Western Blot (WB)
(Western Blot analysis of ATF5 on different lysates using anti-ATF5 antibody at 1/1,000 dilution. Positive control: Lane 1: Jurkat Lane 2: PC-12)
Rabbit ATF5 Polyclonal Antibody | anti-ATF5 antibody
ATF5 Polyclonal Antibody
Gene Names
ATF5; ATFX; HMFN0395
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation
Purity
ProA affinity purified
Synonyms
ATF5; Polyclonal Antibody; ATF5 Polyclonal Antibody; cAMP-dependent transcription factor ATF-5; Activating transcription factor 5 alpha/beta; Activating transcription factor 5; Activating transcription factor X; AFTA; ATF 5; ATF 7; ATF7; ATFX; BZIP protein ATF7; cAMP dependent transcription factor ATF 5; cAMP dependent transcription factor ATF5; Cyclic AMP dependent transcription factor ATF 5; Cyclic AMP dependent transcription factor ATF5; FLJ34666; HMFN0395; MGC102397; NAP1; NRIF3 associated protein; ODA 10; Transcription factor ATFx; Transcription factor like protein ODA 10; Transcription factor like protein ODA10; anti-ATF5 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
ATF5 polyclonal antibody detects endogenous levels of ATF5 protein.
Purity/Purification
ProA affinity purified
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
282
Applicable Applications for anti-ATF5 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP)
Application Notes
WB: 1:1,000-1:2,000
ICC: 1:50-1:200
IHC: 1:50-1:200
ICC: 1:50-1:200
IHC: 1:50-1:200
Immunogen
recombinant protein
Preparation and Storage
Store at 4 degree C after thawing.
Aliquot store at -20 degree C or -80 degree C.
Avoid repeated freeze/thaw cycles.
Aliquot store at -20 degree C or -80 degree C.
Avoid repeated freeze/thaw cycles.
Western Blot (WB)
(Western Blot analysis of ATF5 on different lysates using anti-ATF5 antibody at 1/1,000 dilution. Positive control: Lane 1: Jurkat Lane 2: PC-12)
Western Blot (WB)
(Western Blot analysis of ATF5 on different lysates using anti-ATF5 antibody at 1/1,000 dilution. Positive control: Lane 1: Jurkat Lane 2: PC-12)
Immunocytochemistry (ICC)
(ICC staining ATF5 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Immunocytochemistry (ICC)
(ICC staining ATF5 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Related Product Information for anti-ATF5 antibody
Eukaryotic gene transcription is regulated by sequence-specific transcription factors that bind modular cis acting promoter and enhancer elements. The ATF/CREB transcription factor family binds the palindromic cAMP response element (CRE) octanucleotide TGACGTCA. The best characterized members of this gene family include CREB-1, CREB-2 (also designated ATF-4), CRE-BPa, LZIP (also designated CREB3 and Luman), CREM-1, CREM-2, ATF-1, ATF-2, ATF-3, ATF-5, ATF-6 and ATF-7. This family of proteins contain highly divergent N-terminal domains, but share a C-terminal leucine zipper for dimerization and DNA binding. ATF-5 (ATFx), which can localize to the cytoplasm or the nucleus, binds DNA as a dimer. It interacts with CCND3 and PTP4A1.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
31kDa
NCBI Official Full Name
cyclic AMP-dependent transcription factor ATF-5
NCBI Official Synonym Full Names
activating transcription factor 5
NCBI Official Symbol
ATF5
NCBI Official Synonym Symbols
ATFX; HMFN0395
NCBI Protein Information
cyclic AMP-dependent transcription factor ATF-5
UniProt Protein Name
Cyclic AMP-dependent transcription factor ATF-5
Protein Family
UniProt Gene Name
ATF5
UniProt Synonym Gene Names
ATFX; cAMP-dependent transcription factor ATF-5
UniProt Entry Name
ATF5_HUMAN
Uniprot Description
ATF-5: Transcriptional activator which binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters and blocks the differentiation of neuroprogenitor cells into neurons. Its transcriptional activity is enhanced by CCND3 and slightly inhibited by CDK4. Belongs to the bZIP family.
Protein type: DNA-binding
Chromosomal Location of Human Ortholog: 19q13.3
Cellular Component: nucleoplasm; transcription factor complex; cytoplasm
Molecular Function: protein binding; heat shock protein binding; transcription corepressor activity
Biological Process: circadian rhythm; regulation of transcription from RNA polymerase II promoter; transcription, DNA-dependent; olfactory bulb interneuron development; multicellular organism growth; negative regulation of astrocyte differentiation; positive regulation of transcription from RNA polymerase II promoter; negative regulation of apoptosis; post-embryonic development
Research Articles on ATF5
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Product Notes
The ATF5 atf5 (Catalog #AAA3012271) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ATF5 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's ATF5 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP). WB: 1:1,000-1:2,000 ICC: 1:50-1:200 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the ATF5 atf5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ATF5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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