Rabbit anti-Human HRH1 Polyclonal Antibody | anti-HRH1 antibody

HRH1, ID (HRH1, Histamine H1 receptor) (FITC)

Cat. Num. AAA6308378

• Gene Names: HRH1; H1-R; hisH1
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: HRH1; Polyclonal Antibody; ID (HRH1; Histamine H1 receptor) (FITC); Histamine H1 receptor; anti-HRH1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-HRH1 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: HRH1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 272-301 amino acids from the Central region of human HRH1.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(HRH1 Antibody (Center) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the HRH1 antibody detected the HRH1 protein (arrow).)

Immunohistochemistry (IHC)

(HRH1 Antibody (Center) (AP14425c)immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of HRH1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-HRH1 antibody

Histamine is a ubiquitous messenger molecule released from mast cells, enterochromaffin-like cells, and neurons. Its various actions are mediated by histamine receptors H1, H2, H3 and H4. This gene was thought to be intronless until recently. The protein encoded by this gene is an integral membrane protein and belongs to the G protein-coupled receptor superfamily. It mediates the contraction of smooth muscles, the increase in capillary permeability due to contraction of terminal venules, the release of catecholamine from adrenal medulla, and neurotransmission in the central nervous system. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq].

Product Categories/Family for anti-HRH1 antibody

Antibodies; Receptors

References

Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). Ruano, G., et al. Pharmacogenomics 11(7):959-971(2010). Davila, S., et al. Genes Immun. 11(3):232-238(2010). Notcovich, C., et al. Exp. Cell Res. 316(3):401-411(2010). Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 4504491
• NCBI GeneID: 3269
• NCBI Accession #: NP_000852.1; NP_001091682.1
• NCBI GenBank Nucleotide #: NM_000861.3
• UniProt Accession #: P35367; Q6P9E5; A8K047
• Molecular Weight: 55,784 Da
• NCBI Official Full Name: histamine H1 receptor
• NCBI Official Synonym Full Names: histamine receptor H1
• NCBI Official Symbol: HRH1
• NCBI Official Synonym Symbols: H1-R; hisH1
• NCBI Protein Information: histamine H1 receptor; H1R; HH1R; histamine receptor, subclass H1
• UniProt Protein Name: Histamine H1 receptor
• Protein Family: Histamine H1 receptor
• UniProt Gene Name: HRH1
• UniProt Synonym Gene Names: H1R; HH1R
• UniProt Entry Name: HRH1_HUMAN

NCBI Description

Histamine is a ubiquitous messenger molecule released from mast cells, enterochromaffin-like cells, and neurons. Its various actions are mediated by histamine receptors H1, H2, H3 and H4. This gene was thought to be intronless until recently. The protein encoded by this gene is an integral membrane protein and belongs to the G protein-coupled receptor superfamily. It mediates the contraction of smooth muscles, the increase in capillary permeability due to contraction of terminal venules, the release of catecholamine from adrenal medulla, and neurotransmission in the central nervous system. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

H1R: In peripheral tissues, the H1 subclass of histamine receptors mediates the contraction of smooth muscles, increase in capillary permeability due to contraction of terminal venules, and catecholamine release from adrenal medulla, as well as mediating neurotransmission in the central nervous system. Belongs to the G-protein coupled receptor 1 family.

Protein type: Membrane protein, integral; Receptor, GPCR; GPCR, family 1; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 3p25

Cellular Component: nucleoplasm; integral to plasma membrane; cytoplasm; plasma membrane

Molecular Function: G-protein coupled receptor activity; histamine receptor activity

Biological Process: G-protein coupled receptor protein signaling pathway; inositol phosphate-mediated signaling; positive regulation of vasoconstriction; eosinophil chemotaxis; regulation of synaptic plasticity; visual learning; positive regulation of inositol trisphosphate biosynthetic process; G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); regulation of vascular permeability; inflammatory response; memory