Rabbit anti-Human, Mouse APG7 Polyclonal Antibody | anti-APG7 antibody

APG7 Antibody

Cat. Num. AAA9404165

• Gene Names: ATG7; GSA7; APG7L; APG7-LIKE
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunocytochemistry
• Purity: Affinity chromatography purified via peptide column
• Synonyms: APG7; Polyclonal Antibody; APG7 Antibody; Autophagy protein 7; ATG7; anti-APG7 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Purity/Purification: Affinity chromatography purified via peptide column
• Form/Format: Supplied in PBS containing 0.02% sodium azide.
• Sequence Length: 703

• Applicable Applications for anti-APG7 antibody: ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC)
• Immunogen Type: Peptide
• Immunogen Description: Raised against a 17 amino acid peptide from near the amino terminus of human APG7.
• Target Name: APG7
• Preparation and Storage: Can be stored at -20 degree C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of APG7 in L1210 cell lysate with APG7 antibody at (A) 1, (B) 2 and (C) 4 ug/mL.)

Immunocytochemistry (ICC)

(Immunocytochemistry of APG7 in L1210 cells with APG7 antibody at 10 ug/mL.)

Related Product Information for anti-APG7 antibody

Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1. Another member of the autophagy family of proteins is APG7 which was identified in yeast as a ubiquitin-E1-like enzyme; this function is conserved in the mammalian homolog. In mammalian cells, APG7 is essential for autophagy conjugation systems, autophagosome formation, starvation-induced bulk degradation of proteins and organelles. It has been suggested that caspase-8 may alter APG7 levels and thus the APG7 program of autophagic cell death.

Product Categories/Family for anti-APG7 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 5453668
• NCBI GeneID: 10533
• NCBI Accession #: NP_006386
• NCBI GenBank Nucleotide #: NM_006395.2
• Molecular Weight: 68,618 Da
• NCBI Official Full Name: ubiquitin-like modifier-activating enzyme ATG7 isoform a
• NCBI Official Synonym Full Names: autophagy related 7
• NCBI Official Symbol: ATG7
• NCBI Official Synonym Symbols: GSA7; APG7L; APG7-LIKE
• NCBI Protein Information: ubiquitin-like modifier-activating enzyme ATG7
• UniProt Protein Name: Ubiquitin-like modifier-activating enzyme ATG7
• UniProt Gene Name: ATG7
• UniProt Synonym Gene Names: APG7L; APG7-like; hAGP7
• UniProt Entry Name: ATG7_HUMAN

NCBI Description

This gene encodes an E1-like activating enzyme that is essential for autophagy and cytoplasmic to vacuole transport. The encoded protein is also thought to modulate p53-dependent cell cycle pathways during prolonged metabolic stress. It has been associated with multiple functions, including axon membrane trafficking, axonal homeostasis, mitophagy, adipose differentiation, and hematopoietic stem cell maintenance. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]

Uniprot Description

ATG7: Functions as an E1 enzyme essential for multisubstrates such as ATG8-like proteins and ATG12. Forms intermediate conjugates with ATG8-like proteins (GABARAP, GABARAPL1, GABARAPL2 or MAP1LC3A). PE-conjugation to ATG8-like proteins is essential for autophagy. Also acts as an E1 enzyme for ATG12 conjugation to ATG5 and ATG3. Homodimer. Interacts with ATG3 and ATG12. The complex, composed of ATG3 and ATG7, plays a role in the conjugation of ATG12 to ATG5. Widely expressed, especially in kidney, liver, lymph nodes and bone marrow. Belongs to the ATG7 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Autophagy; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 3p25.3

Cellular Component: axoneme; cytoplasm; cytosol

Molecular Function: APG12 activating enzyme activity; APG8 activating enzyme activity; protein binding; protein homodimerization activity; transcription factor binding; ubiquitin activating enzyme activity

Biological Process: adult walking behavior; amino acid metabolic process; C-terminal protein lipidation; cardiac muscle cell development; cellular response to nitrogen starvation; cellular response to starvation; central nervous system neuron axonogenesis; cerebellar Purkinje cell layer development; cerebral cortex development; defense response to virus; liver development; macroautophagy; mitochondrion degradation; negative regulation of apoptosis; neurological system process; positive regulation of apoptosis; positive regulation of autophagy; positive regulation of macroautophagy; positive regulation of protein catabolic process; positive regulation of protein modification process; post-embryonic development; protein amino acid lipidation; protein catabolic process; protein modification process; protein transport; protein ubiquitination; pyramidal neuron development; regulation of protein ubiquitination