Rabbit PIST, Control Peptide Polyclonal Antibody | anti-GOPC antibody

PIST, CT, Control Peptide (PDZ Protein Interacting Specifically with TC10, CFTR-associated ligand, CAL, dJ94G16.2, Fused in Glioblastoma, FIG, Golgi-associated PDZ and Coiled-coil Motif Containing, Golgi Associated PDZ and Coiled Coil Motif Containing Pro

Cat. Num. AAA610728

• Gene Names: GOPC; CAL; FIG; PIST; GOPC1; dJ94G16.2; GOPC
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: PIST; Control Peptide; Polyclonal Antibody; CT; Control Peptide (PDZ Protein Interacting Specifically with TC10; CFTR-associated ligand; CAL; dJ94G16.2; Fused in Glioblastoma; FIG; Golgi-associated PDZ and Coiled-coil Motif Containing; Golgi Associated PDZ and Coiled Coil Motif Containing Pro; Anti -PIST; anti-GOPC antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PIST. Species Crossreactivity: mouse and rat.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.

• Applicable Applications for anti-GOPC antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.; Dilution: Western Blot: 1-2ug/ml
• Immunogen: Synthetic peptide PIST 16aa near the carboxy terminus of human PIST (Genbank accession No. AAG00572).
• Positive Control: Rat colon cell lysate
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis of PIST in rat colon cell lysate with PIST antibody at (A) 1ug/ml, (B) 2ug/ml and (C) 4ug/ml.)

Related Product Information for anti-GOPC antibody

Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be

Product Categories/Family for anti-GOPC antibody

Antibodies; Abs to Golgi Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 9837431
• NCBI GeneID: 57120
• UniProt Accession #: Q9HD26; Q59FS4; Q969U8; A6NM30
• Molecular Weight: 50,520 Da
• NCBI Official Full Name: PIST
• NCBI Official Synonym Full Names: golgi-associated PDZ and coiled-coil motif containing
• NCBI Official Symbol: GOPC
• NCBI Official Synonym Symbols: CAL; FIG; PIST; GOPC1; dJ94G16.2; GOPC
• NCBI Protein Information: Golgi-associated PDZ and coiled-coil motif-containing protein; dJ94G16.2 PIST; fused in glioblastoma; CFTR-associated ligand; PDZ protein interacting specifically with TC10; Golgi associated PDZ and coiled-coil motif containing protein; PDZ/coiled-coil domain binding partner for the rho-family GTPase TC10
• UniProt Protein Name: Golgi-associated PDZ and coiled-coil motif-containing protein
• Protein Family: Golgi-associated PDZ and coiled-coil motif-containing protein
• UniProt Gene Name: GOPC
• UniProt Synonym Gene Names: CAL; FIG
• UniProt Entry Name: GOPC_HUMAN

NCBI Description

PIST is a PDZ domain-containing Golgi protein. PDZ domains contain approximately 90 amino acids and bind the extreme C terminus of proteins in a sequence-specific manner.[supplied by OMIM]

Uniprot Description

PIST: a ubiquitously expressed peripheral membrane protein that contains a PDZ domain and two coiled-coil regions. Associates with the Golgi apparatus and plasma membrane, regulating intracellular protein trafficking and degradation. May play a role in autophagy, and regulate the intracellular trafficking of the ADRB1 receptor. Interacts with GOLGA3 and STX6. The PDZ domain is known to mediate interactions with CLCN3-iso2, ACCN3, CFTR, SSTR5, FDZ5, ADRB1, FZD8, GRID2, mGluR5, mGluR1. Mutations of PDZ residues S302D, T304E, K348D, K350E abrogates the ability of PIST to interact with the CFTR C terminus. May regulate CFTR chloride currents and acid-induced ACCN3 currents by modulating cell surface expression of both channels. Overexpression results in CFTR intracellular retention and degradation in the lysosomes. Enriched in synaptosomal and postsynaptic densities (PSD) fractions. Expressed in cell bodies and dendrites of Purkinje cells. Localized at the trans-Golgi network (TGN) of spermatids and the medulla of round spermatides. An oncogenic fusion protein between PIST (aka FIG) and the receptor tyrosine kinase ROS is found in glioblastoma multiform. Unlike other fusion RTK oncogenes, he mechanism of activation of PIST-ROS does not appear to be dimerization. Rather, activation of the fused ROS kinase appears to depend upon translocation to the golgi apparatus: deletion of 2nd coiled-coil region, crucial for Golgi localization, appears to eliminate the transformation capacity of PIST-ROS. Three alternatively spliced human isoforms have been described.

Protein type: Membrane protein, peripheral

Chromosomal Location of Human Ortholog: 6q21

Cellular Component: Golgi membrane; Golgi apparatus; postsynaptic membrane; trans-Golgi network transport vesicle; protein complex; membrane; dendrite; cytoplasm; postsynaptic density; plasma membrane; cell junction

Molecular Function: protein C-terminus binding; protein binding; protein homodimerization activity; frizzled binding

Biological Process: Golgi to plasma membrane transport; ER to Golgi vesicle-mediated transport; protein transport; apical protein localization; regulation of catalytic activity; spermatid nuclear differentiation; protein homooligomerization; cytoplasmic sequestering of CFTR protein

Disease: Spermatogenic Failure 6