Rabbit anti-Human PHPT1 Monoclonal Antibody | anti-PHPT1 antibody

Recombinant Anti-PHPT1 Antibody, Rabbit Monoclonal

Cat. Num. AAA8106462

• Gene Names: PHPT1; PHP14; CGI-202; HSPC141; HEL-S-132P
• Reactivity: Human
• Applications: Immunohistochemistry, Flow Cytometry, Functional Assay, Immunocytochemistry, Immunofluorescence
• Purity: Protein A
• Synonyms: PHPT1; Monoclonal Antibody; Recombinant Anti-PHPT1 Antibody; Rabbit Monoclonal; PHPT1 Antibody; Rabbit MAb; phosphohistidine phosphatase 1; Anti-CGI-202 Antibody; Anti-HEL-S-132P Antibody; Anti-HSPC141 Antibody; Anti-PHP14 Antibody; anti-PHPT1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Rabbit IgG
• Clone Number: 001
• Specificity: Human PHPT1
• Purity/Purification: Protein A
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-PHPT1 antibody: Immunohistochemistry-Paraffin (IHC-P), Flow Cytometry (FC/FACS/FCM), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: IHC-P: 1:2500-1:10,000; ICC/IF: 1:20-1:100; FC/FACS/FCM: 1:25-1:100
• Antibody Type: Recombinant Antibody
• Immunogen: Recombinant Human PHPT1 protein
• Conjugation: Unconjugated
• Preparation: This antibody was obtained from a rabbit immunized with purified, recombinant Human PHPT1 (rh PHPT1).
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunochemical staining of human PHPT1 in human skeletal muscle with rabbit monoclonal antibody (1:5000, formalin-fixed paraffin embedded sections).)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of Human PHPT1 expression on THP1 cells. The cells were treated according to manufacturer's manual (BD Pharmingen'), stained with purified anti-Human PHPT1, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.)

Immunofluorescence (IF)

(Immunofluorescence staining of Human PHPT1 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human PHPT1 monoclonal antibody (1:60) at 37 degree C 1 hour. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.)

Related Product Information for anti-PHPT1 antibody

PHPT1, also known as 14 kDa phosphohistidine phosphatase, phosphohistidine phosphatase 1, protein janus-A homolog, PHP14, is a cytoplasm protein which belongs to the janus family. PHPT1/PHP14 is expressed abundantly in heart and skeletal muscle. Phosphatases are a diverse group of enzymes that regulate numerous cellular processes. Much of what is known relates to the tyrosine, threonine, and serine phosphatases, whereas the histidine phosphatases have not been studied as much. Protein histidine phosphorylation exists widely in vertebrates, and it plays important roles in signal transduction and other cellular functions. Protein histidine phosphorylation accounts for about 6% of the total protein phosphorylation in eukaryotic cells. The knowledge about eukaryotic PHPT (protein histidine phosphatase) is still very limited. To date, only one vertebrate PHPT has been discovered, and two crystal structures of human PHPT1 have been solved. PHPT1/PHP14 can dephosphorylate a variety of proteins (e.g. ATP-citrate lyase and the beta-subunit of G proteins). A putative active site has been identified by its electrostatic character, ion binding, and conserved protein residues.

References

Busam, R.D. et al., 2006, J Biol Chem. 281 (45):33830-4. Zhang, X.Q. et al., 2009, Ups J Med Sci.114 (2):65-72. Gong, W. et al., 2009, Biochem J. 418 (2):337-44. Chapin, L.J. et al., 2009, J Exp Bot. 60 (7):2179-90.

NCBI and Uniprot Product Information

• NCBI GI #: 25008934
• NCBI GeneID: 29085
• UniProt Accession #: Q9NRX4; Q9H0Y3; B1AMX0; B1AMX1
• Molecular Weight: 125
• NCBI Official Full Name: 14 kDa phosphohistidine phosphatase
• NCBI Official Synonym Full Names: phosphohistidine phosphatase 1
• NCBI Official Symbol: PHPT1
• NCBI Official Synonym Symbols: PHP14; CGI-202; HSPC141; HEL-S-132P
• NCBI Protein Information: 14 kDa phosphohistidine phosphatase; protein janus-A homolog; sex-regulated protein janus-a; epididymis secretory sperm binding protein Li 132P
• UniProt Protein Name: 14 kDa phosphohistidine phosphatase
• Protein Family: 14 kDa phosphohistidine phosphatase
• UniProt Gene Name: PHPT1
• UniProt Synonym Gene Names: PHP14
• UniProt Entry Name: PHP14_HUMAN

NCBI Description

This gene encodes an enzyme that catalyzes the reversible dephosphorylation of histidine residues in proteins. It may be involved in the dephosphorylation of G-beta and ATP citrate lyase and in negatively regulating CD4 T lymphocytes by dephosphorylation and inhibition of KCa3.1 channels. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2013]

Uniprot Description

PHPT1: a phosphohistidine phosphatase. Present preferentially in heart and skeletal muscle. Insensitive to inhibition by okadaic acid and EDTA.

Protein type: Carbohydrate Metabolism - fructose and mannose; Phosphatase; Cofactor and Vitamin Metabolism - riboflavin; EC 3.1.3.-; Motility/polarity/chemotaxis; Cofactor and Vitamin Metabolism - thiamine

Chromosomal Location of Human Ortholog: 9q34.3

Cellular Component: cytosol

Molecular Function: phosphohistidine phosphatase activity; calcium channel inhibitor activity; phosphoprotein phosphatase activity

Biological Process: negative regulation of lyase activity; negative regulation of T cell receptor signaling pathway; protein amino acid dephosphorylation