Rabbit anti-Human ATG14 Polyclonal Antibody | anti-ATG14 antibody

ATG14 Antibody

Cat. Num. AAA7050457

• Gene Names: ATG14; ATG14L; BARKOR; KIAA0831
• Reactivity: Human
• Applications: ELISA, Immunohistochemistry, Immunofluorescence
• Purity: >95%, Protein G purified
• Synonyms: ATG14; Polyclonal Antibody; ATG14 Antibody; Beclin 1-associated autophagy-related key regulator1; Autophagy-related protein 14-like protein; anti-ATG14 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >95%, Protein G purified
• Form/Format: Liquid
• Sequence Length: 492

• Applicable Applications for anti-ATG14 antibody: ELISA (EIA), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: IHC: 1: 20-1: 200; IF: 1: 50-1: 200
• Immunogen: Recombinant Human Beclin 1-associated autophagy-related key regulator protein (1-492AA)
• Preservative: 0.03% Proclin 300
• Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
• Conjugation: Non-conjugated
• Preparation and Storage: Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human pancreatic cancer using MBS7050457 at dilution of 1:100)

Immunofluorescence (IF)

(Immunofluorescent analysis of PC3 cells using MBS7050457 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))

Western Blot (WB)

(Western BlotPositive WB detected in: Rat brain tissue,Mouse heart tissue,Mouse brain tissueAll lanes: ATG14 antibody at 2ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 56,43 KDaObserved band size: 56 KDa)

Related Product Information for anti-ATG14 antibody

Required for both basal and inducible autophagy. Determines the localization of the autophagy-specific PI3-kinase complex PI3KC3-C1 (PubMed: 18843052, PubMed: 19050071). Plays a role in autophagosome formation and MAP1LC3/LC3 conjugation to phosphatidylethanolamine (PubMed: 19270696, PubMed: 20713597). Promotes BECN1 translocation from the trans-Golgi network to autophagosomes (PubMed: 20713597). Enhances PIK3C3 activity in a BECN1-dependent manner. Essential for the autophagy-dependent phosphorylation of BECN1 (PubMed: 23878393). Stimulates the phosphorylation of BECN1, but suppresses the phosphorylation PIK3C3 by AMPK (PubMed: 23878393). Binds to STX17-SNAP29 binary t-SNARE complex on autophagosomes and primes it for VAMP8 interaction to promote autophagosome-endolysosome fusion (PubMed: 25686604). Modulates the hepatic lipid metabolism (By similarity).

NCBI and Uniprot Product Information

• NCBI GI #: 50540545
• NCBI GeneID: 22863
• NCBI Accession #: NP_055739.2
• NCBI GenBank Nucleotide #: NM_014924.4
• UniProt Accession #: Q6ZNE5; O94920; Q32MK7; Q32MK8; A6NJE4; A8K9U5; B7ZWP5
• Molecular Weight: 42,434 Da
• NCBI Official Full Name: beclin 1-associated autophagy-related key regulator
• NCBI Official Synonym Full Names: autophagy related 14
• NCBI Official Symbol: ATG14
• NCBI Official Synonym Symbols: ATG14L; BARKOR; KIAA0831
• NCBI Protein Information: beclin 1-associated autophagy-related key regulator
• UniProt Protein Name: Beclin 1-associated autophagy-related key regulator
• Protein Family: Autophagy-related protein
• UniProt Gene Name: ATG14
• UniProt Synonym Gene Names: Atg14L
• UniProt Entry Name: BAKOR_HUMAN

Uniprot Description

Barkor: Required for both basal and inducible autophagy. Plays a role in autophagosome formation and MAP1LC3/LC3 conjugation to phosphatidylethanolamine. Promotes BECN1 translocation from the trans-Golgi network to autophagosomes. Enhances PIK3C3 activity in a BECN1-dependent manner. Forms a complex with BECN1, PIK3C3 and PIK3R4, but not with UVRAG, nor with KIAA0226/Rubicon. UVRAG and ATG14/Barkor form mutually exclusive complexes with BECN1 through direct competition. The complex containing ATG14 up-regulates autophagy, while the one containing Rubicon down-regulates autophagy. Interacts with PIK3CB. Belongs to the Barkor family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Autophagy

Chromosomal Location of Human Ortholog: 14q22.3

Cellular Component: autophagic vacuole; axoneme; cytoplasm; cytosol; endoplasmic reticulum membrane; nucleus; pre-autophagosomal structure membrane

Molecular Function: protein binding

Biological Process: autophagic vacuole docking; autophagic vacuole formation; cellular response to glucose starvation; cellular response to starvation; endosome to lysosome transport; macroautophagy; negative regulation of protein amino acid phosphorylation; positive regulation of phosphoinositide 3-kinase activity; positive regulation of protein amino acid phosphorylation; regulation of protein amino acid phosphorylation