Rabbit Fbx32 Polyclonal Antibody | anti-Fbx32 antibody

Fbx32 Polyclonal Antibody

Cat. Num. AAA3012681

• Gene Names: FBXO32; Fbx32; MAFbx
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunocytochemistry
• Purity: ProA affinity purified
• Synonyms: Fbx32; Polyclonal Antibody; Fbx32 Polyclonal Antibody; MAFbx; FBXO32; 4833442G10Rik; AI430017; Atrogin 1; Atrogin-1; ATROGIN1; Atrophy gene 1; F box only protein 32; F-box only protein 32; F-box protein 32; FBX32; fbxo25; FLJ32424; MGC108443; MGC137646; MGC33610; Muscle atrophy F box; Muscle atrophy F box protein; Muscle atrophy F-box protein; anti-Fbx32 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Fbx32 polyclonal antibody detects endogenous levels of Fbx32 protein.
• Purity/Purification: ProA affinity purified
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 355

• Applicable Applications for anti-Fbx32 antibody: Western Blot (WB), Immunocytochemistry (ICC)
• Application Notes: WB: 1:500-1:1,000; ICC: 1:50-1:200
• Immunogen: Recombinant protein corresponding to human Fbx32 1-200aa.
• Preparation and Storage: Store at 4 degree C after thawing.; Aliquot store at -20 degree C.; Avoid repeated freeze/thaw cycles.

Western Blot (WB)

(Western Blot analysis of Fbx32 on mouse skeletal muscle tissue using anti-Fbx32 antibody at 1/1,000 dilution.)

Immunocytochemistry (ICC)

(ICC staining Fbx32 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

Related Product Information for anti-Fbx32 antibody

Muscle atrophy F-box (MAFbx) is an E3 ubiquitin ligase that initiates ATP-dependent ubiquitin-mediated proteolysis and promotes muscle atrophy. MAFbx transcript is abundant in cardiac and skeletal muscle undergoing atrophy. MAFbx -/- mice are resistant to muscle atrophy. MAFbx is thought to recognize and bind to some phosphorylated proteins and promote their ubiquitination and degradation during skeletal muscle atrophy. It interacts with MyoD by ubiquitination via a sequence found in transcriptional coactivators and therefore may play an important role in the course of muscle differentiation by determining the abundance of MyoD. MAFbx is specifically expressed in cardiac and skeletal muscle.

NCBI and Uniprot Product Information

• NCBI GI #: 20177894
• NCBI GeneID: 114907
• UniProt Accession #: Q969P5; Q9CPU7; Q91Z62
• Molecular Weight: 41kDa
• NCBI Official Full Name: F-box only protein 32
• NCBI Official Synonym Full Names: F-box protein 32
• NCBI Official Symbol: FBXO32
• NCBI Official Synonym Symbols: Fbx32; MAFbx
• NCBI Protein Information: F-box only protein 32
• UniProt Protein Name: F-box only protein 32
• UniProt Gene Name: FBXO32
• UniProt Synonym Gene Names: MAFbx
• UniProt Entry Name: FBX32_HUMAN

NCBI Description

This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class and contains an F-box domain. This protein is highly expressed during muscle atrophy, whereas mice deficient in this gene were found to be resistant to atrophy. This protein is thus a potential drug target for the treatment of muscle atrophy. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jun 2011]

Uniprot Description

FBXO32: Substrate recognition component of a (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Probably recognizes and binds to phosphorylated target proteins during skeletal muscle atrophy. Recognizes TERF1. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 8q24.13

Cellular Component: nucleolus; Z disc

Molecular Function: protein binding

Biological Process: protein ubiquitination; response to denervation involved in regulation of muscle adaptation