Rabbit anti-Human ATP6V1G1 Polyclonal Antibody | anti-ATP6V1G1 antibody

ATP6V1G1, CT (ATP6V1G1, ATP6G, ATP6G1, ATP6J, V-type proton ATPase subunit G 1, V-ATPase 13kD subunit 1, Vacuolar proton pump subunit G 1, Vacuolar proton pump subunit M16) (APC)

Cat. Num. AAA6276712

• Gene Names: ATP6V1G1; ATP6G; ATP6J; Vma10; ATP6G1; ATP6GL
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: ATP6V1G1; Polyclonal Antibody; CT (ATP6V1G1; ATP6G; ATP6G1; ATP6J; V-type proton ATPase subunit G 1; V-ATPase 13kD subunit 1; Vacuolar proton pump subunit G 1; Vacuolar proton pump subunit M16) (APC); Vacuolar proton pump subunit M16; anti-ATP6V1G1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-ATP6V1G1 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: ATP6V1G1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 90-118 amino acids from the C-terminal region of human ATP6V1G1.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(ATP6V1G1 Antibody (C-term) western blot analysis in ZR-75-1 cell line lysates (35ug/lane).This demonstrates the ATP6V1G1 antibody detected the ATP6V1G1 protein (arrow).)

Related Product Information for anti-ATP6V1G1 antibody

This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A, three B, and two G subunits, as well as a C, D, E, F, and H subunit. The V1 domain contains the ATP catalytic site. The protein encoded by this gene is one of three V1 domain G subunit proteins. Pseudogenes of this gene have been characterized.

Product Categories/Family for anti-ATP6V1G1 antibody

Antibodies; Enzymes; ATPase

References

Norgett, E.E., et al. J. Biol. Chem. 282(19):14421-14427(2007). Lamesch, P., et al. Genomics 89(3):307-315(2007). Stelzl, U., et al. Cell 122(6):957-968(2005). Morel, N. Biol. Cell 95(7):453-457(2003). Smith, A.N., et al. Mol. Cell 12(4):801-803(2003).

NCBI and Uniprot Product Information

• NCBI GI #: 4757818
• NCBI GeneID: 9550
• NCBI Accession #: NP_004879.1
• NCBI GenBank Nucleotide #: NM_004888.3
• UniProt Accession #: O75348; Q6IB33
• Molecular Weight: 13,758 Da
• NCBI Official Full Name: V-type proton ATPase subunit G 1
• NCBI Official Synonym Full Names: ATPase, H+ transporting, lysosomal 13kDa, V1 subunit G1
• NCBI Official Symbol: ATP6V1G1
• NCBI Official Synonym Symbols: ATP6G; ATP6J; Vma10; ATP6G1; ATP6GL
• NCBI Protein Information: V-type proton ATPase subunit G 1; V-ATPase subunit G 1; V-ATPase 13 kDa subunit 1; vacuolar H(+)-ATPase subunit G 1; vacuolar proton pump subunit G 1; vacuolar proton pump subunit M16; vacuolar ATP synthase subunit M16; ATPase, H+ transporting, lysosomal
• UniProt Protein Name: V-type proton ATPase subunit G 1
• Protein Family: V-type proton ATPase
• UniProt Gene Name: ATP6V1G1
• UniProt Synonym Gene Names: ATP6G; ATP6G1; ATP6J; V-ATPase subunit G 1
• UniProt Entry Name: VATG1_HUMAN

Uniprot Description

ATP6V1G1: Catalytic subunit of the peripheral V1 complex of vacuolar ATPase (V-ATPase). V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells. Belongs to the V-ATPase G subunit family.

Protein type: Vesicle; Hydrolase

Chromosomal Location of Human Ortholog: 9q32

Cellular Component: lysosomal membrane; plasma membrane; vacuolar proton-transporting V-type ATPase complex; cytosol

Molecular Function: hydrogen-exporting ATPase activity, phosphorylative mechanism; ATPase binding

Biological Process: interaction with host; cellular iron ion homeostasis; metabolic process; insulin receptor signaling pathway; transferrin transport; transmembrane transport