Rabbit anti-Human Dual specificity protein phosphatase 7 Polyclonal Antibody | anti-DUSP7 antibody

Rabbit anti-human Dual specificity protein phosphatase 7 polyclonal Antibody(DUSP7)

Cat. Num. AAA1492744

• Gene Names: DUSP7; MKPX; PYST2
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Antigen Affinity Purified
• Synonyms: Dual specificity protein phosphatase 7; Polyclonal Antibody; Rabbit anti-human Dual specificity protein phosphatase 7 polyclonal Antibody(DUSP7); Dual specificity protein phosphatase PYST2; DUSP7; PYST2; anti-DUSP7 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Antigen Affinity Purified
• Sequence Length: 419

• Applicable Applications for anti-DUSP7 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Recommended dilution: WB:1:500-2000; IHC:1:20-1:200
• Storage Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Conjugate: Non-conjugated
• Immunogen: Recombinant human Dual specificity protein phosphatase 7 protein (160-419aa)
• Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-47667 / sc-47669 / sc-47670
• Preparation and Storage: Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(All lanes: Dual specificity protein phosphatase 7 antibody at 10ug/ml+HepG2 whole cell lysateSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 45,41 kDaObserved band size: 45 kDa)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human breast cancer using MBS1492744 at dilution 1:100)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human kidney using MBS1492744 at dilution 1:100)

Related Product Information for anti-DUSP7 antibody

Regulates the activity of the MAP kinase family in response to changes in the cellular environment. PYST2-S may act as a negative regulator of PYST2-L although it is unclear whether this is by competing for transcription, translation or activation factors.

NCBI and Uniprot Product Information

• NCBI GI #: 238231453
• NCBI GeneID: 1849
• NCBI Accession #: NP_001938.2
• NCBI GenBank Nucleotide #: NM_001947.3
• UniProt Accession #: Q16829; Q2M3J7; Q8NFJ0
• Molecular Weight: 40,551 Da
• NCBI Official Full Name: dual specificity protein phosphatase 7
• NCBI Official Synonym Full Names: dual specificity phosphatase 7
• NCBI Official Symbol: DUSP7
• NCBI Official Synonym Symbols: MKPX; PYST2
• NCBI Protein Information: dual specificity protein phosphatase 7
• UniProt Protein Name: Dual specificity protein phosphatase 7
• Protein Family: Dual specificity protein phosphatase
• UniProt Gene Name: DUSP7
• UniProt Synonym Gene Names: PYST2
• UniProt Entry Name: DUS7_HUMAN

NCBI Description

Dual-specificity phosphatases (DUSPs) constitute a large heterogeneous subgroup of the type I cysteine-based protein-tyrosine phosphatase superfamily. DUSPs are characterized by their ability to dephosphorylate both tyrosine and serine/threonine residues. DUSP7 belongs to a class of DUSPs, designated MKPs, that dephosphorylate MAPK (mitogen-activated protein kinase) proteins ERK (see MIM 601795), JNK (see MIM 601158), and p38 (see MIM 600289) with specificity distinct from that of individual MKP proteins. MKPs contain a highly conserved C-terminal catalytic domain and an N-terminal Cdc25 (see MIM 116947)-like (CH2) domain. MAPK activation cascades mediate various physiologic processes, including cellular proliferation, apoptosis, differentiation, and stress responses (summary by Patterson et al., 2009 [PubMed 19228121]).[supplied by OMIM, Dec 2009]

Uniprot Description

DUSP7: a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (ERK, JNK, p38). High levels observed in the active state of AML and ALL diseases and in other types of cancer. 2 isoforms are produced by use of alternative promoters.

Protein type: Motility/polarity/chemotaxis; EC 3.1.3.16; EC 3.1.3.48; Protein phosphatase, dual-specificity

Chromosomal Location of Human Ortholog: 3p21

Cellular Component: cytosol; nucleoplasm

Molecular Function: MAP kinase tyrosine/serine/threonine phosphatase activity; protein binding; protein tyrosine phosphatase activity

Biological Process: activation of MAPK activity; activation of MAPKK activity; axon guidance; epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; inactivation of MAPK activity; innate immune response; insulin receptor signaling pathway; MAPKKK cascade; MyD88-dependent toll-like receptor signaling pathway; MyD88-independent toll-like receptor signaling pathway; negative regulation of MAP kinase activity; nerve growth factor receptor signaling pathway; Ras protein signal transduction; small GTPase mediated signal transduction; stress-activated MAPK cascade; toll-like receptor 10 signaling pathway; toll-like receptor 2 signaling pathway; toll-like receptor 3 signaling pathway; toll-like receptor 4 signaling pathway; toll-like receptor 5 signaling pathway; toll-like receptor 9 signaling pathway; toll-like receptor signaling pathway; vascular endothelial growth factor receptor signaling pathway