Western Blot (WB)
(Figure 1: Western blot analysis using ATP2C1 mAb against human ATP2C1 (AA: 119-269) recombinant protein. (Expected MW is 41.7 kDa))
Mouse ATP2C1 Monoclonal Antibody | anti-ATP2C1 antibody
ATP2C1 Antibody
Gene Names
ATP2C1; HHD; BCPM; PMR1; SPCA1; hSPCA1; ATP2C1A
Reactivity
Human, Monkey
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
Affinity-Chromatography
Synonyms
ATP2C1; Monoclonal Antibody; ATP2C1 Antibody; 1700121J11Rik; AT2C1_HUMAN; ATP dependent Ca(2+) pump PMR1; ATP-dependent Ca(2+) pump PMR1; ATP2C1A; ATPase 2C1; ATPase Ca(2+) sequestering; ATPase Ca++ transporting type 2C member 1; AW061228; BCPM; Calcium transporting ATPase type 2C member 1; Calcium-transporting ATPase type 2C member 1; D930003G21Rik; HHD; hSPCA1; HUSSY 28; KIAA1347; MGC58010; MGC93231; OTTHUMP00000216066; OTTHUMP00000216068; OTTHUMP00000216069; OTTHUMP00000216071; OTTHUMP00000216072; OTTHUMP00000216073; OTTHUMP00000216074; OTTHUMP00000216075; PMR1; rat; homolog of; PMR1L; Secretory pathway Ca(2+) ATPase 1; Secretory pathway Ca(2+)-transporting ATPase; Secretory pathway Ca2+/Mn2+ ATPase 1; SPCA; SPCA1; anti-ATP2C1 antibody
Host
Mouse
Reactivity
Human, Monkey
Clonality
Monoclonal
Isotype
IgG1
Specificity
ATP2C1 antibody detects endogenous levels of total ATP2C1
Purity/Purification
Affinity-Chromatography
Form/Format
Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
888
Applicable Applications for anti-ATP2C1 antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
ELISA: 1:10000
WB: 1:500-1:2000
IHC: 1:200-1:1000
WB: 1:500-1:2000
IHC: 1:200-1:1000
Immunogen
Purified recombinant fragment of human ATP2C1 expressed in E. Coli
Subcellular Location
Golgi Apparatus Membrane.
Tissue Specificity
Found in most tissues except colon, thymus, spleen and leukocytes. Most abundant in keratinocytes and kidney.
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Figure 1: Western blot analysis using ATP2C1 mAb against human ATP2C1 (AA: 119-269) recombinant protein. (Expected MW is 41.7 kDa))
Western Blot (WB)
(Figure 1: Western blot analysis using ATP2C1 mAb against human ATP2C1 (AA: 119-269) recombinant protein. (Expected MW is 41.7 kDa))
Related Product Information for anti-ATP2C1 antibody
Description: ATP2C1, also known as PMR1, it belongs to the family of P-type cation transport ATPases. This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of the calcium. The human homologue, ATP2C1 (also designated SPLA in rat), also regulates the transport of calcium in the Golgi complex and is related to other P-type ATPases family members, such as the sarco(endo)plasmic calcium ATPase (SERCA) and the plasma membrane calcium ATPase (PCMA). ATP2C1 is a transmembrane protein that exists as two splice variants, which vary by 20 amino acids. Defects in ATP2C1 cause Hailey-Hailey disease, which is an autosomal dominant disorder that is characterized by blisters and erosions of the skin. These findings provide further evidence that PMR1 plays a key role in maintaining the integrity of the epidermis by controlling intracellular calcium signaling.
Function: This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of the calcium.
Similarity: Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIA subfamily.
Function: This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of the calcium.
Similarity: Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIA subfamily.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed: 100 kDa
Predicted: 101 kDa
Predicted: 101 kDa
NCBI Official Full Name
calcium-transporting ATPase type 2C member 1 isoform 1c
NCBI Official Synonym Full Names
ATPase secretory pathway Ca2+ transporting 1
NCBI Official Symbol
ATP2C1
NCBI Official Synonym Symbols
HHD; BCPM; PMR1; SPCA1; hSPCA1; ATP2C1A
NCBI Protein Information
calcium-transporting ATPase type 2C member 1
UniProt Protein Name
Calcium-transporting ATPase type 2C member 1
Protein Family
UniProt Gene Name
ATP2C1
UniProt Synonym Gene Names
KIAA1347; PMR1L; ATPase 2C1
NCBI Description
The protein encoded by this gene belongs to the family of P-type cation transport ATPases. This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium ions. Defects in this gene cause Hailey-Hailey disease, an autosomal dominant disorder. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Aug 2011]
Uniprot Description
This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of the calcium.
Research Articles on ATP2C1
Similar Products
Product Notes
The ATP2C1 atp2c1 (Catalog #AAA9602478) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The ATP2C1 Antibody reacts with Human, Monkey and may cross-react with other species as described in the data sheet. AAA Biotech's ATP2C1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). ELISA: 1:10000 WB: 1:500-1:2000 IHC: 1:200-1:1000. Researchers should empirically determine the suitability of the ATP2C1 atp2c1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ATP2C1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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