Rabbit ATG4B Polyclonal Antibody | anti-ATG4B antibody

RABBIT ANTI ATG4B

Cat. Num. AAA223512

• Gene Names: ATG4B; APG4B; AUTL1
• Reactivity: Chicken (Expected from Sequence), Fish (Expected from Sequence), Mouse (Expected from Sequence), Rat (Expected from Sequence), Xenopus (Expected from Sequence), Zebrafish (Expected from Sequence)
• Applications: Immunohistochemistry, Western Blot
• Synonyms: ATG4B; Polyclonal Antibody; RABBIT ANTI ATG4B; anti-ATG4B antibody

• Host: Rabbit
• Reactivity: Chicken (Expected from Sequence), Fish (Expected from Sequence), Mouse (Expected from Sequence), Rat (Expected from Sequence), Xenopus (Expected from Sequence), Zebrafish (Expected from Sequence)
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This item recognises Human Autophagy-related protein 4 homologue B (ATG4B), a 44.3 kDa cytoplasmic protein belonging to the peptidase C54 family. ; ; ATG4B is a protein involved with autophagy, a process for the intracellular destruction of endogenous proteins and damaged cell organelles. ; ; ATG4 is strongly expressed in skeletal muscle and to a lesser extent in heart, brain and pancreatic tissues.
• Form/Format: Purified; Purified IgG - liquid
• Concentration: Total protein concentration 0.5 mg/ml (varies by lot)
• Sequence Length: 393

• Applicable Applications for anti-ATG4B antibody: Immunohistology Paraffin*, Western Blot (WB)
• Application Notes: Immunohistology - Paraffin: Minimum Dilution: 10 ug/ml; Application Note: This product requires antigen retrieval using heat treatment. Citrate buffer, pH 6.0 is recommended for this purpose.; Western Blotting: Minimum Dilution: 1 ug/ml; Maximum Dilution: 3 ug/ml
• Perservative Stabilisers: 0.05% Sodium Azide (NaN3); 0.2% Gelatin
• Immunogen: Synthetic peptide corresponding to a portion of the amino acid sequence between 300-450 of human ATG4 - KLH conjugated.; Histology Positive Control Tissue
• Antiserum Preparation: Antisera to ATG4 were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared from whole serum by affinity chromatography.
• Target Species: Human
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Testing Data #1

(Staining of formalin fixed, paraffin embedded human heart with Rabbit anti Human ATG4B (MBS223512))

Related Product Information for anti-ATG4B antibody

MBS223512 recognises Human Autophagy-related protein 4 homologue B (ATG4B), a 44.3 kDa cytoplasmic protein belonging to the peptidase C54 family. ATG4B is a protein involved with autophagy, a process for the intracellular destruction of endogenous proteins and damaged cell organelles. ATG4 is strongly expressed in skeletal muscle and to a lesser extent in heart, brain and pancreatic tissues.

NCBI and Uniprot Product Information

• NCBI GI #: 47132611
• NCBI GeneID: 23192
• NCBI Accession #: NP_037457.3
• NCBI GenBank Nucleotide #: NM_013325.4
• UniProt Accession #: Q9Y4P1; Q53NU4; Q6ZUV8; Q8WYM9; Q96K07; Q96K96; Q96SZ1; Q9Y2F2; B7WNK2
• Molecular Weight: 42,622 Da
• NCBI Official Full Name: cysteine protease ATG4B isoform a
• NCBI Official Synonym Full Names: autophagy related 4B, cysteine peptidase
• NCBI Official Symbol: ATG4B
• NCBI Official Synonym Symbols: APG4B; AUTL1
• NCBI Protein Information: cysteine protease ATG4B; APG4 autophagy 4 homolog B; ATG4 autophagy related 4 homolog B; AUT-like 1 cysteine endopeptidase; autophagin-1; autophagy-related cysteine endopeptidase 1; autophagy-related protein 4 homolog B; hAPG4B
• UniProt Protein Name: Cysteine protease ATG4B
• Protein Family: Cysteine protease
• UniProt Gene Name: ATG4B
• UniProt Synonym Gene Names: APG4B; AUTL1; KIAA0943; hAPG4B
• UniProt Entry Name: ATG4B_HUMAN

NCBI Description

Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

ATG4B: Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes. Mainly expressed in the skeletal muscle, followed by brain, heart, liver and pancreas. Inhibited by N-ethylmaleimide. Belongs to the peptidase C54 family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.22.-; Autophagy; Protease; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 2q37.3

Cellular Component: cytoplasm; cytosol

Molecular Function: protein binding; cysteine-type endopeptidase activity; endopeptidase activity; cysteine-type peptidase activity

Biological Process: protein delipidation; mitochondrion degradation; positive regulation of protein catabolic process; autophagy; protein processing; C-terminal protein lipidation; protein targeting to membrane; proteolysis; positive regulation of autophagy; cellular response to nitrogen starvation; autophagic vacuole formation