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Western Blot (WB) (Western Blot: Sample: Recombinant MMP2, Porcine.)

Rabbit anti-Pig Matrix Metalloproteinase 2 (MMP2) Polyclonal Antibody | anti-MMP2 antibody

Polyclonal Antibody to Matrix Metalloproteinase 2 (MMP2)

Gene Names
Mmp2; 2-MMP; anon-WO0118547.84; CG1794; dm-2MMP; Dm2-MMP; DmelCG1794; dmmp2; l(2)02353; mmp2; MMP2
Reactivity
Pig
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation
Purity
Antigen-specific affinity chromatography
Synonyms
Matrix Metalloproteinase 2 (MMP2); Polyclonal Antibody; Polyclonal Antibody to Matrix Metalloproteinase 2 (MMP2); CLG4; CLG4A; MMP-II; MONA; TBE-1; PEX; Gelatinase A; 72kDa Gelatinase; 72kDa Type IV Collagenase; anti-MMP2 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Pig
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against MMP2. It has been selected for its ability to recognize MMP2 in immunohistochemical staining and western blotting.
Purity/Purification
Antigen-specific affinity chromatography
Form/Format
Liquid
Concentration
500ug/mL (varies by lot)
Applicable Applications for anti-MMP2 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)
Application Notes
Western Blot: 0.5-2ug/ml
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Immunogen
Recombinant Matrix Metalloproteinase 2 (MMP2)
Cross Reactivity
Pig
Buffer Formulation
PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Label
None
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2037762
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Western Blot (WB)

(Western Blot: Sample: Recombinant MMP2, Porcine.)

Western Blot (WB) (Western Blot: Sample: Recombinant MMP2, Porcine.)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
77,261 Da
NCBI Official Full Name
matrix metalloproteinase 2, isoform D
NCBI Official Synonym Full Names
Matrix metalloproteinase 2
NCBI Official Symbol
Mmp2
NCBI Official Synonym Symbols
2-MMP; anon-WO0118547.84; CG1794; dm-2MMP; Dm2-MMP; DmelCG1794; dmmp2; l(2)02353; mmp2; MMP2
NCBI Protein Information
CG1794 gene product from transcript CG1794-RB
UniProt Protein Name
Matrix metalloproteinase-2
Protein Family
UniProt Gene Name
Mmp2
UniProt Synonym Gene Names
Dm2-MMP

Uniprot Description

Has metalloproteinase activity (PubMed:11967260). Required for larval tissue histolysis during metamorphosis and is involved in pupal head eversion and fusion of the wing imaginal tissue (PubMed:12530966). Required for growth of the dorsal air sac primordium and development of the dorsal air sacs (PubMed:19751719). Promotes embryonic motor axon fasciculation (PubMed:18045838). Cleaves and activates frac to promote motor axon bundling during outgrowth (PubMed:21471368). Promotes the reshaping of adult sensory neuron dendrites from a radial to lattice-like shape which occurs after eclosion by degrading the basement membrane on which the dendrites grow (PubMed:20412776). Involved in inhibition of follicle stem cell proliferation by cleaving Dlp, inhibiting its interaction with wg and preventing Dlp-mediated spreading of wg to follicle stem cells to enhance their proliferation (PubMed:25267296). Plays a role in wound healing (PubMed:22262460). Involved in fat body dissociation which occurs during metamorphosis by degrading basement membrane components, leading to destruction of cell-basement membrane junctions (PubMed:25520167). Required for posterior follicle cell degradation and ovulation (PubMed:25695427).

Research Articles on MMP2

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Product Notes

The MMP2 mmp2 (Catalog #AAA2085966) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Matrix Metalloproteinase 2 (MMP2) reacts with Pig and may cross-react with other species as described in the data sheet. AAA Biotech's Matrix Metalloproteinase 2 (MMP2) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP). Western Blot: 0.5-2ug/ml Immunocytochemistry in formalin fixed cells: 5-20ug/ml Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml Immunohistochemistry in paraffin section: 5-20ug/ml Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml. Researchers should empirically determine the suitability of the MMP2 mmp2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Matrix Metalloproteinase 2 (MMP2), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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