Human Bone Morphogenetic Protein 5 (BMP5) ELISA Kit | BMP5 elisa kit

Human Bone Morphogenetic Protein 5 (BMP5) ELISA Kit

Cat. Num. AAA2704175

• Reactivity: Human
• Synonyms: Bone Morphogenetic Protein 5 (BMP5); Human Bone Morphogenetic Protein 5 (BMP5) ELISA Kit; BMP5 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Bone Morphogenetic Protein 5 (BMP5).; No significant cross-reactivity or interference between Bone Morphogenetic Protein 5 (BMP5) and analogues was observed.
• Sequence Length: 454

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue Homogenates and Other Biological Fluids
• Detection Range: 15.62-1000pg/mL
• Sensitivity: < 5.3pg/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Bone Morphogenetic Protein 5 (BMP5) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Bone Morphogenetic Protein 5 (BMP5) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for BMP5 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Bone Morphogenetic Protein 5 (BMP5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Bone Morphogenetic Protein 5 (BMP5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Bone Morphogenetic Protein 5 (BMP5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Bone Morphogenetic Protein 5 (BMP5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 10835091
• NCBI GeneID: 653
• NCBI Accession #: NP_066551.1
• NCBI GenBank Nucleotide #: NM_021073.3
• UniProt Accession #: P22003; Q9H547; Q9NTM5; B4E0Y4
• Molecular Weight: 47,670 Da
• NCBI Official Full Name: bone morphogenetic protein 5 isoform 1 preproprotein
• NCBI Official Synonym Full Names: bone morphogenetic protein 5
• NCBI Official Symbol: BMP5
• NCBI Protein Information: bone morphogenetic protein 5
• UniProt Protein Name: Bone morphogenetic protein 5
• Protein Family: Bone morphogenetic protein
• UniProt Gene Name: BMP5
• UniProt Synonym Gene Names: BMP-5

NCBI Description

This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer, which plays a role in bone and cartilage development. Polymorphisms in this gene may be associated with osteoarthritis in human patients. This gene is differentially regulated in multiple human cancers. This gene encodes distinct protein isoforms that may be similarly proteolytically processed. [provided by RefSeq, Jul 2016]

Uniprot Description

BMP5: Induces cartilage and bone formation. Belongs to the TGF-beta family.

Protein type: Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 6p12.1

Cellular Component: extracellular space; vesicle

Molecular Function: cytokine activity; growth factor activity; transforming growth factor beta receptor binding

Biological Process: BMP signaling pathway; cardiac muscle tissue development; cartilage development; hindbrain development; male genitalia development; negative regulation of aldosterone biosynthetic process; negative regulation of cell proliferation; negative regulation of insulin-like growth factor receptor signaling pathway; neural fold elevation formation; ossification; pattern specification process; pharyngeal system development; positive regulation of epithelial cell proliferation; positive regulation of transcription from RNA polymerase II promoter; regulation of apoptosis; regulation of MAPK cascade; skeletal system development