Rabbit ATP2C1 Polyclonal Antibody | anti-ATP2C1 antibody

ATP2C1 Polyclonal Antibody

Cat. Num. AAA3011258

• Gene Names: ATP2C1; HHD; BCPM; PMR1; SPCA1; hSPCA1; ATP2C1A
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: ATP2C1; Polyclonal Antibody; ATP2C1 Polyclonal Antibody; ATP dependent Ca(2+) pump PMR1; ATP-dependent Ca(2+) pump PMR1; ATP2C1A; ATPase 2C1; ATPase Ca(2+) sequestering; ATPase Ca++ transporting type 2C member 1; AW061228; BCPM; Calcium transporting ATPase type 2C member 1; Calcium-transporting ATPase type 2C member 1; D930003G21Rik; HHD; hSPCA1; HUSSY 28; KIAA1347; PMR1; rat; homolog of; PMR1L; Secretory pathway Ca(2+) ATPase 1; Secretory pathway Ca(2+)-transporting ATPase; Secretory pathway Ca2+/Mn2+ ATPase 1; SPCA; SPCA1; anti-ATP2C1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: ATP2C1 polyclonal antibody detects endogenous levels of ATP2C1 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 919

• Applicable Applications for anti-ATP2C1 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200; IF: 1:50-1:200
• Immunogen: Recombinant full length Human ATP2C1.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded mouse lung using ATP2C1 antibody at dilution of 1:100 (x40 lens).)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded rat brain using ATP2C1 antibody at dilution of 1:100 (x40 lens).)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human stomach using ATP2C1 antibody at dilution of 1:100 (x40 lens).)

Western Blot (WB)

(Western Blot analysis of extracts of various cell lines, using ATP2C1 Antibody.)

Related Product Information for anti-ATP2C1 antibody

The Saccharomyces cerevisiae protein, PMR1, encodes P-type calcium transport ATPase, which localizes to the Golgi and regulates the intracellular transport of calcium and manganese. The human homologue, ATP2C1 (also designated SPLA in rat), also regulates the transport of calcium in the Golgi complex and is related to other P-type ATPases family members, such as the sarco(endo)plasmic calcium ATPase (SERCA) and the plasma membrane calcium ATPase (PCMA). PMR1 is a transmembrane protein that exists as two splice variants, which vary by 20 amino acids. PMR1 is mutated in Hailey-Hailey disease (HHD), which is an autosomal dominant disorder that is characterized by blisters and erosions of the skin. These findings provide further evidence that PMR1 plays a key role in maintaining the integrity of the epidermis by controlling intracellular calcium signaling.

NCBI and Uniprot Product Information

• NCBI GI #: 68068024
• NCBI GeneID: 27032
• UniProt Accession #: P98194
• Molecular Weight: ~ 125kDa
• NCBI Official Full Name: Calcium-transporting ATPase type 2C member 1
• NCBI Official Synonym Full Names: ATPase secretory pathway Ca2+ transporting 1
• NCBI Official Symbol: ATP2C1
• NCBI Official Synonym Symbols: HHD; BCPM; PMR1; SPCA1; hSPCA1; ATP2C1A
• NCBI Protein Information: calcium-transporting ATPase type 2C member 1
• UniProt Protein Name: Calcium-transporting ATPase type 2C member 1
• Protein Family: Calcium-transporting ATPase
• UniProt Gene Name: ATP2C1
• UniProt Synonym Gene Names: KIAA1347; PMR1L; ATPase 2C1
• UniProt Entry Name: AT2C1_HUMAN

NCBI Description

The protein encoded by this gene belongs to the family of P-type cation transport ATPases. This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium ions. Defects in this gene cause Hailey-Hailey disease, an autosomal dominant disorder. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Aug 2011]

Uniprot Description

ATP2C1: This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of the calcium. Defects in ATP2C1 are the cause of Hailey-Hailey disease (HHD); also known as benign familial pemphigus. HHD is an autosomal dominant disorder characterized by persistent blisters and suprabasal cell separation (acantholysis) of the epidermis, due to impaired keratinocyte adhesion. Patients lacking all isoforms except isoform 2 have HHD. Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIA subfamily. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Transporter; Hydrolase; Membrane protein, multi-pass; Membrane protein, integral; Transporter, ion channel; EC 3.6.3.8

Chromosomal Location of Human Ortholog: 3q22.1

Cellular Component: Golgi membrane; Golgi apparatus; membrane; integral to membrane; trans-Golgi network

Molecular Function: manganese-transporting ATPase activity; signal transducer activity; calcium-transporting ATPase activity; manganese ion binding; metal ion binding; calcium ion binding; ATP binding

Biological Process: cellular calcium ion homeostasis; Golgi calcium ion homeostasis; epidermis development; positive regulation of I-kappaB kinase/NF-kappaB cascade; metabolic process; calcium ion transport; calcium-dependent cell-cell adhesion; actin cytoskeleton reorganization; manganese ion transport; cellular manganese ion homeostasis; signal transduction; Golgi calcium ion transport; transmembrane transport

Disease: Benign Chronic Pemphigus