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Western Blot (WB) (Western blot analysis of extracts of various cell lines, using ATP11B antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 90s.)

Rabbit anti-Human ATP11B Polyclonal Antibody | anti-ATP11B antibody

ATP11B Polyclonal Antibody

Gene Names
ATP11B; ATPIF; ATPIR
Reactivity
Human
Applications
Western Blot
Purity
Affinity Purification
Synonyms
ATP11B; Polyclonal Antibody; ATP11B Polyclonal Antibody; ATPIF; ATPIR; anti-ATP11B antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purification
Sequence Length
1177
Applicable Applications for anti-ATP11B antibody
Western Blot (WB)
Application Notes
WB: 1:500 - 1:2000
Immunogen
Recombinant protein of human ATP11B
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Preparation and Storage
Store at -20 degree C. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using ATP11B antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 90s.)

Western Blot (WB) (Western blot analysis of extracts of various cell lines, using ATP11B antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 90s.)
Related Product Information for anti-ATP11B antibody
P-type ATPases, such as ATP11B, are phosphorylated in their intermediate state and drive uphill transport of ions across membranes. Several subfamilies of P-type ATPases have been identified. One subfamily transports heavy metal ions, such as Cu(2+) or Cd(2+). Another subfamily transports non-heavy metal ions, such as H(+), Na(+), K(+), or Ca(+). A third subfamily transports amphipaths, such as phosphatidylserine.
Product Categories/Family for anti-ATP11B antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
134kDa
NCBI Official Full Name
Probable phospholipid-transporting ATPase IF
NCBI Official Synonym Full Names
ATPase phospholipid transporting 11B (putative)
NCBI Official Symbol
ATP11B
NCBI Official Synonym Symbols
ATPIF; ATPIR
NCBI Protein Information
probable phospholipid-transporting ATPase IF
UniProt Protein Name
Probable phospholipid-transporting ATPase IF
UniProt Gene Name
ATP11B
UniProt Synonym Gene Names
ATPIF; ATPIR; KIAA0956

NCBI Description

P-type ATPases, such as ATP11B, are phosphorylated in their intermediate state and drive uphill transport of ions across membranes. Several subfamilies of P-type ATPases have been identified. One subfamily transports heavy metal ions, such as Cu(2+) or Cd(2+). Another subfamily transports non-heavy metal ions, such as H(+), Na(+), K(+), or Ca(+). A third subfamily transports amphipaths, such as phosphatidylserine.[supplied by OMIM, Feb 2005]

Uniprot Description

ATP11B: Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IV subfamily. Interacts with TMEM30A

Protein type: EC 3.6.3.1; Hydrolase; Membrane protein, integral; Membrane protein, multi-pass; Transporter; Transporter, ion channel

Chromosomal Location of Human Ortholog: 3q26.33

Cellular Component: endoplasmic reticulum; membrane; plasma membrane; recycling endosome

Molecular Function: phospholipid-translocating ATPase activity; protein binding

Biological Process: aminophospholipid transport; neutrophil degranulation

Research Articles on ATP11B

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Product Notes

The ATP11B atp11b (Catalog #AAA9132114) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ATP11B Polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ATP11B can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500 - 1:2000. Researchers should empirically determine the suitability of the ATP11B atp11b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ATP11B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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