Rabbit anti-Human, Mouse Atg7 Polyclonal Antibody | anti-Atg7 antibody

Atg7, NT (Autophagy Protein 7, Ubiquitin-activating enzyme E1-like protein, hAGP7, APG7-like, ATG7, APG7L)

Cat. Num. AAA611317

• Gene Names: Atg7; atg7; CG5489; DmelCG5489
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunocytochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Atg7; Polyclonal Antibody; NT (Autophagy Protein 7; Ubiquitin-activating enzyme E1-like protein; hAGP7; APG7-like; ATG7; APG7L); Anti -Atg7; anti-Atg7 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human APG7. Species Crossreactivity: mouse.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.

• Applicable Applications for anti-Atg7 antibody: ELISA (EL/EIA), Western Blot (WB), Immunocytochemistry (ICC)
• Application Notes: Suitable for use in ELISA, Western Blot and Immunocytochemistry.; Dilution: Western Blot: 1-2ug/ml; Immunocytochemistry: 10ug/ml
• Immunogen: Synthetic peptide, 17 amino acid from near the amino terminus of human APG7 (Genbank accession No. NP_006386).
• Positive Control: L1210 cell lysate
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis of APG7 in L1210 cell lysate with MBS611317 at (A) 1ug/ml, (B) 2ug/ml and (C) 4ug/ml.)

Immunohistochemistry (IHC)

(Immunohistochemistry of APG7 in L1210 cells with MBS611317 at 10ug/ml.)

Related Product Information for anti-Atg7 antibody

Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components (1,2). This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1 (3). Another member of the autophagy family of proteins is APG7 which was identified in yeast as a ubiquitin- E1-like enzyme; this function is conserved in the mammalian homolog (4). In mammalian cells, APG7 is essential for autophagy conjugation systems, autophagosome formation, starvation-induced bulk degradation of proteins and organelles (5). It has been suggested that caspase-8 may alter APG7 levels and thus the APG7 program of autophagic cell death (6).

Product Categories/Family for anti-Atg7 antibody

Antibodies; Abs to Autophagy

NCBI and Uniprot Product Information

• NCBI GI #: 24655127
• NCBI GeneID: 37141
• NCBI Accession #: NP_725809.1
• NCBI GenBank Nucleotide #: NM_166298.1
• NCBI Official Full Name: Autophagy-specific gene 7, isoform B
• NCBI Official Synonym Full Names: Autophagy-specific gene 7
• NCBI Official Symbol: Atg7
• NCBI Official Synonym Symbols: atg7; CG5489; DmelCG5489
• NCBI Protein Information: CG5489 gene product from transcript CG5489-RB; Autophagy-specific gene 7, isoform B; CG5489-PB; Atg7-PB; Autophagy-specific gene 7, isoform A; CG5489-PA; Atg7-PA
• Protein Family: Ubiquitin-like modifier-activating enzyme