Rabbit anti-Human GBRR3 Polyclonal Antibody | anti-GBRR3 antibody

GBRR3, NT (GABRR3, Gamma-aminobutyric acid receptor subunit rho-3, GABA(A) receptor subunit rho-3, GABA(C) receptor) (Azide free) (HRP)

Cat. Num. AAA6301515

• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: GBRR3; Polyclonal Antibody; NT (GABRR3; Gamma-aminobutyric acid receptor subunit rho-3; GABA(A) receptor subunit rho-3; GABA(C) receptor) (Azide free) (HRP); GABRR3; GABA(C) receptor; anti-GBRR3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-GBRR3 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: GBRR3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 42-71 amino acids from the N-terminal region of human GBRR3.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(GBRR3 Antibody (N-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the GBRR3 antibody detected the GBRR3 protein (arrow).)

Related Product Information for anti-GBRR3 antibody

GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel (By similarity).

Product Categories/Family for anti-GBRR3 antibody

Antibodies; Receptors

References

Craddock, N., et al. Mol. Psychiatry 15(2):146-153(2010). Gratacos, M., et al. Am. J. Med. Genet. B Neuropsychiatr. Genet. 150B (6), 808-816 (2009): Muzny, D.M., et al. Nature 440(7088):1194-1198(2006). Alakuijala, A., et al. Brain Res. Dev. Brain Res. 154(1):15-23(2005). Croci, C., et al. J. Biol. Chem. 278(8):6128-6135(2003).

NCBI and Uniprot Product Information

• NCBI GI #: 157743288
• NCBI GeneID: 200959
• NCBI Accession #: NP_001099050.1
• NCBI GenBank Nucleotide #: NM_001105580.2
• UniProt Accession #: A8MPY1; Q9UIV9
• Molecular Weight: 54,272 Da
• NCBI Official Full Name: gamma-aminobutyric acid receptor subunit rho-3
• NCBI Official Synonym Full Names: gamma-aminobutyric acid (GABA) A receptor, rho 3 (gene/pseudogene)
• NCBI Official Symbol: GABRR3
• NCBI Protein Information: gamma-aminobutyric acid receptor subunit rho-3
• UniProt Protein Name: Gamma-aminobutyric acid receptor subunit rho-3
• UniProt Gene Name: GABRR3
• UniProt Entry Name: GBRR3_HUMAN

NCBI Description

The neurotransmitter gamma-aminobutyric acid (GABA) functions in the central nervous system to regulate synaptic transmission of neurons. This gene encodes one of three related subunits, which combine as homo- or hetero-pentamers to form GABA(C) receptors. In humans, some individuals contain a single-base polymorphism (dbSNP rs832032) that is predicted to inactivate the gene product. [provided by RefSeq, Jan 2012]

Uniprot Description

GABRR3: GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel. Belongs to the ligand-gated ion channel (TC 1.A.9) family. Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily. GABRR3 sub-subfamily.

Protein type: Transporter; Membrane protein, multi-pass; Channel, ligand-gated; Membrane protein, integral; Transporter, ion channel

Chromosomal Location of Human Ortholog: 3q11.2

Cellular Component: postsynaptic membrane; plasma membrane; cell junction

Molecular Function: protein domain specific binding; chloride channel activity; GABA-A receptor activity; extracellular ligand-gated ion channel activity

Biological Process: synaptic transmission; transmembrane transport; gamma-aminobutyric acid signaling pathway