Rabbit CPM Polyclonal Antibody | anti-CPM antibody

CPM (L113) Polyclonal Antibody

Cat. Num. AAA3004335

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: CPM; Polyclonal Antibody; CPM (L113) Polyclonal Antibody; Carboxypeptidase M; CBPM; Renal carboxypeptidase; Urinary carboxypeptidase B; anti-CPM antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: CPM (L113) polyclonal antibody detects endogenous levels of CPM protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 443

• Applicable Applications for anti-CPM antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: Synthetic peptide, corresponding to amino acids 80-130 of Human CPM.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of CPM (L113) pAb at 1:500 dilutionLane1:Hela whole cell lysate (40ug) Lane2:The Uterus tissue lysate of Mouse (40ug) Lane3:The Uterus tissue lysate of Rat (40ug))

Related Product Information for anti-CPM antibody

Carboxypeptidase M (CPM) is a 443 amino acid protein belonging to the metallo-carboxypeptidase (metallo-CP) family. Localized to the cell membrane, CPM contains hydrophobic regions in the N and C- termini and has six potential asparagine-linked glycosylation sites. Functionally, CPM specifically removes C-terminal basic amino acids (arginine or lysine) from proteins and polypeptides and is believed to play a role in monocyte to macrophage differentiation. CPM is also thought to play an important role in the control of peptide hormone and growth factor activity at the cell surface, as well as in the membrane-localized degradation of extracellular proteins. Three isoforms of this protein exist as a result of alternative splicing events.

NCBI and Uniprot Product Information

• NCBI GI #: 53832021
• NCBI GeneID: 1368
• NCBI Accession #: NP_001005502.1
• NCBI GenBank Nucleotide #: NP_001005502.1
• UniProt Accession #: P14384
• Molecular Weight: ~ 51kDa
• NCBI Official Full Name: carboxypeptidase M
• NCBI Official Synonym Full Names: carboxypeptidase M
• NCBI Official Symbol: CPM
• NCBI Protein Information: carboxypeptidase M
• UniProt Protein Name: Carboxypeptidase M
• Protein Family: Carboxypeptidase
• UniProt Gene Name: CPM
• UniProt Synonym Gene Names: CPM
• UniProt Entry Name: CBPM_HUMAN

NCBI Description

The protein encoded by this gene is a membrane-bound arginine/lysine carboxypeptidase. Its expression is associated with monocyte to macrophage differentiation. This encoded protein contains hydrophobic regions at the amino and carboxy termini and has 6 potential asparagine-linked glycosylation sites. The active site residues of carboxypeptidases A and B are conserved in this protein. Three alternatively spliced transcript variants encoding the same protein have been described for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

CPM: Specifically removes C-terminal basic residues (Arg or Lys) from peptides and proteins. It is believed to play important roles in the control of peptide hormone and growth factor activity at the cell surface, and in the membrane-localized degradation of extracellular proteins. Belongs to the peptidase M14 family.

Protein type: Protease; Membrane protein, GPI anchor; EC 3.4.17.12

Chromosomal Location of Human Ortholog: 12q14.3

Cellular Component: cell surface; plasma membrane

Molecular Function: zinc ion binding; carboxypeptidase activity; metallocarboxypeptidase activity

Biological Process: anatomical structure morphogenesis; proteolysis