Rabbit anti-Human ATP6V1G1 Polyclonal Antibody | anti-ATP6V1G1 antibody

ATP6V1G1, CT (ATP6V1G1, ATP6G, ATP6G1, ATP6J, V-type proton ATPase subunit G 1, V-ATPase 13kD subunit 1, Vacuolar proton pump subunit G 1, Vacuolar proton pump subunit M16) (Azide free) (HRP)

Cat. Num. AAA6276715

• Gene Names: ATP6V1G1; ATP6G; ATP6J; Vma10; ATP6G1; ATP6GL
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: ATP6V1G1; Polyclonal Antibody; CT (ATP6V1G1; ATP6G; ATP6G1; ATP6J; V-type proton ATPase subunit G 1; V-ATPase 13kD subunit 1; Vacuolar proton pump subunit G 1; Vacuolar proton pump subunit M16) (Azide free) (HRP); Vacuolar proton pump subunit M16; anti-ATP6V1G1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-ATP6V1G1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: ATP6V1G1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 90-118 amino acids from the C-terminal region of human ATP6V1G1.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(ATP6V1G1 Antibody (C-term) western blot analysis in ZR-75-1 cell line lysates (35ug/lane).This demonstrates the ATP6V1G1 antibody detected the ATP6V1G1 protein (arrow).)

Related Product Information for anti-ATP6V1G1 antibody

This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A, three B, and two G subunits, as well as a C, D, E, F, and H subunit. The V1 domain contains the ATP catalytic site. The protein encoded by this gene is one of three V1 domain G subunit proteins. Pseudogenes of this gene have been characterized.

Product Categories/Family for anti-ATP6V1G1 antibody

Antibodies; Enzymes; ATPase

References

Norgett, E.E., et al. J. Biol. Chem. 282(19):14421-14427(2007). Lamesch, P., et al. Genomics 89(3):307-315(2007). Stelzl, U., et al. Cell 122(6):957-968(2005). Morel, N. Biol. Cell 95(7):453-457(2003). Smith, A.N., et al. Mol. Cell 12(4):801-803(2003).

NCBI and Uniprot Product Information

• NCBI GI #: 4757818
• NCBI GeneID: 9550
• NCBI Accession #: NP_004879.1
• NCBI GenBank Nucleotide #: NM_004888.3
• UniProt Accession #: O75348; Q6IB33
• Molecular Weight: 13,758 Da
• NCBI Official Full Name: V-type proton ATPase subunit G 1
• NCBI Official Synonym Full Names: ATPase, H+ transporting, lysosomal 13kDa, V1 subunit G1
• NCBI Official Symbol: ATP6V1G1
• NCBI Official Synonym Symbols: ATP6G; ATP6J; Vma10; ATP6G1; ATP6GL
• NCBI Protein Information: V-type proton ATPase subunit G 1; V-ATPase subunit G 1; V-ATPase 13 kDa subunit 1; vacuolar H(+)-ATPase subunit G 1; vacuolar proton pump subunit G 1; vacuolar proton pump subunit M16; vacuolar ATP synthase subunit M16; ATPase, H+ transporting, lysosomal
• UniProt Protein Name: V-type proton ATPase subunit G 1
• Protein Family: V-type proton ATPase
• UniProt Gene Name: ATP6V1G1
• UniProt Synonym Gene Names: ATP6G; ATP6G1; ATP6J; V-ATPase subunit G 1
• UniProt Entry Name: VATG1_HUMAN

Uniprot Description

ATP6V1G1: Catalytic subunit of the peripheral V1 complex of vacuolar ATPase (V-ATPase). V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells. Belongs to the V-ATPase G subunit family.

Protein type: Vesicle; Hydrolase

Chromosomal Location of Human Ortholog: 9q32

Cellular Component: lysosomal membrane; plasma membrane; vacuolar proton-transporting V-type ATPase complex; cytosol

Molecular Function: hydrogen-exporting ATPase activity, phosphorylative mechanism; ATPase binding

Biological Process: interaction with host; cellular iron ion homeostasis; metabolic process; insulin receptor signaling pathway; transferrin transport; transmembrane transport