Rabbit anti-Human, Mouse APG7 Polyclonal Antibody | anti-ATG7 antibody

APG7 Antibody

Cat. Num. AAA151557

• Gene Names: ATG7; GSA7; APG7L; APG7-LIKE
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunocytochemistry, Immunofluorescence
• Purity: APG7 Antibody is affinity chromatography purified via peptide column.
• Synonyms: APG7; Polyclonal Antibody; APG7 Antibody; GSA7; APG7L; APG7-LIKE; Ubiquitin-like modifier-activating enzyme ATG7; ATG12-activating enzyme E1 ATG7; APG7-like; ATG7 autophagy related 7 homolog (S. cerevisiae); anti-ATG7 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: APG7 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 703

• Applicable Applications for anti-ATG7 antibody: ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: APG7 antibody can be used for the detection of APG7 by Western blot at 0.5 - 2 mug/mL. Antibody can also be used for immunocytochemistry starting at 10 mug/mL. For immunofluorescence start at 20 mug/mL.
• Conjugate: Unconjugated
• Immunogen: APG7 antibody was raised against a 17 amino acid synthetic peptide from near the carboxy terminus of human APG7.
• Buffer: APG7 Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: APG7 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of APG7 in Caco-2 cell lysate with APG7 antibody at (A) 0.5, (B) 1 and (C) 2 μg/mL.)

Immunocytochemistry (IHC)

(Immunocytochemistry of APG7 in MCF7 cells with APG7 antibody at 10 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of APG7 in MCF7 cells with APG7 antibody at 20 μg/mL.)

Related Product Information for anti-ATG7 antibody

APG7 Antibody: Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1. Another member of the autophagy family of proteins is APG7 which was identified in yeast as a ubiquitin-E1-like enzyme; this function is conserved in the mammalian homolog. In mammalian cells, APG7 is essential for autophagy conjugation systems, autophagosome formation, starvation-induced bulk degradation of proteins and organelles. It has been suggested that caspase-8 may alter APG7 levels and thus the APG7 program of autophagic cell death.

NCBI and Uniprot Product Information

• NCBI GI #: 5453668
• NCBI GeneID: 10533
• NCBI Accession #: NP_006386
• NCBI GenBank Nucleotide #: NM_006395.2
• UniProt Accession #: O95352; Q7L8L0; Q9BWP2; Q9UFH4; B4E170; E9PB95
• Molecular Weight: 68,618 Da
• NCBI Official Full Name: ubiquitin-like modifier-activating enzyme ATG7 isoform a
• NCBI Official Synonym Full Names: autophagy related 7
• NCBI Official Symbol: ATG7
• NCBI Official Synonym Symbols: GSA7; APG7L; APG7-LIKE
• NCBI Protein Information: ubiquitin-like modifier-activating enzyme ATG7; hAGP7; autophagy-related protein 7; ATG12-activating enzyme E1 ATG7; ATG7 autophagy related 7 homolog; ubiquitin activating enzyme E1-like protein; ubiquitin-activating enzyme E1-like protein
• UniProt Protein Name: Ubiquitin-like modifier-activating enzyme ATG7
• Protein Family: Ubiquitin-like modifier-activating enzyme
• UniProt Gene Name: ATG7
• UniProt Synonym Gene Names: APG7L; APG7-like; hAGP7
• UniProt Entry Name: ATG7_HUMAN

NCBI Description

This gene was identified based on homology to Pichia pastoris GSA7 and Saccharomyces cerevisiae APG7. In the yeast, the protein appears to be required for fusion of peroxisomal and vacuolar membranes. The protein shows homology to the ATP-binding and catalytic sites of the E1 ubiquitin activating enzymes. [provided by RefSeq, Jan 2009]

Uniprot Description

ATG7: Functions as an E1 enzyme essential for multisubstrates such as ATG8-like proteins and ATG12. Forms intermediate conjugates with ATG8-like proteins (GABARAP, GABARAPL1, GABARAPL2 or MAP1LC3A). PE-conjugation to ATG8-like proteins is essential for autophagy. Also acts as an E1 enzyme for ATG12 conjugation to ATG5 and ATG3. Homodimer. Interacts with ATG3 and ATG12. The complex, composed of ATG3 and ATG7, plays a role in the conjugation of ATG12 to ATG5. Widely expressed, especially in kidney, liver, lymph nodes and bone marrow. Belongs to the ATG7 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin conjugating system; Autophagy

Chromosomal Location of Human Ortholog: 3p25.3

Cellular Component: cytoplasm; axoneme; cytosol

Molecular Function: protein binding; protein homodimerization activity; APG8 activating enzyme activity; APG12 activating enzyme activity; ubiquitin activating enzyme activity; transcription factor binding

Biological Process: positive regulation of apoptosis; regulation of protein ubiquitination; protein ubiquitination; pyramidal neuron development; adult walking behavior; post-embryonic development; protein transport; cardiac muscle cell development; protein amino acid lipidation; protein catabolic process; positive regulation of autophagy; amino acid metabolic process; central nervous system neuron axonogenesis; cerebellar Purkinje cell layer development; protein modification process; C-terminal protein lipidation; liver development; cellular response to starvation; neurological system process; cellular response to nitrogen starvation; protein modification by small protein conjugation; positive regulation of protein modification process; mitochondrion degradation; positive regulation of protein catabolic process; cerebral cortex development; positive regulation of macroautophagy; negative regulation of apoptosis