Rabbit anti-Human, Mouse ATP4B Polyclonal Antibody | anti-ATP4B antibody

ATP4B, NT (ATP4B, Potassium-transporting ATPase subunit beta, Gastric H(+)/K(+) ATPase subunit beta, Proton pump beta chain) (PE)

Cat. Num. AAA6276490

• Gene Names: ATP4B; ATP6B
• Reactivity: Human, Mouse
• Applications: Flow Cytometry, Functional Assay, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: ATP4B; Polyclonal Antibody; NT (ATP4B; Potassium-transporting ATPase subunit beta; Gastric H(+)/K(+) ATPase subunit beta; Proton pump beta chain) (PE); Proton pump beta chain; anti-ATP4B antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-ATP4B antibody: Flow Cytometry (FC/FACS), FLISA, Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: ATP4B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 60-90 amino acids from the N-terminal region of human ATP4B.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of ATP4B Antibody (N-term) in mouse heart tissue lysates (35ug/lane).ATP4B (arrow) was detected using the purified Pab.)

Flow Cytometry (FC/FACS)

(ATP4B Antibody (N-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-ATP4B antibody

ATP4B belongs to a family of P-type cation-transporting ATPases. The gastric H+, K+-ATPase is a heterodimer consisting of a high molecular weight catalytic alpha subunit and a smaller but heavily glycosylated beta subunit. This enzyme is a proton pump that catalyzes the hydrolysis of ATP coupled with the exchange of H(+) and K(+) ions across the plasma membrane. It is also responsible for gastric acid secretion. This gene encodes the beta subunit of the gastric H+, K+-ATPase.

Product Categories/Family for anti-ATP4B antibody

Antibodies; Ion Channel

References

Bab-Dinitz, E., et al. Biochemistry 48(36):8684-8691(2009). Knouff, C.W., et al. Pharmacogenet. Genomics 18(12):1051-1057(2008). Oh, J.H., et al. Mamm. Genome 16(12):942-954(2005).

NCBI and Uniprot Product Information

• NCBI GI #: 4557339
• NCBI GeneID: 496
• NCBI Accession #: NP_000696.1
• NCBI GenBank Nucleotide #: NM_000705.3
• UniProt Accession #: P51164; B1B0N8
• Molecular Weight: 33,367 Da
• NCBI Official Full Name: potassium-transporting ATPase subunit beta
• NCBI Official Synonym Full Names: ATPase H+/K+ transporting beta subunit
• NCBI Official Symbol: ATP4B
• NCBI Official Synonym Symbols: ATP6B
• NCBI Protein Information: potassium-transporting ATPase subunit beta
• UniProt Protein Name: Potassium-transporting ATPase subunit beta
• Protein Family: Potassium-transporting ATPase
• UniProt Gene Name: ATP4B

NCBI Description

The protein encoded by this gene belongs to a family of P-type cation-transporting ATPases. The gastric H+, K+-ATPase is a heterodimer consisting of a high molecular weight catalytic alpha subunit and a smaller but heavily glycosylated beta subunit. This enzyme is a proton pump that catalyzes the hydrolysis of ATP coupled with the exchange of H(+) and K(+) ions across the plasma membrane. It is also responsible for gastric acid secretion. This gene encodes the beta subunit of the gastric H+, K+-ATPase. [provided by RefSeq, Jul 2008]

Uniprot Description

ATP4B: belongs to a family of P-type cation-transporting ATPases. The gastric H+, K+-ATPase is a heterodimer consisting of a high molecular weight catalytic alpha subunit and a smaller but heavily glycosylated beta subunit. This enzyme is a proton pump that catalyzes the hydrolysis of ATP coupled with the exchange of H(+) and K(+) ions across the plasma membrane. It is also responsible for gastric acid secretion. This gene encodes the beta subunit of the gastric H+, K+-ATPase. [provided by RefSeq, Jul 2008]

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 13q34

Cellular Component: plasma membrane

Molecular Function: hydrogen:potassium-exchanging ATPase activity; protein binding