial Methionyl-tRNA Synthetase; MtMetRS); Anti -MARS2 (Methionine--tRNA Ligase; anti-MARS2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human MARS2.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2.
Sequence
MLRTSVLRLLGRTGASRLSLLEDFGPRYYSSGSLSAGDDACDVRAYFTTPIFYVNAAPHIGHLYSALLADALCRHRRLRGPSTAATRFSTGTDEHGLKIQQAAATAGLAPTELCDRVSEQFQQLFQEAGISCTDFIRTTEARHRVAVQHFWGVLKSRGLLYKGVYEGWYCASDECFLPEAKVTQQPGPSGDSFPVSLESGHPVSWTKEENYIFRLSQFRKPLQRWLRGNPQAITPEPFHHVVLQWLDEELPDLSVSRRSSHLHWGIPVPGDDSQTIYVWLDALVNYLTVIGYPNAEFKSWWPATSHIIGKDILKFHAIYWPAFLLGAGMSPPQRICVHSHWTVCGQKMSKSLGNVVDPRTCLNRYTVDGFRYFLLRQGVPNWDCDYYDEKVVKLLNSELADALGGLLNRCTAKRINPSETYPAFCTTCFPSEPGLVGPSVRAQAEDYALVSAVATLPKQVADHYDNFRIYKALEAVSSCVRQTNGFVQRHAPWKLNWESPVDAPWLGTVLHVALECLRVFGTLLQPVTPSLADKLLSRLGVSASERSLGELYFLPRFYGHPCPFEGRRLGPETGLLFPRLDQSRTWLVKAHRT
Applicable Applications for anti-MARS2 antibody
Western Blot (WB)
Application Notes
Suitable for use in Western Blot.
Immunogen
Full length human MARS2, aa1-593 (NP_612404.1).
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western Blot analysis of MARS2 expression in transfected 293T cell line by MARS2 polyclonal antibody. Lane 1: MARS2 transfected lysate (66.6kD). Lane 2: Non-transfected lysate.)
Western Blot (WB)
(Western Blot analysis of MARS2 expression in transfected 293T cell line by MARS2 polyclonal antibody. Lane 1: MARS2 transfected lysate (66.6kD). Lane 2: Non-transfected lysate.)
Related Product Information for anti-MARS2 antibody
ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met).
Product Categories/Family for anti-MARS2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
66,591 Da
NCBI Official Full Name
methionine--tRNA ligase, mitochondrial
NCBI Official Synonym Full Names
methionyl-tRNA synthetase 2, mitochondrial
NCBI Official Symbol
MARS2
NCBI Official Synonym Symbols
MetRS; mtMetRS
NCBI Protein Information
methionine--tRNA ligase, mitochondrial; methionine-tRNA ligase; methionine--tRNA ligase 2; mitochondrial methionine--tRNA ligase; methionine tRNA ligase 2, mitochondrial; mitochondrial methionyl-tRNA synthetase; methionyl-tRNA synthetase, mitochondrial; methionine-tRNA synthetase 2, mitochondrial; methionine-tRNA synthetase 2 (mitochondrial)
UniProt Protein Name
Methionine--tRNA ligase, mitochondrial
UniProt Gene Name
MARS2
UniProt Synonym Gene Names
MtMetRS
UniProt Entry Name
SYMM_HUMAN
NCBI Description
This gene produces a mitochondrial methionyl-tRNA synthetase protein that is encoded by the nuclear genome and imported to the mitochondrion. This protein likely functions as a monomer and is predicted to localize to the mitochondrial matrix. Mutations in this gene are associated with the autosomal recessive neurodegenerative disease spastic ataxia-3 (SPAX3). [provided by RefSeq, Apr 2014]
Uniprot Description
MARS2: Belongs to the class-I aminoacyl-tRNA synthetase family.
Protein type: EC 6.1.1.10; Ligase; Mitochondrial; Other Amino Acids Metabolism - selenoamino acid
Chromosomal Location of Human Ortholog: 2q33.1
Cellular Component: mitochondrial matrix; cytosol
Molecular Function: methionine-tRNA ligase activity; ATP binding
Biological Process: tRNA aminoacylation for protein translation; methionyl-tRNA aminoacylation; gene expression
Disease: Combined Oxidative Phosphorylation Deficiency 25; Spastic Ataxia 3, Autosomal Recessive
Research Articles on MARS2
Similar Products
Product Notes
The MARS2 mars2 (Catalog #AAA6009762) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MARS2 (Methionine--tRNA Ligase, Mitochondrial, Methionyl-tRNA Synthetase 2, Mitochondrial Methionyl-tRNA Synthetase, MtMetRS) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MARS2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Suitable for use in Western Blot. Researchers should empirically determine the suitability of the MARS2 mars2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MLRTSVLRLL GRTGASRLSL LEDFGPRYYS SGSLSAGDDA CDVRAYFTTP IFYVNAAPHI GHLYSALLAD ALCRHRRLRG PSTAATRFST GTDEHGLKIQ QAAATAGLAP TELCDRVSEQ FQQLFQEAGI SCTDFIRTTE ARHRVAVQHF WGVLKSRGLL YKGVYEGWYC ASDECFLPEA KVTQQPGPSG DSFPVSLESG HPVSWTKEEN YIFRLSQFRK PLQRWLRGNP QAITPEPFHH VVLQWLDEEL PDLSVSRRSS HLHWGIPVPG DDSQTIYVWL DALVNYLTVI GYPNAEFKSW WPATSHIIGK DILKFHAIYW PAFLLGAGMS PPQRICVHSH WTVCGQKMSK SLGNVVDPRT CLNRYTVDGF RYFLLRQGVP NWDCDYYDEK VVKLLNSELA DALGGLLNRC TAKRINPSET YPAFCTTCFP SEPGLVGPSV RAQAEDYALV SAVATLPKQV ADHYDNFRIY KALEAVSSCV RQTNGFVQRH APWKLNWESP VDAPWLGTVL HVALECLRVF GTLLQPVTPS LADKLLSRLG VSASERSLGE LYFLPRFYGH PCPFEGRRLG PETGLLFPRL DQSRTWLVKA HRT. It is sometimes possible for the material contained within the vial of "MARS2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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