Rabbit anti-Human, Mouse ATP2C1 Polyclonal Antibody | anti-ATP2C1 antibody

ATP2C1 Antibody

Cat. Num. AAA151043

• Gene Names: ATP2C1; HHD; BCPM; PMR1; SPCA1; hSPCA1; ATP2C1A
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: ATP2C1 Antibody is affinity chromatography purified via peptide column.
• Synonyms: ATP2C1; Polyclonal Antibody; ATP2C1 Antibody; HHD; BCPM; PMR1; SPCA1; hSPCA1; ATP2C1A; KIAA1347; PMR1L; HUSSY-28; Calcium-transporting ATPase type 2C member 1; ATPase 2C1; ATPase; Ca++ transporting; type 2C; member 1; anti-ATP2C1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: At least four isoforms of ATP2C1 are known to exist; this antibody will recognize only the three longest isoforms. ATP2C1 antibody will not cross-react with ATP2C2.
• Purity/Purification: ATP2C1 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 973

• Applicable Applications for anti-ATP2C1 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: ATP2C1 antibody can be used for detection of ATP2C1 by Western blot at 1 mug/mL. Antibody can also be used for immunohistochemistry starting at 5 mug/mL. For immunofluorescence start at 20 mug/mL.
• Conjugate: Unconjugated
• Immunogen: ATP2C1 antibody was raised against a 19 amino acid synthetic peptide near the carboxy terminus of human ATP2C1.
• Buffer: ATP2C1 Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: ATP2C1 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of ATP2C1 in mouse brain tissue lysate with ATP2C1 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.)

Immunohistochemistry (IHC)

(Immunohistochemistry of ATP2C1 in mouse brain tissue with ATP2C1 antibody at 5 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of ATP2C1 in mouse brain tissue with ATP2C1 antibody at 20 μg/mL.)

Related Product Information for anti-ATP2C1 antibody

ATP2C1 Antibody: ATP2C1, also known as secretory pathway Ca2+/Mn2+-ATPase (SPCA) 1, belongs to the family of P-type cation transport ATPases. This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of the calcium from the cytosol to the Golgi lumen. Defects in this gene cause Hailey-Hailey disease, an autosomal dominant disorder characterized by persistent blisters and erosions of the skin. Unlike the related protein ATP2C2, ATP2C1 is ubiquitously expressed and displays a lower maximal turnover rate for overall Ca2+-ATPase reaction and a higher apparent affinity for cytosolic Ca2+ activation of phosphorylation. Recent evidence suggests that ATP2C1 is a key regulator of insulin-like growth factor receptor (IGF1R) processing in tumor progression in basal breast cancers.

NCBI and Uniprot Product Information

• NCBI GI #: 312836765
• NCBI GeneID: 27032
• NCBI Accession #: NP_001001486
• NCBI GenBank Nucleotide #: NM_001199180.1
• UniProt Accession #: P98194; O76005; Q86V72; Q86V73; Q8N6V1; Q8NCJ7; B2RAT7; B4DSW3; B7Z3X9; G3XAH8; G8JLN9
• Molecular Weight: 104,032 Da
• NCBI Official Full Name: calcium-transporting ATPase type 2C member 1 isoform 2a
• NCBI Official Synonym Full Names: ATPase, Ca++ transporting, type 2C, member 1
• NCBI Official Symbol: ATP2C1
• NCBI Official Synonym Symbols: HHD; BCPM; PMR1; SPCA1; hSPCA1; ATP2C1A
• NCBI Protein Information: calcium-transporting ATPase type 2C member 1; HUSSY-28; ATPase 2C1; ATPase, Ca(2+)-sequestering; ATP-dependent Ca(2+) pump PMR1; secretory pathway Ca2+/Mn2+ ATPase 1
• UniProt Protein Name: Calcium-transporting ATPase type 2C member 1
• Protein Family: Calcium-transporting ATPase
• UniProt Gene Name: ATP2C1
• UniProt Synonym Gene Names: KIAA1347; PMR1L; ATPase 2C1
• UniProt Entry Name: AT2C1_HUMAN

NCBI Description

The protein encoded by this gene belongs to the family of P-type cation transport ATPases. This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium ions. Defects in this gene cause Hailey-Hailey disease, an autosomal dominant disorder. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Aug 2011]

Uniprot Description

ATP2C1: This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of the calcium. Defects in ATP2C1 are the cause of Hailey-Hailey disease (HHD); also known as benign familial pemphigus. HHD is an autosomal dominant disorder characterized by persistent blisters and suprabasal cell separation (acantholysis) of the epidermis, due to impaired keratinocyte adhesion. Patients lacking all isoforms except isoform 2 have HHD. Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIA subfamily. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Transporter; Transporter, ion channel; EC 3.6.3.8; Membrane protein, multi-pass; Membrane protein, integral; Hydrolase

Chromosomal Location of Human Ortholog: 3q22.1

Cellular Component: Golgi membrane; Golgi apparatus; membrane; integral to membrane; trans-Golgi network

Molecular Function: manganese-transporting ATPase activity; signal transducer activity; calcium-transporting ATPase activity; manganese ion binding; metal ion binding; calcium ion binding; ATP binding

Biological Process: epidermis development; positive regulation of I-kappaB kinase/NF-kappaB cascade; metabolic process; manganese ion transport; signal transduction; cellular calcium ion homeostasis; Golgi calcium ion homeostasis; calcium ion transport; calcium-dependent cell-cell adhesion; actin cytoskeleton reorganization; cellular manganese ion homeostasis; Golgi calcium ion transport; transmembrane transport

Disease: Benign Chronic Pemphigus