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Testing Data #1 (Western blot analysis of human liver lysate probed with Rabbit anti Human ATG101 (MBS224305) at 1.0 (A) and 2.0 (B) μgml-1)

Rabbit anti-Mouse, Rat ATG101 Polyclonal Antibody | anti-ATG101 antibody

RABBIT ANTI ATG101

Gene Names
Atg101; AA407441; AU022967; 9430023L20Rik
Reactivity
Mouse, Rat
Applications
Immunohistochemistry, ELISA, Western Blot
Synonyms
ATG101; Polyclonal Antibody; RABBIT ANTI ATG101; anti-ATG101 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
This item recognises human Autophagy-related protein 101 (ATG101), a 218 amino acid protein belonging to the ATG101 family.
Form/Format
Purified
Purified IgG - liquid
Concentration
IgG concentration 1.0mg/ml (varies by lot)
Sequence Length
218
Applicable Applications for anti-ATG101 antibody
Immunohistology Frozen, ELISA (EIA), Western Blot (WB)
Application Notes
ELISA: This product is suitable for use in indirect ELISA applications.
Western Blot: This item detects a band of approximately 29 kDa in Human liver tissue lysates.
Immunohistology - Frozen: Minimum Dilution: 5ug/ml
Western Blotting: Minimum Dilution: 1-2ug/ml
Perservative Stabilisers
0.02% Sodium Azide (NaN3)
Preparation
Immunogen
A synthetic peptide corresponding to 16 amino acid sequence from near the centre of human ATG101
Histology Positive Control Tissue
Target Species
Human
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted.
Shelf Life: 18 months from date of despatch.

Testing Data #1

(Western blot analysis of human liver lysate probed with Rabbit anti Human ATG101 (MBS224305) at 1.0 (A) and 2.0 (B) μgml-1)

Testing Data #1 (Western blot analysis of human liver lysate probed with Rabbit anti Human ATG101 (MBS224305) at 1.0 (A) and 2.0 (B) μgml-1)

Testing Data #2

(Formalin fixed, paraffin embedded mouse liver stained with Rabit anti Human ATG101 (MBS224305) following heat mediated antigen retrieval using sodium citrate buffer (pH6.0))

Testing Data #2 (Formalin fixed, paraffin embedded mouse liver stained with Rabit anti Human ATG101 (MBS224305) following heat mediated antigen retrieval using sodium citrate buffer (pH6.0))
Related Product Information for anti-ATG101 antibody
MBS224305 recognises human Autophagy-related protein 101 (ATG101), a 218 amino acid protein belonging to the ATG101 family.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
25,002 Da
NCBI Official Full Name
autophagy-related protein 101
NCBI Official Synonym Full Names
autophagy related 101
NCBI Official Symbol
Atg101
NCBI Official Synonym Symbols
AA407441; AU022967; 9430023L20Rik
NCBI Protein Information
autophagy-related protein 101
UniProt Protein Name
Autophagy-related protein 101
Protein Family
UniProt Gene Name
Atg101
UniProt Entry Name
ATGA1_MOUSE

Uniprot Description

ATG101: an protein required for autophagosome formation. Interacts with ATG13. Associates with a complex composed of ATG13, ULK1 and RB1CC1. Under starvation conditions it localizes to phagophores, which are preautophagosomal structures. Stabilizes the expression of Atg13 in the cell. Belongs to the ATG101 family.

Protein type: Autophagy

Cellular Component: cytoplasm

Molecular Function: identical protein binding; protein complex binding

Biological Process: autophagy; autophagic vacuole formation

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Product Notes

The ATG101 atg101 (Catalog #AAA224305) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RABBIT ANTI ATG101 reacts with Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's ATG101 can be used in a range of immunoassay formats including, but not limited to, Immunohistology Frozen, ELISA (EIA), Western Blot (WB). ELISA: This product is suitable for use in indirect ELISA applications. Western Blot: This item detects a band of approximately 29 kDa in Human liver tissue lysates. Immunohistology - Frozen: Minimum Dilution: 5ug/ml Western Blotting: Minimum Dilution: 1-2ug/ml. Researchers should empirically determine the suitability of the ATG101 atg101 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ATG101, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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