Mouse anti-Human RAD54 / ATRX Monoclonal Antibody | anti-RAD54 antibody

Mouse Monoclonal [clone 60.1] (IgG) to Human RAD54 / ATRX

Cat. Num. AAA245065

• Gene Names: ATRX; JMS; SHS; XH2; XNP; ATR2; SFM1; RAD54; MRXHF1; RAD54L; ZNF-HX
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Protein G Purified
• Synonyms: RAD54 / ATRX; Monoclonal Antibody; Mouse Monoclonal [clone 60.1] (IgG) to Human RAD54 / ATRX; Anti-RAD54 / ATRX Antibody (C-Terminus; clone 60.1) IHC-plus; ATRX; ATP-dependent helicase ATRX; ATR2; Juberg-Marsidi syndrome; JMS; RAD54; RAD54 homolog; SFM1; MRXHF1; XH2; XNP; Transcriptional regulator ATRX; ZNF-HX; X-linked nuclear protein; SHS; Helicase 2; X-linked; X-linked helicase II; Zinc finger helicase; Human RAD54; anti-RAD54 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG
• Clone Number: 60.1
• Specificity: Peptides from N and C termini of RAD54L (ATRX)
• Purity/Purification: Protein G Purified
• Form/Format: PBS, pH 7.2. No preservatives added.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 2492

• Applicable Applications for anti-RAD54 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA, IHC-P (5 ug/ml), WB; Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...
• Target Species: Human
• Immunogen Description: Synthetic peptide from human ATRX.
• Immunogen Type: Synthetic peptide
• Immunogen: RAD54 / ATRX antibody was raised against synthetic peptide from human ATRX.
• Antigen Modification: C-Terminus
• Preparation and Storage: Long term: -20 degree C; Short term: +4 degree C. Avoid repeat freeze-thaw cycles.

Immunohistochemistry (IHC)

(Anti-ATRX antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Related Product Information for anti-RAD54 antibody

The protein encoded by this gene contains an ATPase/helicase domain, and thus it belongs to the SWI/SNF family of chromatin remodeling proteins. The mutations of this gene are associated with an X-linked mental retardation (XLMR) syndrome most often accompanied by alpha-thalassemia (ATRX) syndrome.

NCBI and Uniprot Product Information

• NCBI GI #: 20336209
• NCBI GeneID: 546
• NCBI Accession #: NP_000480.2
• NCBI GenBank Nucleotide #: NM_000489.3
• UniProt Accession #: P46100; P51068; Q15886; Q59FB5; Q59H31; Q5H9A2; Q5JWI4; Q7Z2J1; Q9H0Z1; Q9NTS3; D3DTE2
• Molecular Weight: 151,556 Da
• NCBI Official Full Name: transcriptional regulator ATRX isoform 1
• NCBI Official Synonym Full Names: alpha thalassemia/mental retardation syndrome X-linked
• NCBI Official Symbol: ATRX
• NCBI Official Synonym Symbols: JMS; SHS; XH2; XNP; ATR2; SFM1; RAD54; MRXHF1; RAD54L; ZNF-HX
• NCBI Protein Information: transcriptional regulator ATRX; X-linked helicase II; Zinc finger helicase; helicase 2, X-linked; X-linked nuclear protein; ATP-dependent helicase ATRX; DNA dependent ATPase and helicase; alpha thalassemia/mental retardation syndrome X-linked (RAD54 homolog, S. cerevisiae)
• UniProt Protein Name: Transcriptional regulator ATRX
• Protein Family: DNA repair and recombination protein
• UniProt Gene Name: ATRX
• UniProt Synonym Gene Names: RAD54L; XH2; XNP
• UniProt Entry Name: ATRX_HUMAN

NCBI Description

The protein encoded by this gene contains an ATPase/helicase domain, and thus it belongs to the SWI/SNF family of chromatin remodeling proteins. This protein is found to undergo cell cycle-dependent phosphorylation, which regulates its nuclear matrix and chromatin association, and suggests its involvement in the gene regulation at interphase and chromosomal segregation in mitosis. Mutations in this gene are associated with an X-linked mental retardation (XLMR) syndrome most often accompanied by alpha-thalassemia (ATRX) syndrome. These mutations have been shown to cause diverse changes in the pattern of DNA methylation, which may provide a link between chromatin remodeling, DNA methylation, and gene expression in developmental processes. Multiple alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Aug 2013]

Uniprot Description

ATRX: a global transcriptional regulator. Belongs to the SNF2 family of proteins, many of which modify gene expression via chromatin remodeling activity. Involved in the developmental silencing of imprinted genes in the brain. Contains one PxVxL motif, which is required for interaction with chromoshadow domains. This motif requires additional residues at -7, -6, +4 and +5 relative to the central V which contact the chromoshadow domain. Constitutive mutations in ATRX are associated with brain, facial, and genital abnormalities, and alpha thalassemia. Acquired mutations in ATRX have been observed in preleukemic conditions. Six alternatively spliced human isoforms have been described.

Protein type: EC 3.6.4.12; Helicase; Ubiquitin conjugating system; DNA repair, damage

Chromosomal Location of Human Ortholog: Xq21.1

Cellular Component: nucleoplasm; PML body; nuclear heterochromatin; nucleus

Molecular Function: DNA helicase activity; protein binding; DNA binding; zinc ion binding; histone binding; chromatin binding; helicase activity; DNA translocase activity; ATP binding; methylated histone residue binding

Biological Process: DNA damage response, signal transduction by p53 class mediator; transcription, DNA-dependent; positive regulation of telomere maintenance; DNA repair; Sertoli cell development; DNA duplex unwinding; replication fork processing; DNA recombination; chromatin remodeling; nucleosome assembly; regulation of transcription, DNA-dependent; DNA replication-independent nucleosome assembly; forebrain development; DNA methylation; positive regulation of transcription from RNA polymerase II promoter; spermatogenesis

Disease: Alpha-thalassemia/mental Retardation Syndrome, X-linked; Mental Retardation-hypotonic Facies Syndrome, X-linked, 1; Alpha-thalassemia Myelodysplasia Syndrome