Rabbit ATRX Polyclonal Antibody | anti-ATRX antibody

ATRX Polyclonal Antibody

Cat. Num. AAA3007187

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: ATRX; Polyclonal Antibody; ATRX Polyclonal Antibody; Transcriptional regulator ATRX; ATP-dependent helicase ATRX; X-linked helicase II; X-linked nuclear protein; XNP; Znf-HX; RAD54L; XH2; anti-ATRX antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: ATRX pAb detects endogenous levels of ATRX protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 2492

• Applicable Applications for anti-ATRX antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: Synthetic peptide, corresponding Human ATRX.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of ATRX pAb at 1:500 dilutionLane1:3T3-L1 whole cell lysate (40ug) Lane2:C6 whole cell lysate (40ug) Lane3:SGC7901 whole cell lysate (40ug) Lane4:MCF-7 whole cell lysate (40ug) Lane5:EC9706 whole cell lysate (40ug))

Related Product Information for anti-ATRX antibody

ATRX is a member of the Snf2 family of helicase/ATPases, which contribute to the remodeling of the nucelosome structure in an ATP-dependent manner, and facilitate the initiation of transcription and replication. Structurally, ATRX contains a PHD zinc finger motif. ATRX is regulated throughout the cell cycle where it is differentially distributed within the nucleus. During interphase, ATRX predominately associates with the nuclear matrix, while during mitosis, ATRX localizes with condensed chromatin. At the onset of M phase, phosphorylation rapidly induces this redistribution of ATRX to the short arms of human acrocentric chromosomes, where it then specifically complexes with heterochromatin protein 1 alpha to mediate chromosomal segregation. Mutations in the ATRX gene correlate with a high incidence of severe X-linked form of syndromal mental retardation associated with alpha thalassaemia or ATRX syndrome

NCBI and Uniprot Product Information

• NCBI GI #: 20336209
• NCBI Accession #: NP_000480.2
• NCBI GenBank Nucleotide #: NP_000480.2
• UniProt Accession #: P46100
• Molecular Weight: ~ 280kDa
• NCBI Official Full Name: transcriptional regulator ATRX isoform 1
• UniProt Protein Name: Transcriptional regulator ATRX
• Protein Family: Transcriptional regulator
• UniProt Gene Name: ATRX
• UniProt Synonym Gene Names: RAD54L; XH2; XNP
• UniProt Entry Name: ATRX_HUMAN

Uniprot Description

ATRX: a global transcriptional regulator. Belongs to the SNF2 family of proteins, many of which modify gene expression via chromatin remodeling activity. Involved in the developmental silencing of imprinted genes in the brain. Contains one PxVxL motif, which is required for interaction with chromoshadow domains. This motif requires additional residues at -7, -6, +4 and +5 relative to the central V which contact the chromoshadow domain. Constitutive mutations in ATRX are associated with brain, facial, and genital abnormalities, and alpha thalassemia. Acquired mutations in ATRX have been observed in preleukemic conditions. Six alternatively spliced human isoforms have been described.

Protein type: DNA repair, damage; Ubiquitin conjugating system; EC 3.6.4.12; Helicase

Chromosomal Location of Human Ortholog: Xq21.1

Cellular Component: nucleoplasm; PML body; nuclear heterochromatin; nucleus

Molecular Function: protein binding; DNA helicase activity; DNA binding; histone binding; zinc ion binding; chromatin binding; helicase activity; DNA translocase activity; methylated histone residue binding; ATP binding

Biological Process: transcription, DNA-dependent; DNA damage response, signal transduction by p53 class mediator; positive regulation of telomere maintenance; DNA repair; Sertoli cell development; DNA duplex unwinding; replication fork processing; DNA recombination; chromatin remodeling; nucleosome assembly; DNA replication-independent nucleosome assembly; regulation of transcription, DNA-dependent; forebrain development; DNA methylation; spermatogenesis; positive regulation of transcription from RNA polymerase II promoter

Disease: Alpha-thalassemia/mental Retardation Syndrome, X-linked; Mental Retardation-hypotonic Facies Syndrome, X-linked, 1; Alpha-thalassemia Myelodysplasia Syndrome